δ-Integration of endo/exo-glucanase and β-glucosidase genes into the yeast chromosomes for direct conversion of cellulose to ethanol

Enzyme and Microbial Technology

Volumn 25, Issue 1-2, 1999, Pages 23-30

  Cho, Kwang Myung ^a   Yoo, Young Je ^a   Kang, Hyen Sam ^a  

^a Seoul National University   (South Korea)

Author keywords

Glucosidase; Integration; Cellulose; Endo glucanase; Ethanol; Exo glucanase; Saccharomyces cerevisiae

Indexed keywords

CELLULOSE; ETHANOL; FERMENTATION; SACCHARIFICATION;

ENDOGLUCANASE; EXOGLUCANASE; GLUCOSIDASE; SACCROMYCES CEREVISIAE;

ENZYMES;

ALCOHOL; BETA GLUCOSIDASE; CELLULOSE; GLUCAN SYNTHASE;

ARTICLE; BACTERIUM CULTURE; CHROMOSOME; DRUG MANUFACTURE; ELECTROPORATION; ENZYME ACTIVITY; GENE TRANSFER; PROTEIN EXPRESSION; RECOMBINANT DNA TECHNOLOGY; RECOMBINANT GENE; SACCHAROMYCES CEREVISIAE; TECHNIQUE;

SACCHAROMYCES CEREVISIAE;

EID: 0032999856     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(99)00011-3     Document Type: Article

References (32)

1. Takagi M, Abe S, Suzuki S, Emert, GH, Yata N. A method for production of alcohol directly from cellulose using cellulase and yeast. In: Bioconversion Symposium Proceedings. Dehli, India: IIT, 1977. p. 551-71.
2. Wright J.D., Wyman C.E., Grohmann K. Simultaneous saccharification and fermentation of lignocellulose. Appl Biochem Biotechnol. 18:1988;75-90.
3. Van Arsdell J.N., Kwok S., Schweickart V.L., Ladner M.B., Gelfand D.H., Innis M.A. Cloning, characterization and expression in Saccharomyces cerevisiae of endoglucanase I from Trichoderma reesei. Biotechnology (NY). 5:1987;60-64.
4. Penttila M.E., Andre L., Saloheimo M., Lehtovaara P., Knowles J.K.C. Expression of two T. reesei endoglucanases in the yeast Saccharomyces cerevisiae. Yeast. 3:(3):1987;175-185.
5. Penttila M.E., Andre L., Lehtovaara P., Bailey M., Teeri T.T., Knowles J.K.C. Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae. Gene. 63:(1):1988;103-112.
6. Cummings C., Fowler T. Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae. Curr Genet. 29:(3):1996;227-233.
7. Raynal A., Guerineau M. Cloning and expression of the structural gene for β-glucosidase of Kluyveromyces fragilis in Escherichia coli and Saccharomyces cerevisiae. Mol Gen Genet. 195:(1-2):1984;108-115.
8. Benitez J., Silva A., Vazquez R., Noa M.D., Hollenberg C.P. Secretion and glycosylation of Clostridium thermocellum endoglucanase A encoded by the celA gene in Saccharomyces cerevisiae. Yeast. 5:1989;99-306.
9. Adam A.C., Rubio-Texeira M., Polaina J. Induced expression of bacterial β-glucosidase activity in Saccharomyces. Yeast. 11:(5):1995;395-406.
10. Bitter G.A., Egan K.M., Koski E.R., Jones M.D., Elliott S.G., Giffin J.C. Expression and secretion vectors for yeast. Methods Enzymol. 153:1987;516-544.
11. Rose M.D. Isolation of genes by complementation in yeast. Methods Enzymol. 152:1987;481-504.
12. Denis C.L., Drouin E.E. Meiotic instability of tandemly iterated plasmid sequences in the yeast chromosome. Curr Genet. 12:(6):1987;399-403.
13. Lopes T.S., Klootwijk J., Veenstra A.E., van der Aar P.C., van Heerikhuizen H.A., Planta R.J. High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae a new vector for high-level expression . Gene. 79:(2):1989;199-206.
14. Boeke J.D., Hua X., Fink G.R. A general method for the chromosomal amplification of genes in yeast. Science. 239:(4837):1988;280-282.
15. Jacobs E., Rutgers T., Voet P., Dewerchin M., Cabezen T., de Wilde M. Simultaneous synthesis and assembly of various hepatitis B surface proteins in Saccharomyces cerevisiae. Gene. 80:(2):1989;279-291.
16. Sakai A., Shimizu Y., Hishinuma F. Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast transposon Ty. Appl Microbiol Biotechnol. 33:(3):1990;302-306.
17. Sakai A., Ozawa A., Higashizaki T., Shimizu Y., Hishinuma F. Enhanced secretion of human nerve growth factor from Saccharomyces cerevisiae using an advanced δ-integration system. Biotechnology (NY). 9:(12):1991;1382-1385.
18. Mochizuki D., Miyahara K., Matsuzaki H., Hatano T., Fukui S., Miyakawa T. Overexpression and secretion of cellulolytic enzymes by δ-sequence-mediated multicopy integration of heterologous DNA sequences into the chromosomes of Saccharomyces cerevisiae. J Ferm Bioeng. 77:(5):1994;468-473.
19. Wang X., Wang Z., Da Silva N.A. G418 selection and stability of cloned genes integrated at the chromosomal δ-sequences of Saccharomyces cerevisiae. Biotechnol Bioeng. 49:1996;45-51.
20. Lee F.W.K., Da Silva N.A. Sequential δ-integration for the regulated insertion of cloned genes in Saccharomyces. Biotechnol Prog. 13:(4):1997;368-373.
21. Lee F.W.K., Da Silva N.A. Ty1-mediated integration of expression cassettes host strain effect, stability, and product synthesis . Biotechnol Prog. 12:(4):1996;548-554.
22. Lee F.W.K., Da Silva N.A. Application of Ty1 for cloned gene insertion amplification of a large regulated expression cassette in Saccharomyces cerevisiae . Appl Microbiol Biotechnol. 44:(5):1996;620-623.
23. Cameron J.R., Loh E.Y., Davis R.W. Evidence for transposition of dispersed repetitive DNA families in yeast. Cell. 16:(4):1979;739-761.
24. Dujon B. The yeast genome project what did we learn? Trend Genet. 12:(7):1996;263-270.
25. Hinnen A., Hicks J.B., Fink G.R. Transformation of yeast. Proc Natl Acad Sci USA. 75:(4):1978;1929-1933.
26. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 153:(1):1983;163-168.
27. Hasimoto H., Yamada Y., Kimura A. A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA. Appl Microbiol Biotechnol. 21:1985;336-339.
28. Meilhoc E., Masson J.M., Teissie J. High efficiency transformation of intact yeast cells by electric field pulses. Biotechnology (NY). 8:(3):1990;223-227.
29. Han S.J., Yoo Y.J., Kang H.S. Characterization of bifunctional cellulase and its structural gene. J Biol Chem. 270:(43):1995;26012-26019.
30. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning a laboratory manual, 2nd ed . 1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
31. Sherman F., Fink G.R., Hicks J.B. Method in yeast genetics. 1986;Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
32. Miller G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem. 31:1959;420-428.