Immunogold labeling study of the distribution of GLUT-1 and GLUT-4 in cardiac tissue following stimulation by insulin or ischemia

American Journal of Physiology - Heart and Circulatory Physiology

Volumn 292, Issue 4, 2007, Pages

  Davey, Katherine A B ^a   Garlick, Pamela B ^a   Warley, Alice ^a   Southworth, Richard ^a,b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b GUY S HOSPITAL   (United Kingdom)

Author keywords

Glucose transporter; Immunogold electron microscopy

Indexed keywords

GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 4; INSULIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL MEMBRANE; CELL TYPE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; HEART LEFT VENTRICLE; HEART MUSCLE CELL; HEART MUSCLE FIBER MEMBRANE; HEART PERFUSION; HEART STIMULATION; IMMUNOGOLD LABELING; ISCHEMIA; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; TRANSVERSE TUBULAR SYSTEM; WESTERN BLOTTING;

ANIMALS; ANTIBODY SPECIFICITY; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; BUFFERS; CELL FRACTIONATION; GLUCOSE TRANSPORTER TYPE 1; GLUCOSE TRANSPORTER TYPE 4; HYPOGLYCEMIC AGENTS; IMMUNOHISTOCHEMISTRY; INSULIN; MALE; MICROSCOPY, IMMUNOELECTRON; MYOCARDIAL ISCHEMIA; MYOCARDIUM; MYOCYTES, CARDIAC; RATS; RATS, WISTAR; SARCOLEMMA;

EID: 34147109102     PISSN: 03636135     EISSN: 15221539     Source Type: Journal    
DOI: 10.1152/ajpheart.00663.2006     Document Type: Article

References (42)

