Human sphingosine kinase: Purification, molecular cloning and characterization of the native and recombinant enzymes

Biochemical Journal

Volumn 350, Issue 2, 2000, Pages 429-441

  Pitson, S M ^a   D'Andrea, R J ^a   Vandeleur, L ^a   Moretti, P A B ^a   Xia, P ^a   Gamble, J R ^a   Vadas, M A ^a   Wattenberg, B W ^a  

^a INSTITUTE OF MEDICAL AND VETERINARY SCIENCE   (Australia)

Author keywords

Enzyme activation; Lipid kinase; Second messenger; Sphingosine 1 phosphate

Indexed keywords

ISOENZYME; RECOMBINANT ENZYME; SPHINGOSINE 1 PHOSPHATE; SPHINGOSINE KINASE;

ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME CONFORMATION; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; HUMAN TISSUE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLACENTA; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMMONIUM SULFATE; CALMODULIN; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTHELIUM, VASCULAR; ENZYME ACTIVATION; ESCHERICHIA COLI; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; PHOSPHOLIPIDS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLACENTA; PROTEIN CONFORMATION; PROTEIN ISOFORMS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; UMBILICAL CORD;

EID: 0034283195     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3500429     Document Type: Article

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