Automated multiple ligand screening by frontal affinity chromatography-mass spectrometry (FAC-MS)

Journal of Biomolecular Screening

Volumn 12, Issue 2, 2007, Pages 167-174

  Ng, William ^a   Dai, Jin Rui ^a   Slon Usakiewicz, Jacek J ^a   Redden, Peter R ^a   Pasternak, Andrew ^a   Reid, Neil ^a  

^a Transition Therapeutics   (Canada)

Author keywords

Automation; Estrogen receptor; FAC MS; Multiple ligand screening

Indexed keywords

ESTROGEN RECEPTOR BETA; LIGAND;

ANALYTIC METHOD; ARTICLE; AUTOMATION; CHROMATOGRAPHY; DRUG BINDING; ENZYME IMMOBILIZATION; FRONTAL AFFINITY CHROMATOGRAPHY; LIGAND BINDING; MASS SPECTROMETRY; PRIORITY JOURNAL; SCREENING; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AUTOMATION; CHROMATOGRAPHY, AFFINITY; ESTROGEN RECEPTOR BETA; HISTIDINE; HUMANS; LIGANDS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; SERINE;

EID: 33847628853     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057106297567     Document Type: Article

References (16)

1. Boguslavsky J: HTS assay development: is smaller really better? Drug Discov Dev 2004 ; 7: 37-40.
2. Makara GM, Atanasopoulos J: Improving success rates for lead generation using affinity binding technologies. Curr Opin Biotechnol 2005 ; 16: 666-673.
3. Annis DA, Athanasopoulos J, Curran PJ, Felsch JS, Kalghatgi K, Lee W,, et al. An affinity selection-mass spectrometry method for the identification of small molecule ligands from self-encoded combinatorial libraries: discovery of a novel antagonist of E. coli dihydrofolate reductase. Int J Mass Spectrom 2004 ; 238: 77-83.
4. Chu Y-H, Dunayevskiy YM, Kirby DP, Vouros P, Karger BL: Affinity capillary electrophoresis-mass spectrometry for screening combinatorial libraries. J Am Chem Soc 1996 ; 118: 7827-7835.
5. Muckenschnabel I, Falchetto R, Mayr LM, Filipuzzi I: Speedscreen: label-free liquid chromatography-mass spectrometry-based high-throughput screening for the discovery of orphan protein ligands. Anal Chem 2004 ; 324: 241-249.
6. Johnson BM, Nikolic D, van Breemen RB: Applications of pulsed ultracentrifugation-mass spectrometry. Mass Spectrom Rev 2002 ; 21: 76-86.
7. Loo JA: Mass spectrometry in the combinatorial chemistry revolution. Eur J Mass Spectrom 1997 ; 3: 93-104.
8. New trends in drug discovery: NMR screening. Curr Top Med Chem 2003 ; 31: 11-23.
9. Harlan JE, Egan DA, Ladror US, Snyder S, Tang MI, Buko A,, et al. Driving affinity selection by centrifugal force. Assay Drug Dev Technol 2003 ; 1: 507-519.
10. Schriemer DC, Bundle DR, Li L, Hindsgaul O: Micro-scale frontal affinity chromatography combined with mass spectrometric detection: a new method for the screening of compound libraries. Angew Chem Int Ed Engl 1997 ; 37: 3383-3387.
11. Slon-Usakiewicz JJ, Ng W, Dai JR, Pasternak A, Redden PR: Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery [review]. Drug Discov Today 2005 ; 10: 409-416.
12. Chan NWC, Lewis DF, Rosner PJ, Kelly MA, Schriemer DC: FAC-MS assay technology for multiple stages of drug discovery: applications of a chromatographic biosensor. Anal Biochem 2003 ; 319: 1-12.
13. Toledo-Sherman L, Deretey E, Slon-Usakiewicz JJ, Ng W, Dai J-R, Foster JE,, et al. Frontal affinity chromatography with MS detection of EphB2 tyrosine kinase receptor: 2. Identification of small-molecule inhibitors via coupling with virtual screening. J Med Chem 2005 ; 48: 3221-3230.
14. Rich RL, Hoth LR, Geoghegan KF, Brown TA, LeMotte PK, Simons SP,, et al. Kinetic analysis of estrogen receptor/ligand interactions. Proc Natl Acad Sci U S A 2002 ; 99: 8562-8567.
15. Annis DA, Nazef N, Chuang C-C, Scott MP, Nash HM: A general technique to rank protein-ligand binding affinities and determine allosteric versus direct binding site competition in compound mixtures. J Am Chem Soc 2004 ; 126: 15495-15503.
16. Ng ESM, Yang F, Kameyama A, Palcic MM, Hindsgaul O, Schriemer DC: High-throughput screening for enzyme inhibitors using frontal affinity chromatography and liquid chromatography and mass spectrometry. Anal Chem 2005 ; 77: 6125-6133.