Access of the substrate to the active site of yeast oxidosqualene cyclase: An inhibition and site-directed mutagenesis approach

ChemBioChem

Volumn 6, Issue 12, 2005, Pages 2221-2228

  Oliaro Bosso, Simonetta ^a   Schulz Gasch, Tanja ^b   Balliano, Gianni ^a   Viola, Franca ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Cyclases; Enzyme models; Inhibitors; Mutagenesis; Steroids

Indexed keywords

ALANINE; AMINO ACID; ASPARTIC ACID; CYSTEINE; GLUTAMIC ACID; GLYCINE; LAURYL ALCOHOL; MALEIMIDE DERIVATIVE; MUTANT PROTEIN; OXIDOSQUALENE CYCLASE; PHENYLALANINE; PROLINE; SQUALENE DERIVATIVE; THIOL; UNCLASSIFIED DRUG; VALINE; ENZYME INHIBITOR; LANOSTEROL SYNTHASE; MUTASE; SACCHAROMYCES CEREVISIAE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING SITE; CATALYSIS; CONTROLLED STUDY; GENETIC CONSERVATION; HYDROGEN BOND; LIGATION; MOLECULAR MODEL; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVITY; THERMOSTABILITY; YEAST; CHEMISTRY; DRUG ANTAGONISM; ENZYME SPECIFICITY; ENZYME STABILITY; GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS;

SACCHAROMYCES CEREVISIAE;

BINDING SITES; CONSERVED SEQUENCE; ENZYME INHIBITORS; ENZYME STABILITY; INTRAMOLECULAR TRANSFERASES; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; SACCHAROMYCES CEREVISIAE PROTEINS; SUBSTRATE SPECIFICITY;

EID: 33846613345     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200500107     Document Type: Article

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