Molecular basis for thermal properties of Streptomyces thermovulgaris fumarase C hinge at hydrophilic amino acids R163, E170 and S347

Applied Microbiology and Biotechnology

Volumn 75, Issue 2, 2007, Pages 329-335

  Lin, Wenjie ^a   Chan, Maurice ^a   Goh, Liuh Ling ^a   Sim, Tiow Suan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Fumarase C; Hydrophilic residues; Site directed mutagenesis; Streptomyces; Thermostability

Indexed keywords

FUMARASE C; HYDROPHILIC RESIDUES; SITE-DIRECTED MUTAGENESIS; STREPTOMYCES; THERMOSTABILITY;

AMINO ACIDS; HIGH TEMPERATURE EFFECTS; MOLECULAR INTERACTIONS; MUTAGENS; THERMODYNAMIC STABILITY;

ENZYMES;

AMINO ACID; AMINO ACID E170; AMINO ACID R163; AMINO ACID S347; BACTERIAL ENZYME; BACTERIAL PROTEIN; FUMARASE C; MUTANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID; CATALYSIS; ENZYME; GENE EXPRESSION; MUTAGENICITY; PROTEIN; RECOMBINATION; TEMPERATURE PROFILE; THERMOPHILIC BACTERIUM;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME STABILITY; GENETIC DIFFERENCE; HIGH TEMPERATURE; HYDROPHILICITY; LOW TEMPERATURE; MESOPHILIC BACTERIUM; MOLECULAR BIOLOGY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; STREPTOMYCES; STREPTOMYCES THERMOVULGARIS; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

AMINO ACIDS; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; FUMARATE HYDRATASE; HEAT; HYDROGEN; INDUSTRIAL MICROBIOLOGY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; SEQUENCE ANALYSIS, DNA; STREPTOMYCES; STREPTOMYCES COELICOLOR;

STREPTOMYCES; STREPTOMYCES COELICOLOR; STREPTOMYCES THERMOVULGARIS;

EID: 34249889849     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0822-7     Document Type: Article

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