Pitfall of an internal control plasmid: Response of Renilla luciferase (pRL-TK) plasmid to dihydro-testosterone and dexamethasone

BioTechniques

Volumn 29, Issue 4, 2000, Pages 782-784

  Ibrahim, N M ^a   Marinovic, A C ^a   Price, S R ^a   Young, L G ^a   Frohlich, O ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANDROSTANOLONE; DEXAMETHASONE; LUCIFERASE; THYMIDINE KINASE;

ANIMAL CELL; ARTICLE; ASSAY; CELL LINE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; GENETIC TRANSFECTION; HORMONE RESPONSIVE ELEMENT; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION;

ANIMALIA; CERCOPITHECUS AETHIOPS; RENILLA; RENILLA LUCIFERASE;

EID: 0033793393     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00294st04     Document Type: Article

References (9)

1. Androgen and glucocorticoid receptor heterodimer formation. A possible mechanism for mutual inhibition of transcriptional activity
2. Testosterone and 5 α-dihydrotestosterone interact differently with the androgen receptor to enhance transcription of the MMTV-CAT reporter gene
3. A pitfall of using a second plasmid to determine transfection efficiency
4. Functional activities of the A and B forms of the human androgen receptor in response to androgen receptor agonists and antagonists
5. Luciferase reporter gene vectors for analysis of promoters and enhancers
6. Response elements of the androgen-regulated C3 gene
7. Androgen receptor antagonist versus agonist activities of the fungicide vinclozolin relative to hydroxyflutamide
8. The chemistry of bioluminescent reporter assays
9. HE2/EP2, an androgen-dependent protein from the epididymis of the chimpanzee, Pan troglodytes