Fluorescence lifetime measurements in confocal microscopy of neurons labeled with multiple fluorophores

Nature Biotechnology

Volumn 15, Issue 4, 1997, Pages 373-377

  Brismar, Hjalmar ^a   Ulfhake, Brun ^b  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CYANINE DYE; RHODAMINE;

AMINO ACID METABOLISM; ARTICLE; CONFOCAL MICROSCOPY; FLUORESCENCE; ISOTOPE LABELING; NERVE CELL; NERVE ENDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION;

ANIMALS; BIOTECHNOLOGY; CALCITONIN GENE-RELATED PEPTIDE; CARBOCYANINES; COMPUTER SIMULATION; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; GANGLIA, SPINAL; MICROSCOPY, CONFOCAL; NEURONS, AFFERENT; RATS; RATS, SPRAGUE-DAWLEY; RHODAMINES; SEROTONIN; SOMATOSTATIN; SPINAL CORD; SYNAPTOPHYSIN; XANTHENES;

EID: 0030998583     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0497-373     Document Type: Article

References (31)

1. Wessendorf, MW. 1900. Characterization and use of multi-color fluorescence microscopic technique, in Analysis of neuronal microcircuits and synaptic Interaction. Bjrklund, A., Hkfett, T., Woutertood, FG., and van den Pol, AN. (eds). Elsevier Publishing, Amsterdam.
2. Wessendorf, M.W. and Brelje, T.C. 1993. Multicolor fluorescence microscopy using the laser-scanning confocal microscope. Neuro. Protocote 2121.
3. Mossberg, K., Arvideson, U, and Ulfhake, B. 1990. Computerized quantification of immunofluorescence-labeled axon terminals and analysis of co-localization of neurochemicals in axon terminals with confocal scanning laser microscope. J. Histochem. Cytochem. 38:179.
4. Lakowicz, J.R, 1983. Principles of fluorescence spectroscopy. Plenum Press, New York.
5. Åslund, N. and Cartsson, K. 1994, Simultaneous life-time imaging of two fluorophores using a confocal laser microscope. IS & T/SPIE International Symposium on Electronic Imaging, Three-dimensional Microscopy: Image Acquisition and Processing. SPIE 2184, Proceedings.
6. Buurman, E.P., Sanders, R, Draaijer, A., Genitsen, HC van Veen, J.J.F., Houpt, RM., et al. 1992. Fluorescence lifetime Imaging using a confocal laser scanning microscope. Scanning 14:155.
7. Sasald, K., Koshioka, M., and Masuhara, H. 1991. Three-dimenslonal space-and time-resolved fluorescence spectroscopy. Applied Spectroscopy 45:1041.
8. Ghiggino, K.P., Harris, M.R., and Spizziri, P.G. 1992. Fluorescence lifetime measurements using a novel fiber-optic laser seaming confocal microscope. Rev Sci Instrum. 63:2939.
9. Brismar, H., Trepte, O., and Ulfhake, B. 1995. Spectra and fluorescence lifetimes of Ussamine Rhodamine, tetramethyl rhodamine isothiocyanate, Texas Red, and Cyanlne 3.18 ftuocoprxxes, and influences of some environmental factors recorded with a confocal laser scanning microscope. J. Histochem. Cytochem. 43:699.
10. Todd, AJ. and Spike, R C. 1993. The localization of classical transmitters and neuropeptides within neurons in laminae I-III of the mammalian spinal dorsal horn. Progr. Neurobiology 41:608–645.
11. Wims, W.D., Jr. and Coggeshall, R. E. 1991. Sensory mechanisms of the spinalcord, 2nd ed Plenum Press, New York.
12. Xu Zhang. 1994. Messenger plasticity in primary sensory neurons following peripheral nerve injury. Thesis, Karollnska Institutet.
13. Köllner, M. and Wolfrum, J. 1992. How many photons are necessary for fluorescence-lifetime measurements? Chem. Phys. Left 200:199.
14. Fiorijn, R.J., Slats, J., Tanke, H.J., and Raap, A. K. 1995. Analysis of antifadeing reagents for fluorescence microscopy. Cytometry 19:177–182.
15. Oida, T., Sako, Y, and Kusumi, A 1993. Fluorescence lifetime imaging microscopy (flimscopy). Biophys J. 64:676.
16. Morgan, C.Q., Mitchell, AC, and Murray, J.G. 1991. Prospects for confocal Imaging based on nanosecond fluorescence decay time. J. Microscopy 165:49.
17. Lakowicz, J.R., Szmacinski, K, Nowaczyk, K., Barndt, K.W., and Johnson, M.L 1992. Fluorescence lifetime imaging. Analytical Biochem. 202:316.
18. Spencer, R.D. and Weber, Q. 1969. Measurements of subnanosecond fluorescence lifetimes with a cross-correlation phase fluorometer. Ann. NY Acad. Sci. 158:361.
19. Gratton, E. and Limkeman, M. 1983. A continuously variable frequency cross correlation phase fluorometer with picosecond resolution. Biophys. J. 44:315.
20. Gadella, T.W.J., Jovin, T.M., and Clegg, R.M. 1993. Fluorescence lifetime imaging microscopy (FUM): spatial resolution of microstructures on the nanosecond time scale. Biophys. Chem. 48:221.
21. Lakowicz, J.R., Laczko, G., Cherek, H., Gratton, E., and Limkeman, M. 1984. Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data. Biophys. J. 46:463.
22. Weber, G. 1981. Resolution of the fluorescence lifetimes In a heterogenous system by phase and modulation measurements. J. Phys. Chem. 86:949.
23. Gratton, E., Limkeman, M., Lakowicz, J.R., Maliwal, B.R, Cherek, H, Laczko, G. 1984. Resolution of mixtures of fluorophores using variable-frequency phase and modulation data. Biophys. J. 46:479.
24. Ju, G., Hkfelt, T, Brodln, E., Fahrenkrug, J., Fischer, JA, Frey, P., et al. 1987. Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-like immunoreactivie ganglion cells. Cell TISS. Res. 247:417–431.
25. Cameron, AA, Leah, J.D., and Snow, P.J. 1988. The coexistence of neuropeptides In feline sensory neurons. Neurosci. 27:969–979.
26. Hunt, S.R 1983. pp. 53–64 in Cytochemistry of the spinel chord. Chemical Neuroanatomy. Emson, P.C. (ed.). Raven Press, New York.
27. Tuchscherer, M.M. and Seybold, V.S. 1989. A quantitative study of the coexistence of peptides in varicosities within the superficial laminae of the dorsal rxm of the rat cord. J. Neurosci. 8:195–205.
28. Arvidsson, U., et al. 1990. 5-Hycbotiyptamine, substance P, and thyrotropin-releasing hormone in the adult cat spinal cord segment L7: immunohistochemical and chemical studies. Synapse 6:237–270.
29. O'Connor, D.V. and Philips, D. 1984. Time-correlated single photon counting. Academic Press, London.
30. Åstund, N. and Cartsson, K. 1987. Confocal imaging for 3-0 digital microscopy. Appl. Opt 26:3232.
31. Marquardt, D.W. 1963. An algorithm for least-squares estimation of nonlinear parameters. J. Soc. Indust Appl. Math. 11:431.