Characterization of the proapoptotic intracellular mechanisms induced by a toxic conformer of the recombinant human prion protein fragment 90-231

Annals of the New York Academy of Sciences

Volumn 1090, Issue , 2006, Pages 276-291

  Villa, Valentina ^a   Corsaro, Alessandro ^a   Thellung, Stefano ^a   Paludi, Domenico ^b   Chiovitti, Katia ^c   Venezia, Valentina ^a   Nizzari, Mario ^a   Russo, Claudio ^d   Schettini, Gennaro ^a   Aceto, Antonio ^c   Florio, Tullio ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF TERAMO   (Italy)

^c UNIVERSITY OF CHIETI   (Italy)

^d UNIVERSITY OF MOLISE   (Italy)

Author keywords

Apoptosis; Caspases; p38 MAP kinase; Prion protein

Indexed keywords

4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; BRAIN PROTEIN; CASPASE 3; DNA FRAGMENT; ISOPROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; MITOGEN ACTIVATED PROTEIN KINASE P38 INHIBITOR; OLIGOMER; PRION PROTEIN; RECOMBINANT PROTEIN; CASPASE;

AMINO ACID SEQUENCE; APOPTOSIS; BETA SHEET; CELL DEATH; CELL SURVIVAL; CONFERENCE PAPER; CONTROLLED STUDY; DENATURATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; NEUROTOXICITY; PHYSICAL CHEMISTRY; PROTEIN CONFORMATION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; WESTERN BLOTTING; ARTICLE; CELL LINE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; METABOLISM; PRION;

ANIMALIA;

APOPTOSIS; CASPASES; CELL LINE; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PRIONS; PROTEIN ISOFORMS; RECOMBINANT PROTEINS;

EID: 34247553753     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1196/annals.1378.030     Document Type: Conference Paper

References (32)

