The impact of severe nitrogen limitation and microaerobic conditions on extended continuous cultivations of Saccharomyces cerevisiae with cell recirculation

Enzyme and Microbial Technology

Volumn 40, Issue 4, 2007, Pages 585-593

  Brandberg, Tomas ^a   Gustafsson, Lena ^a   Franzén, Carl Johan ^a  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

Author keywords

Fuel ethanol production; Glycerol and byproduct formation; gpd1 gpd2 mutant; Growth energetics; Oxygen limitation; Yeast cell recycle

Indexed keywords

ADENOSINETRIPHOSPHATE; BIOMASS; BYPRODUCTS; CELL CULTURE; ETHANOL; ETHANOL FUELS; GLYCEROL; NITROGEN; SEDIMENTATION;

CELL RECIRCULATION; DILUTION RATES; GROWTH ENERGETICS; YEAST CELL RECYCLES;

YEAST;

ADENOSINE TRIPHOSPHATE; ALCOHOL; CELL PROTEIN; GLYCEROL; NITROGEN; OXYGEN;

AEROBIC METABOLISM; ALCOHOL PRODUCTION; ANAEROBIC METABOLISM; ARTICLE; BIOMASS PRODUCTION; BLOOD FLOW; DILUTION; ENERGY YIELD; FERMENTATION; FLUORESCENCE MICROSCOPY; FUNGAL VIABILITY; FUNGUS GROWTH; FUNGUS MUTANT; NONHUMAN; RNA ANALYSIS; SACCHAROMYCES CEREVISIAE; SEDIMENTATION RATE; STAINING;

SACCHAROMYCES CEREVISIAE;

EID: 33847320052     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2006.05.032     Document Type: Article

References (55)

