Mechanism of ammonia transport by Amt/MEP/Rh: Structure of AmtB at 135 Å

Science

Volumn 305, Issue 5690, 2004, Pages 1587-1594

  Khademi, Shahram ^a   O'Connell III, Joseph ^a   Remis, Jonathan ^a   Robles Colmenares, Yaneth ^a   Miercke, Larry J W ^a   Stroud, Robert M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROPHOBICITY; MEMBRANES; PROTEINS;

HYDROPHOBIC CHANNEL; METHYL AMMONIA;

AMMONIA;

AMMONIA; AMMONIUM DERIVATIVE; AMTB PROTEIN; CARRIER PROTEIN; HISTIDINE; MEP PROTEIN; METHYLAMMONIA; PERMEASE; RHESUS ANTIGEN; UNCLASSIFIED DRUG; AMTB PROTEIN, E COLI; CATION TRANSPORT PROTEIN; ESCHERICHIA COLI PROTEIN; LIPOSOME; QUATERNARY AMMONIUM DERIVATIVE; WATER;

CHEMISTRY;

AMINO TERMINAL SEQUENCE; AQUIFEX AEOLICUS; ARTICLE; BINDING SITE; CELL PH; ESCHERICHIA COLI; HYDROPHOBICITY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MEMBRANE CHANNEL; MEMBRANE VESICLE; NITROGEN METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; AMINO ACID SEQUENCE; BLOOD GROUP RHESUS SYSTEM; CELL MEMBRANE; CELL MEMBRANE POTENTIAL; CHEMICAL STRUCTURE; CHEMISTRY; CRYSTALLIZATION; GENETICS; HYDROGEN BOND; METABOLISM; MOLECULAR GENETICS; PH; PROTEIN CONFORMATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; TRANSPORT AT THE CELLULAR LEVEL; X RAY CRYSTALLOGRAPHY;

AQUIFEX AEOLICUS; ESCHERICHIA COLI; STAPHYLOCOCCUS PHAGE 3A;

AMINO ACID SEQUENCE; AMMONIA; BINDING SITES; BIOLOGICAL TRANSPORT; CATION TRANSPORT PROTEINS; CELL MEMBRANE; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; HYDROPHOBICITY; LIPOSOMES; MEMBRANE POTENTIALS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; QUATERNARY AMMONIUM COMPOUNDS; RH-HR BLOOD-GROUP SYSTEM; SEQUENCE ALIGNMENT; WATER;

EID: 4644220261     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1101952     Document Type: Article

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38. We thank J. Finer-Moore for her enlightened counsel. J. Holten for his assistance at the Advanced Light Source (ALS) beamline 83.1, and M. Mann for assistance in molecular biology. E. Soupene and S. Kustu suggested structure determination of an AmtB, and we thank them for providing an Aqulfex amtB done pJES1331, a sotubilized cell pellet and partially purified Aquifex protein. Supported by NIH grant no. GM24485 (R.M.S.). Coordinates (accession codes apo, 1U77; Am, 1U7C; and MA, 1U7C) and structure factors (accession codes: apo, RCSB023331; Am, RCSB023340; and MA RCSB023336) of the structures have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank.