A Paenibacillus sp. dextranase mutant pool with improved thermostability and activity

Applied Microbiology and Biotechnology

Volumn 75, Issue 5, 2007, Pages 1071-1078

  Hild, Erika ^a   Brumbley, Stevens M ^b,c   O'Shea, Michael G ^b   Nevalainen, Helena ^a   Bergquist, Peter L ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b BSES LIMITED   (Australia)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Activity; Dextranase; Directed evolution; Paenibacillus sp.; Random mutagenesis; Thermostability

Indexed keywords

GENES; MUTAGENESIS; NUCLEOTIDES; THERMODYNAMIC STABILITY;

DEXTRANASE; DIRECTED EVOLUTION; PAENIBACILLUS SP.; RANDOM MUTAGENESIS;

ENZYMES;

DEXTRANASE;

ASSAY; BACTERIUM; ENZYME ACTIVITY; GENE; MUTATION; TEMPERATURE EFFECT;

AMINO ACID SUBSTITUTION; ARTICLE; BIODIVERSITY; CHIMERA; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE LIBRARY; GENE MUTATION; GENETIC RECOMBINATION; GENETIC SCREENING; GENETIC VARIABILITY; HALF LIFE TIME; MUTAGENESIS; MUTANT; NONHUMAN; PAENIBACILLUS; THERMAL EXPOSURE; THERMOSTABILITY; WILD TYPE;

AMINO ACID SUBSTITUTION; BACILLUS; DEXTRANASE; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; HEAT;

PAENIBACILLUS SP.;

EID: 34250668434     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-0936-6     Document Type: Article

References (33)

1. Aharoni A, Griffiths AD, Tawfik DS (2005) High-throughput screens and selections of enzyme-encoding genes. Curr Opin Chem Biol 9:210-216
2. Akashi H (2001) Gene expression and molecular evolution. Curr Opin Genet Dev 11:660-666
3. Bloom JD, Meyer MM, Meinhold P, Otey CR, MacMillan D, Arnold FH (2005) Evolving strategies for enzyme engineering. Curr Opin Struct Biol 15:447-452
4. Cadwell RC, Joyce GF (1992) Randomization of genes by PCR mutagenesis. PCR Methods Appl 2:28-33
5. Chen FM, Zhao YM, Wu H, Deng ZH, Wang QT, Zhou W, Liu Q, Dong GY, Li K, Wu ZF, Jin Y (2006) Enhancement of peridontal tissue regeneration by locally controlled delivery of insulin-like growth factor-I from dextran-co-gelatin microspheres. J Control Release 114:209-222
6. Eggleston G, Monge A (2005) Optimization of sugarcane factory application of commercial dextranases. Process Biochem 40:1881-1894
7. Eijsink VGH, Gåseidnes S, Borchert TV, Van den Burg B (2005) Directed evolution of enzyme stability. Biomol Eng 22:21-30
8. Finnegan PM, Brumbley SM, O'Shea MG, Nevalainen KMH, Bergquist PL (2004a) Paenibacillus isolates possess diverse dextran-degrading enzymes. J Appl Microbiol 97:477-485
9. Finnegan PM, Brumbley SM, O'Shea MG, Nevalainen H, Bergquist PL (2004b) Isolation and characterization of genes encoding thermoactive and thermostable dextranases from two thermotolerant soil bacteria. Curr Microbiol 49:327-333
10. Finnegan PM, Brumbley SM, O'Shea MG, Nevalainen H, Bergquist PL (2005) Diverse dextranase genes from Paenibacillus species. Arch Microbiol 183:140-147
11. Flores H, Ellington AD (2002) Increasing the thermal stability of an oligomeric protein, beta-glucuronidase. J Mol Biol 315:325-337
12. Giver L, Gershenson A, Freskgard PO, Arnold FH (1998) Directed evolution of a thermostable esterase. Proc Natl Acad Sci U S A 95:12809-12813
13. Hayacibara MF, Koo H, Vacca Smith AM, Kopec LK, Scott-Anne K, Cury JA, Bowen WH (2004) The influence of mutanase and dextranase on the production and structure of glucans synthesized by streptococcal glucosyltransferases. Carbohydr Res 339:2127-2137
14. EG Hibbert PA Dalby 2005 Directed evolution strategies for improved enzymatic performance Microb Cell Fact 4 29 Hibbert EG, Dalby PA (2005) Directed evolution strategies for improved enzymatic performance. Microb Cell Fact 4:29. DOI 10.1186/1475-2859-4-29
15. Hibbert EG, Baganz F, Hailes HC, Ward JM, Lye GJ, Woodley JM, Dalby PA (2005) Directed evolution of biocatalytic processes. Biomol Eng 22:11-19
16. 8-barrel enzymes. Biomol Eng 22:31-38
17. Holmberg RC, Henry AA, Romesberg FE (2005) Directed evolution of novel polymerases. Biomol Eng 22:39-49
18. Ingelman B (1948) Enzymatic beakdown of dextran. Acta Chem Scand 2:803-812
19. Johannes TW, Zhao H (2006) Directed evolution of enzymes and biosynthetic pathways. Curr Opin Microbiol 9:261-267
20. Khalikova E, Susi P, Korpela T (2005) Microbial dextran-hydrolyzing enzymes: fundamentals and applications. Microbiol Mol Biol Rev 69:306-325
21. Kim YW, Choi JH, Kim JW, Park C, Kim JW, Cha H, Lee SB, Oh BH, Moon TW, Park KH (2003) Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance. Appl Environ Microbiol 69:4866-4874
22. Kubic C, Sikora B, Bielecki S (2004) Immobilization of dextransucrase and its use with soluble dextranase for glucooligosaccharides synthesis. Enzyme Microb Technol 34:555-560
23. Kumar S, Halpert JR (2005) Use of directed evolution of mammalian cytochromes P450 for investigating the molecular basis of enzyme function and generating novel biocatalysts. Biochem Biophys Res Commun 338:456-464
24. Larsson AM, Andersson R, Ståhlberg J, Kenne L, Jones TA (2003) Dextranase from Penicillium minioluteum: reaction course, crystal structure, and product complex. Structure 11:1111-1121
25. Lever M (1972) A new reaction for colorimetric determination of carbohydrates. Anal Biochem 47:273-279
26. Marotta M, Martino A, De Rosa A, Farina E, Carteni M, De Rosa M (2002) Degradation of dental plaque glucans and prevention of glucan formation using commercial enzymes. Process Biochem 38:101-108
27. Morisaki H, Igarashi T, Yamamoto A, Goto N (2002) Analysis of a dextran-binding domain of the dextranase of Streptococcus mutans. Lett Appl Microbiol 35:223-227
28. Morley KL, Kazlauskas RJ (2005) Improving enzyme properties: when are closer mutations better? Trends Biotechnol 23:231-237
29. Otten LG, Quax WJ (2005) Directed evolution: selecting today's biocatalysts. Biomol Eng 22:1-9
30. Schauder B, Blöcker H, Frank R, McCarthy JEG (1987) Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. Gene 52:279-283
31. Schmidt-Dannert C, Arnold FH (1999) Directed evolution of enzymes. Trends Biotechnol 17:135-136
32. Zaccolo M, Williams DM, Brown DM, Gherardi E (1996) An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues. J Mol Biol 255:589-603
33. Zhou YH, Zhang X, Ebright RH (1991) Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Nucleic Acids Res 19:6052