Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy

Biochemical Journal

Volumn 372, Issue 1, 2003, Pages 33-40

  Calleja, Véronique ^a,b   Ameer Beg, Simon M ^b   Vojnovic, Borivoj ^b   Woscholski, Rudiger ^c   Downward, Julian ^a   Larijani, Banafshé ^a  

^a IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^b MOUNT VERNON HOSPITAL   (United Kingdom)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

AGC kinases; Conformational change; Dark yellow fluorescent protein (YFPdark); Enhanced acceptor fluorescence; Fluorescence resonance energy transfer (FRET); Protein kinase B (PKB) Akt

Indexed keywords

CELLS; CONFORMATIONS; ENERGY TRANSFER; FLUORESCENCE; IMAGING TECHNIQUES; PHYSIOLOGY; PROTEINS; RESONANCE;

CONFORMATIONAL CHANGES;

BIOCHEMISTRY;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PLATELET DERIVED GROWTH FACTOR; PROTEIN KINASE B; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CONFORMATIONAL TRANSITION; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE MICROSCOPY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; STEREOSPECIFICITY;

ANIMALS; BACTERIAL PROTEINS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PROTEIN CONFORMATION; PROTEIN-SERINE-THREONINE KINASES; PROTEINS; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-AKT; RECOMBINANT FUSION PROTEINS;

ANIMALIA;

EID: 0038630916     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030358     Document Type: Article

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