Inhibition of PI3-kinase signaling by glucocorticoids results in increased branched-chain amino acid degradation in renal epithelial cells

American Journal of Physiology - Cell Physiology

Volumn 292, Issue 5, 2007, Pages

  Wang, Xiaonan ^a   Hu, Junping ^a   Price, S Russ ^a  

^a EMORY UNIVERSITY   (United States)

Author keywords

Kidney; Phosphatidylinositol 3 kinase

Indexed keywords

2 OXOISOVALERATE DEHYDROGENASE (LIPOAMIDE); BRANCHED CHAIN AMINO ACID; DEXAMETHASONE; GLUCOCORTICOID; INSULIN RECEPTOR SUBSTRATE 1; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN P85; 2 MORPHOLINO 8 PHENYLCHROMONE; 2-(4-MORPHOLINYL)-8-PHENYL-4H-1-BENZOPYRAN-4-ONE; CHROMONE DERIVATIVE; ENZYME INHIBITOR; GLUCOCORTICOID RECEPTOR; INSULIN RECEPTOR SUBSTRATE 1 PROTEIN; INSULIN RECEPTOR SUBSTRATE-1 PROTEIN; MORPHOLINE DERIVATIVE; PHOSPHOPROTEIN; PROTEIN KINASE INHIBITOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME REGULATION; EPITHELIUM CELL; KIDNEY EPITHELIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; TRANSCRIPTION INITIATION; ANIMAL; CELL STRAIN; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; DRUG POTENTIATION; ENZYMOLOGY; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; KIDNEY; METABOLISM; PHOSPHORYLATION; PROMOTER REGION; SWINE;

1-PHOSPHATIDYLINOSITOL 3-KINASE; 3-METHYL-2-OXOBUTANOATE DEHYDROGENASE (LIPOAMIDE); AMINO ACIDS, BRANCHED-CHAIN; ANIMALS; CHROMONES; DEXAMETHASONE; ENZYME INHIBITORS; EPITHELIAL CELLS; GLUCOCORTICOIDS; KIDNEY; LLC-PK1 CELLS; MORPHOLINES; PHOSPHOPROTEINS; PHOSPHORYLATION; PROMOTER REGIONS (GENETICS); PROTEIN KINASE INHIBITORS; PROTO-ONCOGENE PROTEINS C-AKT; RECEPTORS, GLUCOCORTICOID; SIGNAL TRANSDUCTION; SWINE; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 34250637539     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00617.2006     Document Type: Article

References (36)

