Identification, characterization, epitope mapping, and three-dimensional modeling of the α-subunit of β-conglycinin of soybean, a potential allergen for young pigs

Journal of Agricultural and Food Chemistry

Volumn 55, Issue 10, 2007, Pages 4014-4020

  Fu, Chunjiang ^a   Jez, Joseph M ^b   Kerley, Monty S ^a   Allee, Gary L ^a   Krishnan, Hari B ^a  

^a UNIVERSITY OF MISSOURI   (United States)

^b DONALD DANFORTH PLANT SCIENCE CENTER   (United States)

Author keywords

conglycinin; Allergens; Epitope mapping; Pig; Soybean meal; Three dimensional structure

Indexed keywords

ALLERGEN; BETA CONGLYCININ PROTEIN, GLYCINE MAX; BETA-CONGLYCININ PROTEIN, GLYCINE MAX; GLOBULIN; PROTEIN SUBUNIT; SOYBEAN PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL; ANIMAL DISEASE; CHEMICAL STRUCTURE; CHEMISTRY; CONFERENCE PAPER; EPITOPE MAPPING; FOOD ALLERGY; IMMUNOLOGY; MOLECULAR GENETICS; SOYBEAN; SWINE; SWINE DISEASE;

ALLERGENS; AMINO ACID SEQUENCE; ANIMALS; EPITOPE MAPPING; FOOD HYPERSENSITIVITY; GLOBULINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN SUBUNITS; SOYBEAN PROTEINS; SOYBEANS; SWINE; SWINE DISEASES;

GLYCINE MAX; SUIDAE;

EID: 34249904970     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf070211o     Document Type: Conference Paper

References (51)

