The conserved carboxy-terminal region of the ammonia channel AmtB plays a critical role in channel function

Molecular Membrane Biology

Volumn 24, Issue 2, 2007, Pages 161-171

  Severi, Emmanuele ^a   Javelle, Arnaud ^b   Merrick, Mike ^c  

^a UNIVERSITY OF YORK   (United Kingdom)

^b PAUL SCHERRER INSTITUTE   (Switzerland)

^c JOHN INNES CENTRE   (United Kingdom)

Author keywords

Ammonia channel; Ammonium transport; AmtB

Indexed keywords

AMMONIUM TRANSPORT PROTEIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

AMMONIA FORMATION; ARCHAEBACTERIUM; ARTICLE; BACTERIUM; BIOINFORMATICS; CARBOXY TERMINAL SEQUENCE; CHANNEL GATING; ESCHERICHIA COLI; FUNGUS; GENETIC CONSERVATION; PHENOTYPE; PLANT; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

CATION TRANSPORT PROTEINS; CONSERVED SEQUENCE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MODELS, MOLECULAR; MUTATION; NUCLEOTIDYLTRANSFERASES; PII NITROGEN REGULATORY PROTEINS; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN STRUCTURE, TERTIARY; QUATERNARY AMMONIUM COMPOUNDS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FUNGI;

EID: 34247328584     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.1080/09687860601129420     Document Type: Article

References (35)

1. Andrade SL, Dickmanns A, Ficner R, Einsle O. 2005. Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus. Proc Natl Acad Sci USA 102:14994-14999.
2. Biswas K, Morschhauser J. 2005. The Mep2p ammonium permease controls nitrogen starvation-induced filamentous growth in Candida albicans. Mol Microbiol 56:649-669.
3. Blakey D, Leech A, Thomas GH, Coutts G, Findlay K, Merrick M. 2002. Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry. Biochem J 364:527-535.
4. Casadaban MJ, Cohen SN. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol 138:179-207.
5. Conroy MJ, Jamieson SJ, Blakey D, Kaufmann T, Engel A, Fotiadis D, Merrick M, Bullough PA. 2004. Electron and atomic force microscopy of the trimeric ammonium transporter AmtB. EMBO Reports 5:1153-1158.
6. Coutts G, Thomas G, Blakey D, Merrick M. 2002. Membrane sequestration of the signal transduction protein GlnK by the ammonium transporter AmtB. EMBO J 21:536-545.
7. Durand A, Merrick M. 2006. In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate. J Biol Chem 281:29558-29567.
8. Emsley P, Cowtan K. 2004. Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60:2126-2132.
9. Facey SJ, Kuhn A. 2003. The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC. Eur J Biochem 270:1724-1734.
10. Javelle A, Merrick M. 2005. Complex formation between AmtB and GlnK: an ancestral role in prokaryotic nitrogen control. Biochem Soc Trans 33:174-176.
11. Javelle A, Severi E, Thornton J, Merrick M. 2004. Ammonium sensing in E.coli: the role of the ammonium transporter AmtB and AmtB-GlnK complex formation. J Biol Chem 279:8530-8538.
12. Javelle A, Thomas G, Marini AM, Kramer R, Merrick M. 2005. In vivo functional characterisation of the E. coli ammonium channel AmtB: evidence for metabolic coupling of AmtB to glutamine synthetase. Biochem J 390:215-222.
13. 14C]methylamine and other highly permeant solutes. Anal Biochem 135:475-478.
14. Khademi S, O'Connell J, III, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM. 2004. Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 Å. Science 305:1587-1594.
15. Lin Y, Cao Z, Mo Y. 2006. Molecular dynamics simulations on the Escherichia coli ammonia channel protein AmtB: Mechanism of ammonia/ammonium transport. J Am Chem Soc 128:10876-10884.
16. Liu XY, Matherly LH. 2002. Analysis of membrane topology of the human reduced folate carrier protein by hemagglutinin epitope insertion and scanning glycosylation insertion mutagenesis. Biochim Biophys Acta 1564:333-342.
17. Liu Z, Peng J, Mo R, Hui C, Huang CH. 2001. Rh Type B glycoprotein is a new member of the Rh superfamily and a putative ammonia transporter in mammals. J Biol Chem 276:1424-1433.
18. Lorenz MC, Heitman J. 1998. The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae. EMBO J 17:1236-1247.
19. +-uniporters. J Biol Chem 278:45603-45610.
20. Marini A-M, Andre B. 2000. In vivo N-glycosylation of the Mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus. Mol Microbiol 38:552-564.
21. Marini A-M, Matassi G, Raynal V, Andre B, Cartron JP, Cherif-Zahar B. 2000a. The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet 26:341-344.
22. Marini A-M, Soussi-Boudekou S, Vissers S, Andre B. 1997. A family of ammonium transporters in Saccharomyces cerevisiae. Mol Cell Biol 17:4282-4293.
23. Marini A-M, Springael JY, Frommer WB, Andre B. 2000b. Crosstalk between ammonium transporters in yeast and interference by the soybean SAT1 protein. Mol Microbiol 35:378-385.
24. Monahan BJ, Unkles SE, Tsing IT, Kinghorn JR, Hynes MJ, Davis MA. 2002. Mutation and functional analysis of the Aspergillus nidulans ammonium permease MeaA and evidence for interaction with itself and MepA. Fungal Genet Biol 36:35-46.
25. Schwede T, Kopp J, Guex N, Peitsch MC. 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31:3381-3385.
26. Simons RW, Houman F, Kleckner N. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85-96.
27. Smith DG, Garcia-Pedrajas MD, Gold SE, Perlin MH. 2003. Isolation and characterization from pathogenic fungi of genes encoding ammonium permeases and their roles in dimorphism. Mol Microbiol 50:259-275.
28. 3: proteins from hyperthermophiles complement an Escherichia coli mutant. J Bacteriol 184:3396-3400.
29. Thomas G, Coutts G, Merrick M. 2000a. The glnKamtB operon: a conserved gene pair in prokaryotes. Trends Genet 16:11-14.
30. Thomas GH, Mullins JG, Merrick M. 2000b. Membrane topology of the Mep/Amt family of ammonium transporters. Mol Microbiol 37:331-344.
31. Thornton J, Blakey D, Scanlon E, Merrick M. 2006. The ammonia channel protein AmtB from Escherichia coli is a polytopic membrane protein with a cleavable signal peptide. FEMS Microbiol Lett 258:114-120.
32. Van Nuland A, Vandormael P, Donaton M, Alenquer M, Lourenco A, Quintino E, Versele M, Thevelein JM. 2006. Ammonium permease-based sensing mechanism for rapid ammonium activation of the protein kinase A pathway in yeast. Mol Microbiol 59:1485-1505.
33. von Wirén N, Merrick M. 2004. Regulation and function of ammonium carriers in bacteria, fungi and plants. Trends Curr Genetics 9:95-120.
34. Winkler FK. 2006. Amt/MEP/Rh proteins conduct ammonia. Pflugers Arch 451:701-707.
35. Zheng L, Kostrewa D, Bernèche S, Winkler FK, Li X-D. 2004. The mechanism of ammonia transport based on the crystal structure of AmtB of E. coli. Proc Natl Acad Sci USA 101:17090-17095.