In vitro selection of zinc finger DNA-binding proteins through ribosome display

Biochemical and Biophysical Research Communications

Volumn 345, Issue 3, 2006, Pages 1149-1154

  Ihara, Hiroshi ^a   Mie, Masayasu ^a   Funabashi, Hisakage ^a   Takahashi, Fumio ^b   Sawasaki, Tatsuya ^b   Endo, Yaeta ^b   Kobatake, Eiry ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b EHIME UNIVERSITY   (Japan)

Author keywords

DNA binding protein; In vitro selection; Ribosome display; Zif268; Zinc finger

Indexed keywords

DNA BINDING PROTEIN; DNA FRAGMENT; MESSENGER RNA; STREPTAVIDIN; ZINC FINGER PROTEIN;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELL CLONE; CONTROLLED STUDY; DNA BINDING; DNA LIBRARY; GENE; GENE AMPLIFICATION; IN VITRO SELECTION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; RETICULOCYTE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RIBOSOME; RNA TRANSLATION; SEQUENCE ANALYSIS; ZIF268 GENE;

ANIMALS; BIOTINYLATION; CELL LINE, TUMOR; DNA PRIMERS; DNA-BINDING PROTEINS; EARLY GROWTH RESPONSE PROTEIN 1; GENE LIBRARY; GENETIC TECHNIQUES; MICE; MUTATION; PLASMIDS; RIBOSOMES; RNA, MESSENGER; ZINC FINGERS;

ORYCTOLAGUS CUNICULUS;

EID: 33646844421     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.05.029     Document Type: Article

References (16)

1. Pavletich N.P., and Pabo C.O. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A. Science 252 (1991) 809-817
2. Park K.S., Lee D.K., Lee H., Lee Y., Jang Y.S., Kim Y.H., Yang H.Y., Lee S.I., Seol W., and Kim J.S. Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors. Nat. Biotechnol. 21 (2003) 1208-1214
3. Blancafort P., Chen E.E., Gonzalez B., Bergquist S., Zijlsta A., Guthy D., Brachat A., Brakenhoff R.H., Quigley J.P., Erdmann D., and Barbas III C.F.. Genetic reprogramming of tumor cells by zinc finger transcription factors. Proc. Natl. Acad. Sci. USA 102 (2005) 11716-11721
4. McNamara A.R., Hurd P.J., Smith A.E., and Ford K.G. Characterization of site-biased DNA methyltransferases: specificity, affinity and subsite relationships. Nucleic Acids Res. 30 (2002) 3818-3830
5. Bibikova M., Carroll D., Segal D.J., Trautman J.K., Smith J., Kim Y.G., and Chandrasegaran S. Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol. Cell. Biol. 21 (2001) 289-297
6. Smith G.P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228 (1985) 1315-1317
7. Bartsevich V.V., and Juliano R.L. Regulation of the MDR1 gene by transcriptional repressors selected using peptide combinatorial libraries. Mol. Pharmacol. 58 (2000) 1-10
8. Joung J.K., Ramm E.I., and Pabo C.O. A bacterial two-hybrid selection system for studing protein-DNA and protein-protein interactions. Proc. Natl. Acad. Sci. USA 97 (2000) 7382-7387
9. Mattheakis L.C., Bhatt R.R., and Dower W.J. An in vitro polysome display system for identifying ligands from very large peptide libraries. Proc. Natl. Acad. Sci. USA 91 (1994) 9022-9026
10. Roberts R.W., and Szostak J.W. RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc. Natl. Acad. Sci. USA 94 (1997) 12297-12302
11. Tawfik D.S., and Griffiths A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16 (1998) 652-656
12. Takahashi F., Funabashi H., Mie M., Endo Y., Sawasaki T., Aizawa M., and Kobatake E. Activity-based in vitro selection of T4 DNA ligase. Biochem. Biophys. Res. Commun. 336 (2005) 987-993
13. Sawasaki T., Ogasawara T., Morishita R., and Endo Y. A cell-free protein synthesis system for high-throughput proteomics. Proc. Natl. Acad. Sci. USA 99 (2002) 14652-14657
14. Madin K., Sawasaki T., Ogasawara T., Morishita R., and Endo Y. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes. Proc. Natl. Acad. Sci. USA 97 (2000) 559-564
15. Takahashi F., Ebihara T., Mie M., Yanagida Y., Endo Y., Kobatake E., and Aizawa M. Ribosome display for selection of active dihydrofolate reductase mutants using immobilized methotrexate on agarose beads. FEBS Lett. 514 (2002) 106-110
16. Sepp A., and Choo Y. Cell-free selection of zinc finger DNA-binding proteins using in vitro compartmentalization. J. Mol. Biol. 354 (2005) 212-219