1. Aledo JC, Lavoie L, Volchuk A, Keller SR, Klip A, Hundal HS. Identification and characterization of two distinct intracellular GLUT4 pools in rat skeletal muscle: evidence for an endosomal and an insulin-sensitive GLUT4 compartment. Biochem J 325: 727-732, 1997.
2. Alpert E, Gruzman A, Riahi Y, Blejter R, Aharoni P, Weisinger G, Eckel J, Kaiser N, Sasson S. Delayed autoregulation of glucose transport in vascular endothelial cells. Diabetologia 48: 752-755, 2005.
3. Askenasy N. Glycolysis protects sarcolemmal membrane integrity during total ischemia in the rat heart. Basic Res Cardiol 96: 612-622, 2001.
4. Bornemann A, Ploug T, Schmalbruch H. Subcellular localization of GLUT4 in nonstimulated and insulin-stimulated soleus muscle of rat. Diabetes 41: 215-221, 1992.
5. Brette F, Orchard C. T-tubule function in mammalian cardiac myocytes. Circ Res 92: 1182-1192, 2003.
6. Bryant NJ, Govers R, James DE. Regulated transport of the glucose transporter GLUT4. Nat Rev Mol Cell Biol 3: 267-277, 2002.
7. Doria-Medina CL, Lund DD, Pasley A, Sandra A, Sivitz WI. Immunolocalization of GLUT-1 glucose transporter in rat skeletal muscle and in normal and hypoxic cardiac tissue. Am J Physiol Endocrinol Metab 265: E454-E464, 1993.
8. Egert S, Nguyen N, Brosius FC 3rd, Schwaiger M. Effects of wortmannin on insulin- and ischemia-induced stimulation of GLUT4 translocation and FDG uptake in perfused rat hearts. Cardiovasc Res 35: 283-293, 1997.
9. Egert S, Nguyen N, Schwaiger M. Myocardial glucose transporter GLUT1: translocation induced by insulin and ischemia. J Mol Cell Cardiol 31: 1337-1344, 1999.
10. Farrell CL, Pardridge WM. Blood-brain barrier glucose transporter is asymmetrically distributed on brain capillary endothelial lumenal and ablumenal membranes: an electron microscopic immunogold study. Proc Natl Acad Sci USA 88: 5779-5783, 1991.
11. Fernandes R, Suzuki K, Kumagai AK. Inner blood-retinal barrier GLUT 1 in long-term diabetic rates and immunogold electron microscopic study. Invest Ophthalmol Vis Sci 44: 3150-3154, 2003.
12. Fischer Y, Thomas J, Sevilla L, Munoz P, Becker C, Holman G, Kozka IJ, Palacin M, Testar X, Kammermeier H, Zorzano A. Insulin-induced recruitment of glucose transporter 4 (GLUT4) and GLUT1 in isolated rat cardiac myocytes. J Biol Chem 272: 7085-7092, 1997.
13. Friedman JE, Dudek RW, Whitehead DS, Downes DL, Frisell WR, Caro JF, Dohm GL. Immunolocalization of glucose transporter GLUT4 within human skeletal muscle. Diabetes 40: 150-154, 1991.
14. Fuller W, Eaton P, Medina RA, Bell J, Shattock MJ. Differential centrifugation separates cardiac sarcolemmal and endosomal membranes from Langendorff-perfused rat hearts. Anal Biochem 293: 216-223, 2001.
15. Gathereole DV, Colling DJ, Skepper JN, Takagishi Y, Levi AJ, Severs NJ. Immunogold-labeled L-type calcium channels are clustered in the surface plasma membrane overlying junctional sarcoplasmic reticulum in guinea-pig lyocytes - implications for excitation-contraction coupling in cardiac muscle. J Mol Cell Cardiol 32: 1981-1994, 2000.
16. Griffiths G. Fine Structure Immunocytochemistry. New York: Springer-Verlag, 1993.
17. Kahn BB. Facilitative glucose transporters: regulatory mechanisms and dysregulation in diabetes. J Clin Invest 89: 1367-1374, 1992.
18. Kennergren C, Mantovani V, Strindberg L, Berglin E, Hamberger A, Lonnroth P. Myocardial interstitial glucose and lactate before, during, and after cardioplegic heart arrest. Am J Physiol Endocrinol Metab 284: E788-E794, 2003.
19. Kraegen EW, Sowden JA, Halstead MB, Clark PW, Rodnick KJ, Chisholm DJ, James DE. Glucose transporters and in vivo glucose uptake in skeletal and cardiac muscle: fasting, insulin stimulation and immunoisolation studies of GLUT1 and GLUT4. Biochem J 295: 287-293, 1993.
20. Kramer HF, Witezak CA, Fijii N, Jessen N, Taylor EB, Arnolds DE, Sakamoto K, Hirschmann MF, Goodyear LJ. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes 55: 2067-2076, 2006.
21. Kurth-Kraczek EJ, Hirshman MF, Goodyear LJ, Winder WW. 5′ AMP-activated protein kinase activation causes GLUT 4 translocation in skeletal muscle. Diabetes 48: 1667-1671, 1999.