1. Aftring RP, Miller WJ, Buse MG. Effects of diabetes and starvation on skeletal muscle branched-chain α-keto acid dehydrogenase activity. Am J Physiol Endocrinol Metab 254: E292-E300, 1988.
2. Bailey JL, Zheng B, Hu Z, Price SR, Mitch WE. Chronic kidney disease causes defects in signaling through the insulin receptor substrate/phosphatidylinositol 3-kinase/Akt pathway: implications for muscle atrophy. J Am Soc Nephrol 17: 1388-1394, 2006.
3. Barbour LA, Mizanoor RS, Gurevich I, Leitner JW, Fischer SJ, Roper MD, Knotts TA, Vo Y, McCurdy CE, Yakar S, LeRoith D, Kahn CR, Cantley LC, Friedman JE, Draznin B. Increased P85alpha is a potent negative regulator of skeletal muscle insulin signaling and induces in vivo insulin resistance associated with growth hormone excess. J Biol Chem 280: 37489-37494, 2005.
4. Bergstrom J, Alvestrand A, Furst P. Plasma and muscle free amino acids in maintenance hemodialysis patients without protein malnutrition. Kidney Int 38: 108-114, 1990.
5. Bergstrom J, Furst P, Noree LO, Vinnars E. Intracellular free amino acids in muscle tissue of patients with chronic uraemia: effect of peritoneal dialysis and infusion of essential amino acids. Clin Sci Mol Med 54: 51-60, 1978.
6. Birnbaum MJ. Turning down insulin signaling. J Clin Invest 108: 655-659, 2001.
7. Costeas PA, Chinsky JM. Isolation of the branched-chain α-ketoacid dehydrogenase E2 subunit promoter region. Biochim Biophys Acta 1399: 111-116, 1998.
8. Del Aguila LF, Claffey KP, Kirwan JP. TNF-α impairs insulin signaling and insulin stimulation of glucose uptake in C2C12 muscle cells. Am J Physiol Endocrinol Metab 276: E849-E855, 1999.
9. Franch HA, Raissi S, Wang X, Zheng B, Bailey JL, Price SR. Acidosis impairs insulin receptor substrate-1-associated phosphoinositide 3-kinase signaling in muscle cells: consequences on proteolysis. Am J Physiol Renal Physiol 287: F700-F706, 2004.
10. Franch HA, Wang X, Sooparb S, Brown NS, Du J. Phosphatidylinositol 3-kinase activity is required for epidermal growth factor to suppress proteolysis. J Am Soc Nephrol 13: 903-909, 2002.
11. Giorgino F, Almahfouz A, Goodyear LJ, Smith RJ. Glucocorticoid regulation of insulin receptor and substrate IRS-1 tyrosine phosphorylation in rat skeletal muscle in vivo. J Clin Invest 91: 2020-2030, 1993.
12. Giorgino F, Pedrini MT, Matera L, Smith RJ. Specific increase in p85α expression in response to dexamethasone is associated with inhibition of insulin-like growth factor-I stimulated phosphatidylinositol 3-kinase activity in cultured muscle cells. J Biol Chem 272: 7455-7463, 1997.
13. Gual P, Le Marchand-Brustel Y, Tanti JF. Positive and negative regulation of insulin signaling through IRS-1 phosphorylation. Biochimie 87: 99-109, 2005.
14. Hara Y, May RC, Kelly RA, Mitch WE. Acidosis, not azotemia, stimulates branched-chain amino acid catabolism in uremic rats. Kidney Int 32: 808-814, 1987.
15. Helms MN, Liu L, Liang YY, Al-Khalili O, Vandewalle A, Saxena S, Eaton DC, Ma HP. Phosphatidylinositol 3,4,5-trisphosphate mediates aldosterone stimulation of epithelial sodium channel (ENaC) and interacts with gamma-ENaC. J Biol Chem 280: 40885-40891, 2005.
16. Huang YS, Chuang DT. Mechanism for basal expression of rat mitochondrial branched-chain-2-oxo-acid dehydrogenase kinase. Biochem J 334: 713-722, 1998.
17. Ibrahim NM, Marinovic AC, Price SR, Young LG, Frohlich O. Pitfall of an internal control plasmid: response of Renilla luciferase (pRL-TK) plasmid to dihydrotestosterone and dexamethasone. Biotechniques 29: 782-784, 2000.
18. Kwon DS, Kwon CH, Kim JH, Woo JS, Jung JS, Kim YK. Signal transduction of MEK/ERK and PI3K/Akt activation by hypoxia/reoxygenation in renal epithelial cells. Eur J Cell Biol 85: 1189-1199, 2006.
19. Lee SW, Dai G, Hu Z, Wang X, Du J, Mitch WE. Regulation of muscle protein degradation: coordinated control of apoptotic and ubiquitin-proteasome systems by phosphatidylinositol 3 kinase. J Am Soc Nephrol 15: 1537-1545, 2004.
20. Lombardo YB, Serdikoff C, Thamotharan M, Paul HS, Adibi SA. Inverse alterations of BCKA dehydrogenase activity in cardiac and skeletal muscles of diabetic rats. Am J Physiol Endocrinol Metab 277: E685-E692, 1999.
21. May RC, Bailey JL, Mitch WE, England BK. Glucocorticoids and acidosis stimulate protein and amino acid catabolism in vivo. Kidney Int 49: 679-683, 1996.
22. May RC, Hara Y, Kelly RA, Brock KP, Buse MG, Mitch WE. Branched-chain amino acid metabolism in rat muscle: abnormal regulation in acidosis. Am J Physiol Endocrinol Metab 252: E712-E718, 1987.
23. May RC, Kelly RA, Mitch WE. Mechanisms for defects in muscle protein metabolism in rats with chronic uremia: The influence of metabolic acidosis. J Clin Invest 79: 1099-1103, 1987.
24. McCurdy CE, Davidson RT, Cartee GD. Calorie restriction increases the ratio of phosphatidylinositol 3-kinase catalytic to regulatory subunits in rat skeletal muscle. Am J Physiol Endocrinol Metab 288: E996-E1001, 2005.
25. Meireles CL, Price SR, Pereira AML, Carvalhaes JTA, Mitch WE. Nutrition and chronic renal failure in rats: what is an optimal dietary protein? J Am Soc Nephrol 10: 2367-2373, 1999.
26. Mitch WE, Bailey JL, Wang X, Jurkovitz C, Newby D, Price SR. Evaluation of signals activating ubiquitin-proteasome proteolysis in a model of muscle wasting. Am J Physiol Cell Physiol 276: C1132-C1138, 1999.
27. Nellis MM, Doering CB, Kasinski A, Danner DJ. Insulin increases branched-chain α-ketoacid dehydrogenase kinase expression in Clone 9 rat cells. Am J Physiol Endocrinol Metab 283: E853-E860, 2002.
28. Price SR, Wang X, Bailey JL. Tissue-specific response of branched-chain α-ketoacid dehydrogenase activity in metabolic acidosis. J Am Soc Nephrol 9: 1892-1898, 1998.
29. Saad MJ, Folli F, Kahn JA, Kahn CR. Modulation of insulin receptor, insulin receptor substrate-1 and phoshatidylinositol 3-kinase in liver and muscle of dexamethasone-treated rats. J Clin Invest 92: 2065-2072, 1993.
30. Shen W, Brown NS, Finn PF, Dice JF, Franch HA. Akt and Mammalian target of rapamycin regulate separate systems of proteolysis in renal tubular cells. J Am Soc Nephrol 17: 2414-2423, 2006.
31. Singleton JR, Baker BL, Thorburn A. Dexamethasone inhibits insulinlike growth factor signaling and potentiates myoblast apoptosis. Endocrinology 141: 2945-2950, 2000.
32. Ueki K, Fruman DA, Brachmann SM, Tseng YH, Cantley LC, Kahn CR. Molecular balance between the regulatory and catalytic subunits of phosphoinositide 3-kinase regulates cell signaling and survival. Mol Cell Biol 22: 965-977, 2002.
33. Ueki K, Fruman DA, Yballe CM, Fassaur M, Klein J, Asano T, Cantley LC, Kahn CR. Positive and negative roles of p85alpha and p85beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling. J Biol Chem 278: 48453-48466, 2003.
34. Wang X, Chinsky JM, Costeas PA, Price SR. Acidification and glucocorticoids independently regulate branched-chain α-ketoacid dehydrogenase subunit genes. Am J Physiol Cell Physiol 280: C1176-C1183, 2001.
35. Wang X, Jurkovitz C, Price SR. Regulation of branched-chain ketoacid dehydrogenase flux by extracellular pH and glucocorticoids. Am J Physiol Cell Physiol 272: C2031-C2036, 1997.
36. Wang X, Price SR. Differential regulation of branched-chain α-ketoacid dehydrogenase kinase expression by glucocorticoids and acidification in LLC-PK1-GR101 cells. Am J Physiol Renal Physiol 286: F504-F508, 2004.