Development of a simple and robust assay to screen for inhibitors of sphingosine kinases

Assay and Drug Development Technologies

Volumn 5, Issue 2, 2007, Pages 215-223

  Togame, Hiroko ^a,b   Dodo, Reiko ^a,b   Inaoka, Tetsuya ^a,c   Reinemer, Peter ^a,d  

^a BAYER YAKUHIN LTD   (Japan)

^b SHIONOGI AND CO LTD   (Japan)

^c TAKEDA PHARMACEUTICAL COMPANY LIMITED   (Japan)

^d PROTEROS BIOSTRUCTURES GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; N,N DIMETHYLSPHINGOSINE; PHOSPHORUS 33; SPHINGOSINE; SPHINGOSINE 1 PHOSPHATE; SPHINGOSINE KINASE;

ARTICLE; AUTOMATION; CONTROLLED STUDY; DEVICE; DRUG SCREENING; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; ENZYME MECHANISM; EXTRACTION; IN VITRO STUDY; NONHUMAN; SEPARATION TECHNIQUE; THIN LAYER CHROMATOGRAPHY;

ADENOSINE TRIPHOSPHATE; AUTOMATION; DRUG EVALUATION, PRECLINICAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; ESCHERICHIA COLI; KINETICS; LYSOPHOSPHOLIPIDS; PHOSPHORYLATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); RECOMBINANT PROTEINS; SPHINGOSINE;

EID: 34247624514     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2006.049     Document Type: Article

References (27)

1. Taha TA, Hannun YA, Obeid LM: Sphingosine kinase: biochemical and cellular regulation and role in disease. J Biochem Mol Biol 2006;39:113-131.
2. Kohama T, Olivera A, Edsall L, Nagiec MM, Dickson R, Spiegel S: Molecular cloning and functional characterization of murine sphingosine kinase. J Biol Chem 1998;273:23722-23728.
3. Liu H, Sugiura M, Nava VE, Edsal LC, Kono K, Poulton S, et al.: Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. J Biol Chem 2000;275:19513-19520.
4. Spiegel S, Milstein S: Sphingosine-1-phosphate: an enigmatic signalling lipid. Nat Rev Mol Cell Biol 2003;4:397-407.
5. Pyne S, Pyne NJ: Sphingosine 1-phosphate signalling and termination at lipid phosphate receptors. Biochim Biophys Acta 2002;1582:121-131.
6. Baumruker T, Prieschl EE: Sphingolipids and the regulation of the immune response. Semin Immunol 2002;14: 57-63.
7. Hla T, Lee MJ, Ancellin N, Paik JH, Kluk MJ: Lysophospholipids-receptor revelations. Science 2001;294:1875-1878.
8. Spiegel S: Sphingosine 1-phosphate: a prototype of a new class of second messengers. J Leukoc Biol 1999;65:341-344.
9. Pyne S, Pyne NJ: Sphingosine 1-phosphate signaling in mammalian cells. Biochem J 2000;349:385-402.
10. Hannun YA, Luberto C, Argraves KM: Enzymes of sphingolipid metabolism: from modular to integrative signaling. Biochemistry 2001;40:4893-4903.
11. Maceyka M, Payne SG, Milstien S, Spiegel S: Sphingosine kinase, sphingosine-1-phosphate, and apoptosis. Biochim Biophys Acta 2002;1585:193-201.
12. Pitson SM, Moretti PAB, Zebol JR, Xia P, Gamble JR, Vadas MA, et al.: Expression of a catalytically inactive sphingosine kinase mutant blocks agonist-induced sphingosine kinase activation: a dominant-negative sphingosine kinase. J Biol Chem 2000;275:33945-33950.
13. Olivera A, Barlow KD Spiegel S: Assaying sphingosine kinase activity. Methods Enzymol 2000;311:215-223.
14. Louie DD, Kisic A, Schoroepfer GJ: Sphingolipid base metabolism: partial purification and properties of sphinganine kinase of brain. J Biol Chem 1976;251:4557-4564.
15. Buehrer BM, Bell RM: Inhibition of sphingosine kinase in vitro and in platelets. J Biol Chem 1992;267:3154-3159.
16. Caligan TB, Peters K, Ou J, Wang E, Saba J, Merrill AH: A high-performance liquid chromatographic method to measure sphingosine 1-phosphate and related compounds from sphingosine kinase assays and other biological samples. Anal Biochem 2000;281:36-44.
17. Ruwisch L, Schafer-Korting M, Kleuser B: An improved high-performance liquid chromatography method for the determination of sphingosine 1-phosphate in complex biological materials. Naunyn Schmiedebergs Arch Pharmacol 2001;363:358-363.
18. Min J-K, Yoo H-S, Lee E-Y, Lee W-J, Lee Y-M: Simultaneous quantitative analysis of sphingoid base 1-phosphates in biological samples by o-phthalaldehyde precolumn derivatization after dephosphorylation with alkaline phosphatase. Anal Biochem 2002;303:167-175.
19. Billich A, Ettmayer P: Fluorescence-based assay of sphingosine kinases. Anal Biochem 2004;326:114-119.
20. Roberts JL, Moretti PAB, Darrow AL, Derian CK, Vadas MA, Pitson SM: An assay for sphingosine kinase activity using biotinylated sphingosine and streptavidin-coated membranes. Anal Biochem 2004;331:122-129.
21. Gregorius K, Mouritsen S, Eisner H: Hydrocoating: a new method for coupling biomolecules to solid phases. J Immunol Methods 1995;181:65-73.
22. Zhang JH, Chung TDY, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4: 67-73.
23. Pitson SM, D'Andrea RJ, Vandeleur L, Moretti PAB, Xia P, Gamble JR, et al.: Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem J 2000;350:429-441.
24. Melendez AJ, Carlos-Dias E, Gosink M, Allen JM, Takacs L: Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 2000; 251:19-26.
25. Olivera A, Kohama T, Tu Z, Milstien S, Spiegel S: Purification and characterization of rat kidney sphingosine kinase. J Biol Chem 1998;273:12576-12583.
26. Gijsbers S, Asselberghs S, Herdewijn P, Van Veldhoven PP: 1-o-Hexadecyl-2-desoxy-2-amino-sn-glycerol, a substrate for human sphingosine kinase. Biochim Biophys Acta 2002;1580:1-8.
27. Nava VE, Lacana E, Poulton S, Liu H, Sugiura M, Kono K, et al.: Functional characterization of human sphingosine kinase-1. FEBS Lett 2000;473;81-84.