1. Cromwell, G. L. Soybean meal - The "gold standard"; The Farmer's Pride, 1999; https://www.uky.edu/Ag/AnimalSciences/pubs/soybeanmeal- thegolfstandard.PDF.
2. Pedersen, H. E. Allergenicity of soybean proteins. In Vegetable Protein Utilization in Human Foods and Animal Feedstuffs; Applewhite, T. H., Ed.; American Oil Chemists Society: Champaign, 1988; pp 204-211.
3. Zeece, M. G.; Beardslee, T. A.; Markwell, J.; Sarath, G. Identification of an IgE-binding region in soybean acidic glycinin GI. Food Agric. Immunol. 1999, 11, 83-90.
4. Heppel, L. M. J. W.; Sissons, J. W.; Pedersen, H. E. A comparison of the antigenicity of soya-bean-based infant formulas. Br. J. Nutr. 1987, 58, 393-403.
5. Burks, A. W., Jr.; Brooks, J. R.; Sampson, H. A. Allergenicity of major component proteins of soybean determined by enzyme-linked immunosorbent assay (ELISA) and immunoblotting in children with atopic dermatitis and positive soy challenges. J. Allergy Clin. Immunol. 1988, 81, 1135-1142.
6. Helm, R. M.; Cockrell, G.; Connaughton, C.; Sampson, H. A.; Bannon, G. A.; Beilinson, V.; Livingstone, D.; Nielsen, N. C.; Burks, A. W. A soybean G2 glycinin allergen 1. Identification and characterization. Int. Arch. Allergy Immunol. 2000, 123, 205-212.
7. Helm, R. M.; Cockrell, G.; Connaughton, C.; Sampson, H. A.; Bannon, G. A.; Beilinson, V.; Nielsen, N. C.; Burks, A. W. A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling. Int. Arch. Allergy Immunol. 2000, 123, 213-219.
8. Cásky, I.; Fekete, S. Soybean: Feed quality and safety. Part 1: Biologically active components - A review. Acta Vet. Hung. 2004, 52, 299-313.
9. Xiang, P.; Haas, E. J.; Zeece, M. G.; Markwell, J.; Sarath, G. C-terminal 23 kDa polypeptide of soybean Gly m Bd 28 K is a potential allergen. Planta 2004, 220, 56-63.
10. Helm, R. M. Joint FAO/WHA expert consultation on foods derived from biotechnology. Topic 5: Stability of known allergens (digestive and heat stability). Biotech. 01/07, 2001; http://www.fao.org/es/esn/food/pdf/bi07al.pdf.
11. Cásky, I.; Fekete, S. Soybean: Feed quality and safety part 2: Pathology of soybean feeding in animals - A review. Acta Vet. Hung. 2004, 52, 315-326.
12. Helm, R. M. Sensitization and allergic response and intervention therapy in animal models. J. AOAC Int. 2004, 87, 1441-1447.
13. Wilson, A. D.; Stokes, C. R.; Boume, F. J. Effect of age on absorption and immune response to weaning or introduction of novel dietary antigens in pigs. Res. Vet. Sci. 1989, 46, 180-186.
14. Li, D. F.; Nelssen, J. L.; Reddy, P. G.; Blecha, F.; Hancock, J. D.; Allee, G. L.; Goodband, R. D.; Klemm, R. D. Transient hypersensitivity to soyabean meal in the early-weaned pig. J. Anim. Sci. 1990, 68, 1790-1799.
15. Li, D. F.; Nelssen, J. L.; Reddy, P. G.; Blecha, F.; Klemm, R. D.; Giesting, D. W.; Hancock, J. D.; Allee, G. L.; Goodband, R. D. Measuring suitability of soybean products for early-weaned pigs with immunological criteria. J. Anim. Sci. 1991, 69, 3299-3307.
16. Drėau, D.; Lallės, J. P. Contribution to the study of gut hypersensitivity reactions to soybean proteins in preruminant calves and early-weaned piglets. Livestock Prod. Sci. 1999, 60, 209-218.
17. Salgado, P.; Freire, J. P. B.; Ferreira, R. B.; Teixeira, A.; Bento, O.; Abreu, M. C.; Toullec, R.; Lallės, J. P. Immunodetection of legume proteins resistant to small intestine digestion in weaned piglets. J. Sci. Food Agric. 2003, 83, 1571-1580.
18. Miller, B. G.; Newby, T. J.; Stokes, C. R.; Bourne, F. J. Influence of diet on post weaning malabsorption and diarrhoea in the pig. Res. Vet. Sci. 1984, 36, 187-193.
19. Lallės, J. P.; Salmon, H. Effects of dietary antigens on health, performance and immune system of pigs at weaning. In Proceedings of the 6th International Symposium on Digestive Physiology in Pigs; Souffrant, W. B., Hagemeister, H., Eds.; EAAP: Germany, 1994; pp 295-307.
20. NRC. Nutrient Requirements of Swine, 10th ed.; National Academy Press: Washington, DC, 1998.
21. Laemmli, U. K. Cleavage of structure proteins during assembly of the head of bacteriophage. Nature 1970, 227, 680-685.
22. Krishnan, H. B.; Natarajan, S. S.; Mahmoud, A. A.; Bennett, J. O.; Krishnan, A, H.; Prasad, B. N. Assessment of indigenous Nepalese soybean as a potential germplasms resource for improvement of protein in North American cultivars. J. Agric. Food Chem. 2006, 54, 5489-5497.
23. Astwood, J. D.; Leach, J. N.; Fuchs, R. L. Stability of food allergens to digestion in vitro. Nat. Biotechnol. 1996, 14, 1269-1273.
24. Maruyama, Y.; Maruyama, N.; Mikami, B.; Utsumi, S. Structure of the core region of the soybean β-conglycinin α′-subunit. Acta. Crystallogr. D 2004, 60, 289-297.
25. Schwede, T.; Kopp, J.; Guex, N.; Peitsch, M. C. Swiss-Model: An automated protein homology-modeling server. Nucleic Acids Res. 2003, 31, 3381-3385.
26. Laskowski, R. A.; MacArthur, M. W.; Moss, D. S.; Thornton, J. M. Procheck: A program to check the stereochemical quality of protein structures. J. Appl. Crystallogr. 1993, 26, 283-291.
27. Krishnan, H. B. Biochemistry and molecular biology of soybean seed storage protein. J. New Seeds 2000, 2, 1-25.
28. Fötisch, K.; Vieths, S. N- and O-linked oligosaccharides of allergenic glycoproteins. Glycoconjugate J. 2001, 18, 373-390.