1. PRUSINER, S.B. 2001. Shattuck lecture-neurodegenerative diseases and prions. N. Engl. J. Med. 344: 1516-1526.
2. PRUSINER, S.B., M.R. SCOTT, S.J. DEARMOND & F.E. COHEN. 1998. Prion protein biology. Cell 93: 337-348.
3. AGUZZI, A. & M. POLYMENIDOU. 2004. Mammalian prion biology: one century of evolving concepts. Cell 116: 313-327.
4. GALLO, M., D. PALUDI, D.O. CICERO, et al. 2005. Identification of a conserved N-capping box important for the structural autonomy of the prion alpha 3-helix: the disease associated D202N mutation destabilizes the helical conformation. Int. J. Immunopathol. Pharmacol. 18: 95-112.
5. CHIESA, R. & D.A. HARRIS 2001. Prion diseases: what is the neurotoxic molecule? Neurobiol. Dis. 8: 743-763.
6. CORSARO, A., S. THELLUNG, C. RUSSO, et al. 2002. Expression in E. coli and purification of recombinant fragments of wild type and mutant human prion protein. Neurochem. Int. 41: 55-63.
7. THELLUNG, S., V. VILLA, A. CORSARO, et al. 2002. p38 MAP kinase mediates the cell death induced by PrP106-126 in the SH-SY5Y neuroblastoma cells. Neurobiol. Dis. 9: 69-81.
8. PRUSINER, S.B. 1998. Prions. Proc. Natl. Acad. Sci. USA 95: 13363-13383.
9. MORILLAS, M., D.L. VANIK & W.K. SUREWICZ. 2001. On the mechanism of alphahelix to beta-sheet transition in the recombinant prion protein. Biochemistry 40: 6982-6987.
10. FLORIO, T., D. PALUDI, V. VILLA, et al. 2003. Contribution of two conserved glycine residues to fibrillogenesis of the 106-126 prion protein fragment. Evidence that a soluble variant of the 106-126 peptide is neurotoxic. J. Neurochem. 85: 62-72.
11. CORSARO, A., S. THELLUNG, V. VILLA, et al. 2003. Prion protein fragment 106-126 induces a p38 MAP kinase-dependent apoptosis in SH-SY5Y neuroblastoma cells independently from the amyloid fibril formation. Ann. N. Y. Acad. Sci. 1010: 610-622.
12. O'DONOVAN, C.N., D. TOBIN & T.G. COTTER. 2001. Prion protein fragment PrP-(106-126) induces apoptosis via mitochondrial disruption in human neuronal SH-SY5Y cells. J. Biol. Chem. 276: 43516-43523.
13. HETZ, C., M. RUSSELAKIS-CARNEIRO, K. MAUNDRELL, et al. 2003. Caspase-12 and endoplasmic reticulum stress mediate neurotoxicity of pathological prion protein. Embo. J. 22: 5435-5445.
14. FORLONI, G., N. ANGERETTI, R. CHIESA, et al. 1993. Neurotoxicity of a prion protein fragment. Nature 362: 543-546.
15. HENGARTNER, M.O. 2000. The biochemistry of apoptosis. Nature 407: 770-776.
16. MIELKE, K. & T. HERDEGEN. 2000. JNK and p38 stresskinases-degenerative effectors of signal-transduction-cascades in the nervous system. Prog. Neurobiol. 61: 45-60.
17. ZOU, W.Q., S. CAPELLARI, P. PARCHI, et al. 2003. Identification of novel proteinase K-resistant C-terminal fragments of PrP in Creutzfeldt-Jakob disease. J. Biol. Chem. 278: 40429-40436.
18. FORLONI, G., R. DEL BO, N. ANGERETTI, et al. 1994. A neurotoxic prion protein fragment induces rat astroglial proliferation and hypertrophy. Eur. J. Neurosci. 6: 1415-1422.
19. THELLUNG, S., T. FLORIO, A. CORSARO, et al. 2000. Intracellular mechanisms mediating the neuronal death and astrogliosis induced by the prion protein fragment 106-126. Int. J. Dev. Neurosci. 18: 481-492.
20. THELLUNG, S., T. FLORIO, V. VILLA, et al. 2000. Apoptotic cell death and impairment of L-type voltage-sensitive calcium channel activity in rat cerebellar granule cells treated with the prion protein fragment 106-126. Neurobiol. Dis. 7: 299-309.
21. BROWN, D.R., B. SCHMIDT & H.A. KRETZSCHMAR. 1996. Role of microglia and host prion protein in neurotoxicity of a prion protein fragment. Nature 380: 345-347.
22. FLORIO, T., S. THELLUNG, C. AMICO, et al. 1998. Prion protein fragment 106-126 induces apoptotic cell death and impairment of L-type voltage-sensitive calcium channel activity in the GH3 cell line. J. Neurosci. Res. 54: 341-352.
23. JACKSON, G.S., A.F. HILL, C. JOSEPH, et al. 1999. Multiple folding pathways for heterologously expressed human prion protein. Biochim. Biophys. Acta 1431: 1-13.
24. SWIETNICKI, W., M. MORILLAS, S.G. CHEN, et al. 2000. Aggregation and fibrillization of the recombinant human prion protein huPrP90-231. Biochemistry 39: 424-431.
25. BROWN, D.R. 2000. PrPSc-like prion protein peptide inhibits the function of cellular prion protein. Biochem. J. 352: 511-518.
26. DANIELS, M., G.M. CEREGHETTI & D.R. BROWN. 2001. Toxicity of novel C-terminal prion protein fragments and peptides harbouring disease-related C-terminal mutations. Eur. J. Biochem. 268: 6155-6164.
27. PILLOT, T., B. DROUET, M. PINCON-RAYMOND, et al. 2000. A nonfibrillar form of the fusogenic prion protein fragment [118-135] induces apoptotic cell death in rat cortical neurons. J. Neurochem. 75: 2298-2308.
28. GHATAN, S., S. LARNER, Y. KINOSHITA, et al. 2000. p38 MAP kinase mediates bax translocation in nitric oxide-induced apoptosis in neurons. J. Cell Biol. 150: 335-347.
29. MORIYA, R., T. UEHARA & Y. NOMURA. 2000. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484: 253-260.
30. HAN, J., J.D. LEE, L. BIBBS & R.J. ULEVITCH. 1994. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 265: 808-811.
31. KUMMER, J.L., P.K. RAO & K.A. HEIDENREICH. 1997. Apoptosis induced by withdrawal of trophic factors is mediated by p38 mitogen-activated protein kinase. J. Biol. Chem. 272: 20490-20494.
32. KAWASAKI, H., T. MOROOKA, S. SHIMOHAMA, et al. 1997. Activation and involvement of p38 mitogen-activated protein kinase in glutamate-induced apoptosis in rat cerebellar granule cells. J. Biol. Chem. 272: 18518-18521.