Creation of Rhizopus oryzae lipase having a unique oxyanion hole by combinatorial mutagenesis in the lid domain

Applied Microbiology and Biotechnology

Volumn 68, Issue 6, 2005, Pages 779-785

  Shiraga, Seizaburo ^a   Ishiguro, Masaji ^b   Fukami, Harukazu ^c   Nakao, Masahiro ^c   Ueda, Mitsuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b SUNTORY INSTITUTE FOR BIOORGANIC RESEARCH   (Japan)

^c SUNTORY LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CELLS; FATTY ACIDS; MUTAGENESIS; NEGATIVE IONS; YEAST;

COMBINATORIAL LIBRARIES; NON-POLAR AMINO ACIDS; YEAST CELL SURFACE;

ENZYMES;

ALANINE; AMINO ACID; ASPARTIC ACID; FATTY ACID ESTER; FLUORESCEIN; FUNGAL ENZYME; ISOLEUCINE; PHENYLALANINE; RHIZOPUS ORYZAE LIPASE; SERINE; SOYBEAN OIL; THREONINE; UNCLASSIFIED DRUG;

ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CELL CLONING; CELL MUTANT; CELL SURFACE; COMBINATORIAL LIBRARY; COMPUTER MODEL; ENZYME ACTIVITY; ENZYME SPECIFICITY; HYDROPHOBICITY; MUTAGENESIS; NONHUMAN; RHIZOPUS ORYZAE; YEAST CELL;

AMINO ACID SEQUENCE; ANIONS; CELL MEMBRANE; COMBINATORIAL CHEMISTRY TECHNIQUES; LIPASE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RHIZOPUS; SUBSTRATE SPECIFICITY;

GLYCINE MAX; RHIZOPUS ORYZAE;

EID: 27544481402     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-1935-0     Document Type: Article

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