29. Maruyama, N.; Katsube, T.; Wada, Y.; Oh, M. H.; Barba de la Rosa, A. P.; Okuda, E.; Nakagawa, S.; Utsumi, S. The roles of the N-linked glycans and extension regions of soybean β-conglycinin in folding, assembly and structural features. Eur. J. Biochem. 1998, 258, 854-862.
30. Thanh, V. H.; Shibasaki, K. Beta-conglycinin from soybean proteins. Isolation and immunological and physicochemical properties of the monomelic forms. Biochim. Biophys. Acta 1977, 490, 370-384.
31. Salgado, P.; Freire, J.; Ferreira, R.; Seabra, M.; Teixeira, A.; Toullec, R.; Lallės, J. Legume proteins of the vicilin family are more immunogenic than those of the legumin family in weaned piglets. Food Agric. Immunol. 2002, 14, 51-63.
32. Tryphonas, H.; George, A.; Elizabeth, V.; Genevieve, B. Animal models to detect allergenicity to foods and genetically modified products: Workshop summary. Environ. Health Perspect. 2003, 111, 221-222.
33. Helm, R. M.; Furuta, G. T.; Stanley, J. S.; Ye, J.; Cockrell, G.; Connaughton, C.; Simpson, P.; Bannon, G. A.; Burks, A. W. A neonatal swine model for peanut allergy. J. Allergy Clin. Immunol. 2002, 109, 136-142.
34. Helm, R. M.; Ermel, R. W.; Frick, O. L. Nonmurine animal models of food allergy. Environ. Health Perspect. 2003, 111, 239-244.
35. Awazuhara, H.; Kawai, H.; Maruchi, N. Major allergens in soybean and clinical significance of IgG4 antibodies investigated by IgE- and IgG4-immunoblotting with plasma from soybean-sensitive patients. Clin. Exp. Allergy 1997, 27, 325-332.
36. Magni, C.; Ballabio, C.; Restani, P.; Sironi, E.; Scarafoni, A.; Poiesi, C.; Duranti, M. Two-dimensional electrophoresis and western-blotting analyses with anti Ara h 3 basic subunit IgG evidence the cross-reacting polypeptides of Arachis hypogaea, Glycine max, and Lupinus albus seed proteomes. J. Agric. Food Chem. 2005, 53, 2275-2281.
37. Harada, J. J.; Barker, S. J.; Goldberg, R. B. Soybean β-conglycinin genes are clustered in several DNA regions and regulatedby transcriptional and posttranscriptional processes. Plant Cell 1989, 1, 415-425.
38. Xiang, P.; Beardslee, T. A.; Zeece, M. G.; Markwell, J.; Sarath, G. Identification and analysis of a conserved immunoglobulin E binding epitope in soybean G1a and G2a and peanut Ara h 3 glycinins. Arch. Biochem. Biophys. 2002, 408, 51-57.
39. Burks, A. W.; Shin, D.; Cockrell, G.; Stanley, J. S.; Helm, R. M.; Bannon, G. A. Mapping and mutational analysis of the IgE-binding epitopes on Ara h I, a legume vicilin protein and a major allergen in peanut hypersensitivity. Eur. J. Biochem. 1997, 245, 334-339.
40. Beardslee, T. A.; Zeece, M. G.; Sarath, G.; Markwell, J. P. Soybean glycinin G1 acidic chain shares IgE epitopes with peanut allergen Ara h 3. Int. Arch. Allergy Immunol. 2000, 123, 299-307.
41. Ogawa, A.; Samoto, M.; Takahashi, K. Soybean allergens and hypoallergenic soybean products. J. Nutr. Sci Vitaminol. 2000, 46, 271-279.
42. Herman, E. M.; Helm, R.; Jung, R.; Kinney, A. Genetic modification removes an immunodominant soybean allergen. Plant Physiol. 2003, 132, 36-43.
43. Samoto, M.; Takahashi, K.; Fukuda, Y.; Nakamura, S.; Kawamura, Y. Substantially complete removeal of the 34kDa allergenic soybean protein, Gly m Bd 30 K, from soy milk of a mutant lacking the α- and α′- subunits of conglycinin. Biosci., Biotechnol., Biochem. 1996, 60, 1911-1913.
44. Samoto, M.; Fukuda, Y. Y.; Takahashi, K.; Heimori, M.; Tsuji, H.; Ogawa, T.; Kawamura, Y. Substantially complete removal of three major allergenic soybean proteins (Gly m Bd 30 K, Gly m Bd 28 K and the alpha-subunit of conglycinin) from soy protein by using mutant soybean, Tohoku 124. Biosci., Biotechnol., Biochem. 1997, 61, 2148-2150.
45. Joseph, L. M.; Hymowitz, T.; Schmidt, M. A.; Herman, E. M. Evaluation of Glycine germplasms for nulls of the immunodominant allergen P34/Gly m Bd 30k. Crop Sci. 2006, 46, 1755-1763.
46. Takahashi, K.; Banba, H.; Kikuchi, A.; Ito, M.; Nakamura, S. An induced mutant line lacking the α-subunit of β-conglycinin in soybean [Glycine max (L.) Merrill]. Breed. Sci. 1994, 44, 65-66, 221-222.
47. Borchers, A. T.; Hackman, R. M.; Keen, C. L.; Stern, J. S.; Gershwin, M. E. Complementary medicine: a review of immunomodulatory effects of Chinese herbal medicines. Am. J. Clin. Nutr. 1997, 66, 1303-1312.
48. Kobayashi, S.; Watanabe, J.; Kawabata, J.; Fukushi, E.; Shinmoto, H. 2004. A novel method for producing a foodstuff from defatted black sesame seed that inhibits allergen absorption. Biosci., Biotechnol., Biochem. 2004, 68, 300-305.
49. Bernhisel-Broadbent, J.; Sampson, H. A. Cross-allergenicity in the legume botanical family in children with food hypersensitivity. J. Allergy Clin. Immunol. 1989, 83, 435-440.
50. Stanley, J. S.; Helm, R. M.; Ponnappan, U.; Sampson, H. A.; Burks, A. W. Allergen homologues as desensitizing reagents. J. Allergy Clin. Immunol. 2003, 111, S355-S440, Abstr. 828.
51. Buzen, F. R. Modification of the IgE-binding epitopes of Ara h 1 protein, a major peanut allergen, and its effect on IgE-binding, trimer formation, and clinical indicators of peanut allergy. Thesis, UAMS Library W4Ar48t, B988m, Little Rock, AR, 2002.