1. Galbe M., and Zacchi G. A review of the production of ethanol from softwood. Appl Microbiol Biotechnol 59 (2002) 618-628
2. Albers E., Larsson C., Lidén G., Niklasson C., and Gustafsson G. Influence of the nitrogen source on Saccharomyces cerevisiae anaerobic growth and product formation. Appl Microbiol Biotechnol 62 (1996) 3187-3195
3. Holzer H., Bernhardt W., and Schneider S. Zur Glycerinbildung in Bäckerhefe. Biochemistry 336 (1963) 495-509
4. Nordström K. Yeast growth and glycerol formation. Acta Chem Scand 20 (1966) 1016-1025
5. Oura E. Reaction products of yeast fermentations. Process Biochem 249 (1977) 19-35
6. Verduyn C., Postma E., Scheffers W.A., and van Dijken J.P. Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol 136 (1990) 395-403
7. Blomberg A., and Adler L. Physiology of osmotolerance in fungi. Adv Microb Physiol 33 (1992) 145-212
8. Bailey J.E. Towards a science of metabolic engineering. Science 252 (1991) 1668-1675
9. Nissen T.L., Kielland-Brandt M.C., Nielsen J., and Villadsen J. Optimization of ethanol production in Saccharomyces cerevisiae by metabolic engineering of the ammonium assimilation. Metabol Eng 2 (2000) 69-77
10. Costenoble R., Valadi H., Gustafsson L., Niklasson C., and Franzén C.J. Microaerobic glycerol formation in Saccharomyces cerevisiae. Yeast 16 (2000) 1483-1495
11. Valadi H., Larsson C., and Gustafsson L. Improved ethanol production by glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 50 (1998) 434-439
12. Larsson C., von Stockar U., Marison I., and Gustafsson L. Growth and metabolism of Saccharomyces cerevisiae in chemostat cultures under carbon-, nitrogen, or carbon- and nitrogen-limiting conditions. J Bacteriol 175 (1993) 4809-4816
13. Larsson C., Nilsson A., Blomberg A., and Gustafsson L. Glycolytic flux is conditionally correlated with ATP concentration in Saccaromyces cerevisiae: a chemostat study under carbon- or nitrogen-limiting conditions. J Bacteriol 179 (1997) 7243-7250
14. Lidén G., Persson A., Gustafsson L., and Niklasson C. Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation. Appl Microbiol Biotechnol 43 (1995) 1034-1038
15. Lillie S.H., and Pringle J.R. Reserve carbohydrate metabolism in Saccharomyces cerevisiae: responses to nutrient limitation. J Bacteriol 143 (1980) 1384-1394
16. Schulze U., Lidén G., Nielsen J., and Villadsen J. Physiological effects of nitrogen starvation in an anaerobic batch culture of Saccharomyces cerevisiae. Microbiology 142 (1996) 2299-2310
17. Jørgensen H., Olsson L., Rønnow B., and Palmqvist E.A. Fed-batch cultivation of baker's yeast followed by nitrogen or carbon starvation: effects on fermentative capacity and content of trehalose and glycogen. Appl Microbiol Biotechnol 59 (2002) 310-317
18. Busturia A., and Lagunas R. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae. J Gen Microbiol 132 (1986) 379-385
19. Johnston G.C., and Pringle J.R. Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res 105 (1977) 79-98
20. López S., and Gancedo J.M. Effect of metabolic conditions on protein turnover in yeast. Biochem J 178 (1979) 769-776
21. Nilsson A., Norbeck J., Ölz R., Blomberg A., and Gustafsson L. Fermentative capacity after cold storage of bakers' yeast is dependent on the initial physiological state but not correlated to the levels of glycolytic enzymes. Int J Food Microbiol 71 (2001) 111-124
22. Nilsson A., Påhlman I.-L., Jovall P.-A., Blomberg A., Larsson C., and Gustafsson L. The catabolic capacity of Saccharomyces cerevisiae is preserved to a higher extent during carbon compared to nitrogen starvation. Yeast 18 (2001) 1-12
23. Thomsson E., Larsson C., Albers E., Nilsson A., Franzén C.J., and Gustafsson L. Carbon starvation can induce energy deprivation and loss of fermentative capacity in Saccharomyces cerevisiae. Appl Env Microbiol 69 (2003) 3251-3257
24. Shkidchenko A.N. Limitation of growth of the yeast Saccharomyces cerevisiae in a chemostat system by the carbon and nitrogen sources. Microbiology 53 (1984) 44-48
25. Franzén C.J., Lidén G., and Niklasson C. An experimental guide to the relevant aeration rates in microaerobic bioprocesses. Advances in bioprocess engineering (1994), Kluwer Academic Publishers, The Netherlands p. 255-65
26. Franzén C.J. Metabolic flux analysis of RQ-controlled microaerobic ethanol production by Saccharomyces cerevisiae. Yeast 20 (2003) 117-132
27. Hoppe G.K., and Hansford G.S. The effect of micro-aerobic conditions on continuous ethanol production by Saccharomyces cerevisiae. Biotechnol Lett 6 (1984) 681-686
28. Ryu D.D.Y., Kim Y.J., and Kim J.H. Effect of air supplement on the performance of continuous ethanol fermentation system. Biotechnol Bioeng 26 (1984) 12-16
29. Cysewski G.R., and Wilke C.R. Rapid ethanol fermentation using vacuum and cell recycle. Biotechnol Bioeng 19 (1977) 805-819
