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Direct observation of kinesin stepping by optical trapping interferometry
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Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization
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A. Yildiz, J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin, "Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization," Science 300, 2061-2065 (2003).
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3
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Kinesin and dynein move a peroxisome in vivo: A tug-of-war or coordinated movement?
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C. Kural, H. Kim, S. Syed, G. Goshima, V. I. Gelfand, and P. R. Selvin, "Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement?" Science 308, 1469-1472 (2005).
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Nanoscale resolution in the focal plane of an optical microscope
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Single myosin molecule mechanics: Piconewton forces and nanometre steps
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Myosin-V is a processive actin-based motor
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7
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0029416931
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Transcription against an applied force
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H. Yin, M. D. Wang, K. Svoboda, R. Landick, S. M. Block, and J. Gelles, "Transcription against an applied force," Science 270, 1653-1657 (1995).
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Yin, H.1
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8
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9144271132
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Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detection
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L. Nugent-Glandorf and T. T. Perkins, "Measuring 0.1-nm motion in 1 ms in an optical microscope with differential back-focal-plane detection," Opt. Lett. 29, 2611-2613 (2004).
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10
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28544432440
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Direct observation of base-pair stepping by RNA polymerase
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E. A. Abbondanzieri, W. J. Greenleaf, J. W. Shaevitz, R. Landick, and S. M. Block, "Direct observation of base-pair stepping by RNA polymerase," Nature 438, 460-465 (2005).
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Nature
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Abbondanzieri, E.A.1
Greenleaf, W.J.2
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Block, S.M.5
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11
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33744981369
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Sequence-resolved detection of pausing by single RNA polymerase molecules
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K. M. Herbert, A. La Porta, B. J. Wong, R. A. Mooney, K. C. Neuman, R. Landick, and S. M. Block, "Sequence-resolved detection of pausing by single RNA polymerase molecules," Cell 125, 1083-1094 (2006).
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Cell
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Herbert, K.M.1
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Wong, B.J.3
Mooney, R.A.4
Neuman, K.C.5
Landick, R.6
Block, S.M.7
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12
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1542375262
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Forward and reverse motion of single RecBCD molecules on DNA
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T. T. Perkins, H. W. Li, R. V. Dalal, J. Gelles, and S. M. Block, "Forward and reverse motion of single RecBCD molecules on DNA," Biophys. J. 86, 1640-1648 (2004).
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Perkins, T.T.1
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13
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0032582494
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Force and velocity measured for single molecules of RNA polymerase
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M. D. Wang, M. J. Schnitzer, H. Yin, R. Landick, J. Gelles, and S. M. Block, "Force and velocity measured for single molecules of RNA polymerase," Science 282, 902-907 (1998).
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Wang, M.D.1
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Block, S.M.6
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14
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0031047888
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Stretching DNA with optical tweezers
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M. D. Wang, H. Yin, R. Landick, J. Gelles, and S. M. Block, "Stretching DNA with optical tweezers," Biophys. J. 72, 1335-1346 (1997).
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Wang, M.D.1
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Mapping the actin filament with myosin
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Position control and optical manipulation for nanotechnology applications
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M. Capitanio, R. Cicchi, and F. S. Pavone, "Position control and optical manipulation for nanotechnology applications," Eur. Phys. J. B 46, 1-8 (2005).
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0030358936
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Construction of multiple-beam optical traps with nanometer-resolution position sensing
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Interference model for back-focal-plane displacement detection in optical tweezers
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Optical measurement of picometer displacements of transparent microscopic objects
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84894004216
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We note that our differential BFP detection is immune to common mode fluctuations such as air currents and lens motion. However, a large fraction (40, of the optical path is not common mode, and the common mode optical elements (excluding the objective) are rigidly attached to the microscope frame or the optical table by custom-made, large-diameter (>38 mm) aluminum posts. Vibrational testing suggests that the current limits in the mechanical stability of our system are the fiber launches and the QPDs, which are independent for each laser; therefore, the second, 850 nm laser represents an independent measurement
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We note that our differential BFP detection is immune to common mode fluctuations such as air currents and lens motion. However, a large fraction (40%) of the optical path is not common mode, and the common mode optical elements (excluding the objective) are rigidly attached to the microscope frame or the optical table by custom-made, large-diameter (>38 mm) aluminum posts. Vibrational testing suggests that the current limits in the mechanical stability of our system are the fiber launches and the QPDs, which are independent for each laser; therefore, the second, 850 nm laser represents an independent measurement.