22. Laemli UK. Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
23. Laybutt DR, Thompson AL, Cooney GJ, Kraegen EW. Selective chronic regulation of GLUT1 and GLUT4 content of insulin, glucose, and lipid in rat cardiac muscle in vivo. Am J Physiol Heart Circ Physiol 273: H1309-H1316, 1997.
24. Mann GE, Yudilevich DL, Sobrevia L. Regulation of amino acid and glucose transporters in endothelial and smooth muscle cells. Physiol Rev 83: 183-252, 2003.
25. Mayhew TM, Lucocq JM, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc 205: 153-164, 2001.
26. Medina RA, Southworth R, Fuller W, Garlick PB. Lactate-induced translocation of GLUT1 and GLUT4 is not mediated by the phosphatidylinositol-3- kinase pathway in the rat heart. Basic Res Cardiol 97: 168-176, 2002.
27. Rett K, Wicklmayr M, Dietze GJ, Häring HU. Insulin-induced glucose transporter (GLUT1 and GLUT4) translocation in cardiac muscle tissue is mimicked by bradykinin. Diabetes 45: S66-S69, 1996.
28. Russell R, Nguyen N, Mrus JM, Taegtmeyer H. Fasting and lactate unmask insulin responsiveness in the isolated working rat heart. Am J Physiol Endocrinol Metab 263: E556-E561, 1992.
29. Russell RR, Bergeron R, Shulman GI, Young LH. Translocation of myocardial GLUT-4 and increased glucose uptake through activation of AMPK by AICAR. Am J Physiol Heart Circ Physiol 277: H643-H649, 1999.
30. Slot JW, Geuze HJ, Gigengack S, James DE, Lienhard GE. Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat. Proc Natl Acad Sci USA 88: 7815-7819, 1991.
31. Southworth R, Dearling JLJ, Medina RA, Flynn AA, Pedley RB, Garlick PB. Dissociation of glucose tracer uptake and glucose transporter distribution in the regionally ischaemic isolated rat heart: application of a new autoradiographic technique. Eur J Nucl Med 29: 1334-1341, 2002.
32. Stanley WC, Recchia FA, Lopaschuk GD. Myocardial substrate metabolism in the normal and failing heart. Physiol Rev 85: 1093-1129, 2005.
33. Treebak JT, Glund S, Deshmukh A, Klein DK, Long YC, Jensen TE, Jørgsen SB, Viollet B, Anderson L, Neumann D, Walliman T, Richter EA, Chibalin AV, Zierdth JR, Wojtaszenski JFP. AMPK-mediated AS160 phosphorylation in skeletal muscle is dependent on AMPK catalytic and regulatory subunits. Diabetes 55: 2051-2058, 2006.
34. + exchanger are immediately active upon post-ischemic reperfusion in isolated rat hearts. J Mol Cell Cardiol 30: 337-348, 1998.
35. Wang PJ, Lloyd SG, Chatham JC. Impact of high glucose/high insulin and dichloroacetate treatment on carbohydrate oxidation and functional recovery after low-flow ischemia and reperfusion in the isolated perfused rat heart. Circulation 111: 2066-2072, 2005.
36. Wang W, Hansen PA, Marshall BA, Holloszy JO, Mueckler M. Insulin unmasks a COOH-terminal GLUT4 epitope and increases glucose transport across T-tubules in skeletal muscle. J Cell Biol 135: 415-460, 1996.
37. Wilson JE. Distinguishing the type I and type II isozymes of hexokinase: the need for a reexamination of past practice. Diabetes 47: 1544-1548, 1998.
38. Xia Y, Warshaw JB, Haddad GC. Effect of chronic hypoxia on glucose transporters in heart and skeletal muscle of immature and adult rats. Am J Physiol Regul Integr Comp Physiol 273: R1734-R1741, 1997.
39. Yoshihara T, Satoh M, Yamamura Y, Itoh H, Ishii T. Ultrastructural localization of glucose transporter 1 (GLUT 1) in guinea pig stria vascularis and vestibular dark cell areas: an immunogold study. Acta Otolaryngol (Stockh) 119: 336-340, 1999.
40. Young LH, Renfu Y, Russell R, Hu X, Caplan M, Ren J, Shulman GI, Sinusas AJ. Low-flow ischemia leads to translocation of canine heart GLUT4 and GLUT1 glucose transporters to the sarcolemma in vivo. Circulation 95: 415-422, 1997.
41. Young LH, Russell RR, Yin R, Caplan MJ, Ren J, Bergeron R, Shulman GI, Sinusas AJ. Regulation of myocardial glucose uptake and transport during ischemia and energetic stress. Am J Cardiol 83: 25H-30H, 1999.
42. Zierler K. Whole body glucose metabolism. Am J Physiol Endocrinol Metab 276: E409-E426, 1999.