Correlation between functional and binding activities of designer zinc-finger proteins

Biochemical Journal

Volumn 403, Issue 1, 2007, Pages 177-182

  Kang, Jong Seok ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Designer transcription factor; Repression; Transcriptional regulation; Zinc finger protein; Zinc finger protein 268 (Zif268)

Indexed keywords

DESIGNER TRANSCRIPTION FACTORS; REPRESSION; TRANSCRIPTIONAL REGULATION; ZINC-FINGER PROTEIN; ZINC-FINGER PROTEIN 268 (ZIF268);

BIOASSAY; BIOCHEMISTRY; CORRELATION METHODS; ZINC;

PROTEINS;

GALACTOSIDASE; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN; ZINC FINGER PROTEIN 268;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; DISSOCIATION CONSTANT; DNA BINDING; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FUNCTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

CELL LINE; EARLY GROWTH RESPONSE PROTEIN 1; GENES, REPORTER; HUMANS; KIDNEY; KINETICS; LUCIFERASES; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 34147132352     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20061644     Document Type: Article

References (15)

1. Elrod-Erickson, M., Rould, M. A., Nekludova, L. and Pabo, C. O. (1996) Zif268 protein-DNA complex refined at 1.6 Å: a model system for understanding zinc finger-DNA interactions. Structure 4, 1171-1180
2. Jamieson, A. C., Miller, J. C. and Pabo, C. O. (2003) Drug discovery with engineered zinc-finger proteins. Nat. Rev. Drug Discovery 2, 361-368
3. 2 zinc finger proteins. Annu. Rev. Biochem. 70, 313-340
4. Sepp, A. and Choo, Y. (2005) Cell-free selection of zinc finger DNA-binding proteins using in vitro compartmentalization. J. Mol. Biol. 354, 212-219
5. Rebar, E. J., Greisman, H. A. and Pabo, C. O. (1996) Phage display methods for selecting zinc finger proteins with novel DNA-binding specificities. Methods Enzymol. 267, 129-149
6. Bae, K. H., Kwon, Y. D., Shin, H. C., Hwang, M. S., Ryu, E. H., Park, K. S., Yang, H. Y., Lee, D. K., Lee, Y., Park, J. et al. (2003) Human zinc fingers as building blocks in the construction of artificial transcription factors. Nat. Biotechnol. 21, 275-280
7. Ihara, H., Mie, M., Funabashi, H., Takahashi, F., Sawasaki, T., Endo, Y. and Kobatake, E. (2006) In vitro selection of zinc finger DNA-binding proteins through ribosome display. Biochem. Biophys. Res. Commun. 345, 1149-1154
8. Kim, J. S. and Pabo, C. O. (1998) Getting a handhold on DNA: design of poly-zinc finger proteins with femtomolar dissociation constants. Proc. Natl. Acad. Sci. U.S.A. 95, 2812-2817
9. Liu, Q., Segal, D. J., Ghiara, J. B. and Barbas, 3rd, C. F. (1997) Design of polydactyl zinc-finger proteins for unique addressing within complex genomes. Proc. Natl. Acad. Sci. U.S.A. 94, 5525-5530
10. Kang, J. S. and Kim, J. S. (2000) Zinc finger proteins as designer transcription factors. J. Biol. Chem. 275, 8742-8748
11. Kim, J. S. and Pabo, C. O. (1997) Transcriptional repression by zinc finger peptides: exploring the potential for applications in gene therapy. J. Biol. Chem. 272, 29795-29800
12. Yaghmai, R. and Cutting, G. R. (2002) Optimized regulation of gene expression using artificial transcription factors. Mol. Ther. 5, 685-694
13. Liu, P. Q., Rebar, E. J., Zhang, L., Liu, Q., Jamieson, A. C., Liang, Y., Qi, H., Li, P. X., Chen, B., Mendel, M. C. et al. (2001) Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions: activation of vascular endothelial growth factor A. J. Biol. Chem. 276, 11323-11334
14. Guarente, L. (1983) Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol. 101, 181-191
15. Wolfe, S. A., Greisman, H. A., Ramm, E. I. and Pabo, C. O. (1999) Analysis of zinc fingers optimized via phage display: evaluating the utility of a recognition code. J. Mol. Biol. 285, 1917-1934