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22
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84894005789
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The laser diode was driven by custom electronics that stabilized the temperature to ±15 mK/°C ambient temperature variation. The current stability of the driver was 25 ppm/°C. The manufacturer's specification of the laser diode's spectral linewidth is ∼0.5 nm FWHM.
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The laser diode was driven by custom electronics that stabilized the temperature to ±15 mK/°C ambient temperature variation. The current stability of the driver was 25 ppm/°C. The manufacturer's specification of the laser diode's spectral linewidth is ∼0.5 nm FWHM.
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23
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85043729189
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-5). Several identical lasers performed only 10% better than specification after the PBS. In general, intensity stabilization will be required to achieve 0.1 nm vertical resolution.
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-5). Several identical lasers performed only 10% better than specification after the PBS. In general, intensity stabilization will be required to achieve 0.1 nm vertical resolution.
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24
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20444507097
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Measurement of the effective focal shift in an optical trap
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K. C. Neuman, E. A. Abbondanzieri, and S. M. Block, "Measurement of the effective focal shift in an optical trap," Opt. Lett. 30, 1318-1320 (2005).
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Neuman, K.C.1
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25
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0002053947
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Three-dimensional siloxane resist for the formation of nanopatterns with minimum linewidth fluctuations
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H. Namatsu, Y. Takahashi, K. Yamazaki, T. Yamaguchi, M. Nagase, and K. Kurihara, "Three-dimensional siloxane resist for the formation of nanopatterns with minimum linewidth fluctuations," J. Vac. Sci. Technol. B 16, 69-76 (1998).
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26
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0032707613
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Characterization of photodamage to escherichia coli in optical traps
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K. C. Neuman, E. H. Chadd, G. F. Liou, K. Bergman, and S. M. Block, "Characterization of photodamage to escherichia coli in optical traps," Biophys. J. 77, 2856-2863 (1999).
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Neuman, K.C.1
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Block, S.M.5
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27
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84893992156
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Increasing the laser power created a drift in the positive z direction. Decreasing the laser power caused a negative z drift. This drift corresponded to a movement of the laser focus (set by the objective) relative to the fiducial mark (set by the sample). Furthermore, drift rates increased linearly with the change in laser power. Finally, after ∼15 min at a particular laser power the drift would settle, indicating a new equilibrium had been reached. Since all other optical components have >97% transmission at 1064 nm, these data are best explained by the thermal expansion (or contraction) of the objective as the main source of this drift since its transmission at 1064 nm is 59%.
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Increasing the laser power created a drift in the positive z direction. Decreasing the laser power caused a negative z drift. This drift corresponded to a movement of the laser focus (set by the objective) relative to the fiducial mark (set by the sample). Furthermore, drift rates increased linearly with the change in laser power. Finally, after ∼15 min at a particular laser power the drift would settle, indicating a new equilibrium had been reached. Since all other optical components have >97% transmission at 1064 nm, these data are best explained by the thermal expansion (or contraction) of the objective as the main source of this drift since its transmission at 1064 nm is 59%.
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28
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0028362896
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Force and velocity measured for single kinesin molecules
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K. Svoboda and S. M. Block, "Force and velocity measured for single kinesin molecules," Cell 77, 773-784 (1994).
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Svoboda, K.1
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0942279641
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Kinesin walks hand-over-hand
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Yildiz, A.1
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30
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0028956979
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Measurement of lactose repressor-mediated loop formation and breakdown in single DNA molecules
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M. E. J. Friese, H. Rubinsztein-Dunlop, N. R. Heckenberg, and E. W. Dearden, "Determination of the force constant of a single-beam gradient trap by measurement of backscattered light," Appl. Opt. 35, 7112-7116 (1996).
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Friese, M.E.J.1
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