Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis

Extremophiles

Volumn 4, Issue 5, 2000, Pages 297-303

  Singleton, Martin R ^a,c   Taylor, Stephen J C ^b   Parrat, Julian S ^b,d   Littlechild, Jennifer A ^a  

^a UNIVERSITY OF EXETER   (United Kingdom)

^b Chirotech Ltd   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

^d Peboc Division of Eastman Chemicals (UK) Ltd   (United Kingdom)

Author keywords

Biotransformations; Catalytic triad; Peptidase; Thermococcus; Thermostability

Indexed keywords

5 OXOPROLYL PEPTIDASE; ARCHAEAL PROTEIN; RECOMBINANT PROTEIN; SOLVENT;

AMINO ACID SEQUENCE; ARTICLE; BIOTRANSFORMATION; CHEMISTRY; DRUG EFFECT; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; TEMPERATURE; THERMOCOCCUS;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; BIOTRANSFORMATION; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PYROGLUTAMYL-PEPTIDASE I; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMOCOCCUS;

EID: 0034303174     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920070017     Document Type: Article

References (31)

1. Awadé A, Cleuziat P, Gonzales T, Robert-Baudouy J (1992) Characterization of the Pcp gene encoding the pyrrolidone carboxyl peptidase of Bacillus subtilis. FEBS Lett 305:67-73
2. Awadé AC, Cleuziat P, Gonzales T, Robert-Baudouy J (1994) Pyrrolidone carboxyl peptidase (Pcp): an enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptides and pGlu-proteins. Proteins Struct Funct Genet 20:34-51
3. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
4. Bundgaard H, Moss J (1989) Prodrug derivatives of thyrotropin-releasing hormone and other peptides. Biochem Soc Trans 17:947-949
5. Cleuziat P, Awade A, Robert-Baudouy J (1992) Molecular characterization of Pcp, the structural gene encoding the pyrrolidone carboxylyl peptidase from Streptococcus pyogenes. Mol Microbiol 6:2051-2063
6. Cummins PM, O'Connor B (1998) Pyroglutamyl peptidase: an overview of the three known enzymatic forms. Biochim Biophys Acta 1429:1-17
7. Doolittle RF, Armentrout RW (1968) Pyrrolidone peptidase. An enzyme for the selective removal of pyrrolidone carboxylic acid residues from polypeptides. Biochemistry 7:516-521
8. Edman P (1950) Method for determination of the amino acid sequence of peptides. Acta Chem Scand 4:283-293
9. Gonzales T, Robert-Baudouy J (1994) Characterization of the Pcp gene of Pseudomonas fluorescens and of its product, pyrrolidone carboxyl peptidase (Pcp). J Bacteriol 176:2569-2576
10. Griffiths EC, Kelly JA, With N, Jeffcoate SL (1980) Further studies on the inactivation of thyrotropin-releasing hormone (TRH) by enzymes in the rat hypothalamus. Acta Endocrinol 93:385-391
11. He W, Barrow CJ (1999) The Aβ 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater β-sheet forming and aggregation propensities in vitro than full length Aβ. Biochemistry 38:10871-10877
12. Henikoff S (1984) Unidirectional digestion with exonuclease-III creates targeted breakpoints for DNA sequencing. Gene (Amst) 28:351-359
13. Kawarabayasi Y, Sawada M, Horikawa H, Haikawa Y, Hino Y, Yamamoto S, Sekine M, Baba S, Kosugi H, Hosoyama A, Nagai Y, Sakai M, Ogura K, Otuka R, Nakazawa H, Takamiya M, Ohfuku Y, Funahashi T, Tanaka T, Kudoh Y, Yamazaki J, Kushida N, Oguchi A, Aoki K, Nakamura Y, Robb TF, Horikoshi K, Masuchi Y, Shizuya H, Kikuchi H (1998) Complete sequence and gene organization of the genome of a hyperthermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res 5:55-76