30. Cysewski G.R., and Wilke C.R. Process design and economic studies of alternative fermentation methods for the production of ethanol. Biotechnol Bioeng 20 (1978) 1421-1444
31. Wada M., Jato J., and Chibata I. Continuous production of ethanol in high concentration using immobilized growing yeast cells. Eur J Appl Microbiol Biotechnol 11 (1981) 67-71
32. Taniguchi M., Wakamiya K., Tsuchiya M., Matsuno R., and Kamikubo T. Continuous ethanol production by cell-holding culture of yeast. Eur J Appl Microbiol Biotechnol 18 (1983) 201-206
33. van Hoek P., de Hulster E., van Dijken J.P., and Pronk J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker's yeast. Biotechnol Bioeng 68 (1999) 517-523
34. Brandberg T., Franzén C.J., and Gustafsson L. Strain comparison of Saccharomyces cerevisiae for anaerobic fermentation of wood hydrolysate. J Biosci Bioeng 98 (2004) 122-125
35. Brandberg T., Sanandaji N., Franzén C.J., and Gustafsson L. Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate with Saccharomyces cerevisiae ATCC 96581 using cell recirculation. Biotechnol Prog 21 (2005) 1093-1101
36. Lindén T., Peetre J., and Hahn-Hägerdahl B. Isolation and characterisation of acetic acid tolerant galactose-fermenting strains of Saccharomyces cerevisiae from a spent sulfite liquor plant. Appl Env Microbiol 58 (1992) 1661-1669
37. Martín C., and Jönsson L.J. Comparison of the resistance of industrial and laboratory strains of Saccharomyces and Zygosaccharomyces to lignocellulose-derived fermentation inhibitors. Enzyme Microb Technol 32 (2003) 386-395
38. +-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation. EMBO J 16 (1997) 128-132
39. Franzén C.J., Albers E., and Niklasson C. Use of the inlet gas composition to control the respiratory quotient in microaerobic bioprocesses. Chem Eng Sci 51 (1996) 3391-3402
40. Schulze U., Elleskov Larsen M., and Villadsen J. Determination of intracellular trehalose and glycogen in Saccharomyces cerevisiae. Anal Biochem 228 (1995) 143-149
41. Nielsen J., Villadsen J., and Lidén G. Bioreaction engineering principles (2002), Kluwer Academic/Plenum Publishers, NY p. 55
42. Stephanopoulos G.N., Aristidou A.A., and Nielsen J. Metabolic engineering-principles and methodologies (1998), Academic Press, CA p. 57-76
43. Aguilaniu H., Gustafsson L., Rigoulet M., and Nyström T. Assymetric inheritance of oxidatively damaged proteins during cytokinesis in Saccharomyces cerevisiae. Science 299 (2003) 1751-1753
44. Albers E., Lidén G., Larsson C., and Gustafsson L. Anaerobic redox balance and nitrogen metabolism in Saccharomyces cerevisae. In: Pandalai S.G. (Ed). Recent research developments in microbiology, vol. 2 (1998), Research Sign Post, Trivandrum, India 253-279
45. van Dijken J.P., and Scheffers W.A. Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol Rev 32 (1986) 199-224
46. Larsson C., Påhlman I.-L., Ansell R., Rigoulet M., Adler L., and Gustafsson L. The importance of the glycerol 3-phosphate shuttle during aerobic growth of Saccharomyces cerevisiae. Yeast 14 (1998) 347-357
47. Luttik M.A.H., Overkamp K.M., Kötter P., de Vries S., van Dijken J.P., and Pronk J.T. The Saccharomyces NDE1 and NDE2 genes encode separate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. J Biol Chem 273 (1998) 24529-24534
48. Weusthuis R.A., Visser W., Pronk J.T., Scheffers W.A., and van Dijken J.P. Effects of oxygen limitation on sugar metabolism in yeasts: a continuous culture study of the Kluyver effect. Microbiology 140 (1994) 703-715
49. Hohmann S. Osmotic stress signaling and osmoadaptation in yeasts. Microbiol Mol Biol Rev 66 (2002) 300-372
50. Björkqvist S., Ansell R., Adler L., and Lidén G. Physiological response to anaerobicity of glycerol-3-phosphate dehydrogenase mutants of Saccharomyces cerevisiae. Appl Env Microbiol 63 (1997) 128-132
51. Chung I.S., and Lee Y.Y. Ethanol fermentation of crude acid hydrolysate of cellulose using high-level yeast inocula. Biotechnol Bioeng 27 (1985) 308-315
52. Lee W.G., Lee J.S., Park B.G., Kim M.S., Park S.C., and Chang H.N. Continuous ethanol production from wood hydrolysate by chemostat and total cell retention culture. Korean J Chem Eng 13 (1996) 453-456
53. Lee W.G., Lee J.S., Shin C.S., Park S.C., Chang H.N., and Chang Y.K. Ethanol production using concentrated oak wood hydrolysates and methods to detoxify. Appl Biochem Biotechnol 77-79 (1999) 547-559
54. Lee W.G., Park B.G., Chang Y.K., Chang H.N., Lee J.S., and Park S.C. Continuous ethanol production from concentrated wood hydrolysates in an internal membrane-filtration bioreactor. Biotechnol Prog 16 (2000) 302-304
55. Palmqvist E., Galbe M., and Hahn-Hägerdal B. Evaluation of cell recycling in continuous fermentation of enzymatic hydrolysates of spruce with Saccharomyces cerevisiae and on-line monitoring of glucose and ethanol. Appl Microbiol Biotechnol 50 (1998) 545-551