Side population analysis using a violet-excited cell-permeable DNA binding dye

Stem Cells

Volumn 25, Issue 4, 2007, Pages 1029-1036

  Telford, William G ^a   Bradford, Jolene ^b   Godfrey, William ^b   Robey, Robert W ^a   Bates, Susan E ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b MOLECULAR PROBES INC   (United States)

Author keywords

Bone marrow; Cord blood; DyeCycle; Hoechst 33342; Side population; Stem cell

Indexed keywords

FUMITREMORGIN C; HOE 33342;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CELL POPULATION; CONTROLLED STUDY; DNA BINDING; EFFUSION; FLOW CYTOMETRY; HEMATOPOIETIC CELL; HEMATOPOIETIC STEM CELL; IMMUNOPHENOTYPING; ION TRANSPORT; MALE; MOUSE; NONHUMAN; ULTRAVIOLET RADIATION;

ANIMALS; BENZIMIDAZOLES; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; DNA; FLOW CYTOMETRY; FLUORESCENT DYES; HEMATOPOIETIC STEM CELLS; HUMANS; LASERS; MICE; MICE, INBRED C57BL;

DROSOPHILA C VIRUS; MAMMALIA;

EID: 34147127558     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2006-0567     Document Type: Article

References (19)

1. Goodell MA, Brose K, Paradis G et al. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 1996;183:1797-1806.
2. Goodell MA, Rosenzweig M, Kim H et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nature Med 1997;3:1337-1345.
3. Goodell MA. Stem cell identification and sorting using the Hoeschst 33342 side population (SP). In: Robinson JP, Darzynkiewicz Z, Dean, PN et al, eds. Current Protocols in Cytometry. New York: John Wiley & Sons, 2001:9.18.1-9.18.xx.
4. Challen GA, Little MH. A side order of stem cells: The SP phenotype. STEM CELLS 2006;24:3-12.
5. Litman T, Brangi M, Hudson E et al. The multidrug-resistant phenotype associated with overexpression of the new ABC half-transporter, MXR (ABCG2). J Cell Sci 2000;113:2011-2021.
6. Zhou S, Schuetz JD, Bunting KD et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med 2001;7:1028-1034.
7. Scharenberg CW, Harkey MA, Torok-Storb B. The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hematopoietic progenitors. Blood 2002;99:507-512.
8. Uchida N, Leung FY, Eaves CJ. Liver and marrow of adult mdr-1a/1b(-/-) mice show normal generation, function, and multi-tissue trafficking of primitive hematopoietic cells. Exp Hematol 2002;30:862-869.
9. Kim M, Turnquist H, Jackson J et al. The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. Clin Cancer Res 2002;8:22-28.
10. Telford WG. Small lasers in flow cytometry. In: Hawley TS, Hawley RG, eds. Methods in Molecular Biology, Volume 263, Flow Cytometry Protocols, 2nd edition. London: Humana Press, 2004:399-418.
11. Telford WG. Analysis of UV-excited fluorochromes by flow cytometry using a near-UV laser diode. Cytometry A 2004;61:9-17.
12. Telford WG, Frolova EG. Discrimination of Hoechst side population in mouse bone marrow with violet and near-UV laser diodes. Cytometry A 2004;57:45-52.
13. Simpson C, Pearce DJ, Bonnet D et al. Out of the blue: A comparison of Hoechst side population (SP) analysis of murine bone marrow using 325, 363 and 407 nm excitation sources. J Immunol Methods 2006;310:171-181.
14. Eaker SS, Hawley TS, Ramezani A et al. Detection and enrichment of hematopoietic stem cells by side population phenotype. In: Hawley TS, Hawley RG, eds. Methods in Molecular Biology, Volume 263, Flow Cytometry Protocols, 2nd edition. London: Humana Press, 2004:399-418.
15. Kawanabe N, Murakami K, Takana-Yamamoto T. The presence of ABCG2-dependent side positive cells in human periodontal ligaments. Biochem Biophys Res Comm 2006;344:1278-1283.
16. Robey RW, Honjo Y, Morisaki K et al. Mutations at amino-acid 482 in the ABCG2 gene affect substrate and antagonist specificity. Br J Cancer 2003;89:1971-1978.
17. Telford W, Kapoor V, Jackson J et al. Violet laser diodes in flow cytometry: An update. Cytometry 2006;69:1153-1160.
18. Schwarz BA, Bhandoola A. Circulating hematopoietic progenitors with T lineage potential. Nat Immunol 2004;5:953-960.
19. Cabana R, Frolova EG, Thomas RA et al. The minimal instrumentation requirements for Hoechst side population analysis: Stem cell analysis on low-cost flow cytometry platforms. STEM CELLS 2006;24:2573-2581.