14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 227:680-685
15. Lesaux O, Gonzales T, Robert-Baudouy J (1996) Mutational analysis of the active site of Pseudomonas fluorescens pyrrolidone carboxyl peptidase. J Bacteriol 178:3308-3313
16. Mantle D, Lauffart B, McDermott JR, Gibson A (1990) Characterization of aminopeptidases in human kidney soluble fraction. Clin Chim Acta 187:105-114
17. Mantle D, Lauffart B, Gibson A (1991) Purification and characterisation of leucyl aminopeptidase and pyroglutamyl aminopeptidase from human skeletal muscle. Clin Chim Acta 197:35-46
18. Mozdzanoski J, Bongers J, Anumula K (1998) High yield deblocking of amino termini of recombinant immunoglobulins with pyroglutamate aminopeptidase, Anal Biochem 260:183-187
19. Mulczyk M, Szewczuk A (1970) Pyrrolidonyl peptidase in bacteria: a new colorimetric test for differentiation of Enterobacteriacaea. J Gen Microbiol 61:9-13
20. O'Connor B, O'Cuinn G (1984) Localization of a narrow-specificity thyroliberin hydrolysing pyroglutamate aminopeptidase in the synaptosomal membranes of guinea pig brain. Eur J Biochem 144:271-278
21. Odagaki Y, Hayashi A, Okada K, Hirotsu T, Kabashima K, Ito K, Yoshimoto T, Tsuru D, Sato M, Clardy J (1999) The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease. Structure (Lond) 7:399-411
22. Patti JM, Schneider A, Garza N, Boles JO (1995) Isolation and characterization of Pcp, a gene encoding a pyrrolidone carboxyl peptidase in Staphylococcus aureus. Gene (Amst) 166:95-99
23. Schauder B, Schomburg L, Kohrle J, Bauer K (1994) Cloning of a cDNA encoding an ectoenzyme that degrades thyrotropin-releasing hormone. Biochemistry 91:9534-9538
24. Singleton MR, Isupov MN, Littlechild JA (1999) X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis. Structure (Lond) 7:237-244
25. Smal MA, Dong Z, Cheung HT, Asano Y, Escoffier L, Costello M, Tattersall MH (1995) Activation and cytoxicity of 2-alpha-aminoacyl prodrugs of methotrexate. Biochem Pharmacol 49:567-574
26. Szewczuk A, Kwiatkowska J (1970) Pyrrolidonyl peptidase in animal, plant and human tissues: occurrence and some properties of the enzyme. Eur J Biochem 15:92-96
27. Tsunasawa S, Nakura S, Tanigawa T, Kato I (1998) Pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Pyrococcus furiosus: cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis. J Biochem (Tokyo) 124:778-783
28. Warburg O, Christian W (1941) Isolierung und kristallisation des Gärungs-ferments Enolase. Biochem Z 310:384-421
29. White O, Eisen JA, Heidelberg JF, Hickey EK, Peterson JD, Dodson RJ, Haft DH, Gwinn ML, Nelson WC, Richardson DL, Moffat KS, Qin H, Jiang L, Pamphile W, Crosby M, Shen M, Vamathevan JJ, Lam P, McDonald L, Utterback T, Zalewski C, Makarova KS, Aravind L, Daly MJ, Minton KW, Fleischmann RD, Ketchum KA, Nelson KE, Salzberg S, Smith HO, Venter JC, Fraser CM (1999) Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science 286:1571-1577
30. Wilk S, Wilk E (1989) Pyroglutamyl peptidase II: a thyrotropin-releasing hormone degrading enzyme: purification and specificity studies of the rabbit brain enzyme. Neurochem Int 15:81-90
31. Yoshimoto T, Shimoda T, Kitazono A, Kabashima T, Ito K, Tsuru D (1993) Pyroglutamyl peptidase gene from B. amyloliquefaciens: cloning, sequencing, expression and crystallisation of the expressed enzyme. J Biochem (Tokyo) 113:67-73