Cloning and heterologous expression of bovine pyroglutamyl peptidase type-1 in Escherichia coli: Purification, biochemical and kinetic characterisation

Molecular and Cellular Biochemistry

Volumn 297, Issue 1-2, 2007, Pages 189-197

  Kilbane, Zelda ^a   Vaas, Paul Roman ^b   Cuív, Páraic Ó ^a   O'Connor, Brendan ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

^b DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

Characterisation; Cloning; Expressionin E. coli; Purification; Pyroglutamyl peptidase type 1

Indexed keywords

5 OXOPROLYL PEPTIDASE; ALANINE; CYSTEINE PROTEINASE; DIPEPTIDE; DITHIOTHREITOL; GLUTAMIC ACID; HISTIDINE; NICKEL; PROLINE; PROTIRELIN; TRIPEPTIDE; VALINE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BIOCHEMISTRY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; COW; CULTURE MEDIUM; ENZYME KINETICS; ESCHERICHIA COLI; GENETIC CODE; HETEROLOGOUS EXPRESSION; HUMAN; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CATTLE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; KINETICS; MOLECULAR SEQUENCE DATA; PYROGLUTAMYL-PEPTIDASE I; RECOMBINANT PROTEINS;

BOVINAE; ESCHERICHIA COLI;

EID: 33947430048     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-006-9346-9     Document Type: Article

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