Nef protein of human immunodeficiency virus type 1 binds its own myristoylated N-terminus

Biological Chemistry

Volumn 388, Issue 2, 2007, Pages 181-183

  Hoffmann, Silke ^a,b   Jonas, Esther ^a,b   König, Simone ^c   Preusser Kunze, Andrea ^d,e   Willbold, Dieter ^a,b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b FORSCHUNGSZENTRUM JÜLICH   (Germany)

^c UNIVERSITY OF MÜNSTER   (Germany)

^d FRITZ LIPMANN INSTITUTE   (Germany)

^e UNIVERSITY OF GÖTTINGEN   (Germany)

Author keywords

Fluorescence titration; Peptide binding; Protein conformation

Indexed keywords

MYRISTIC ACID; NEF PROTEIN; SYNTHETIC PEPTIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; FLUORESCENCE; HUMAN IMMUNODEFICIENCY VIRUS 1; MYRISTYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; TITRIMETRY;

CALORIMETRY; GENE PRODUCTS, NEF; HUMANS; MYRISTIC ACID; NEF GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; PEPTIDES; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 33846569017     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2007.020     Document Type: Article

References (15)

1. Arold, S., Hoh, F., Domergue, S., Birck, C., Delsuc, M.A., Jullien, M., and Dumas, C. (2000). Characterization and molecular basis of the oligomeric structure of HIV-1 nef protein. Protein Sci. 9, 1137-1148.
2. Arold, S.T. and Baur, A.S. (2001). Dynamic Nef and Nef dynamics: how structure could explain the complex activities of this small HIV protein. Trends Biochem. Sci. 26, 356-363.
3. Bell, I., Schaefer, T.M., Trible, R.P., Amedee, A., and Reinhart, T.A. (2001). Down-modulation of the costimulatory molecule, CD28, is a conserved activity of multiple SIV Nefs and is dependent on histidine 196 of Nef. Virology 283, 148-158.
4. Bentham, M., Mazaleyrat, S., and Harris, M. (2006). Role of myristoylation and N-terminal basic residues in membrane association of the human immunodeficiency virus type 1 Nef protein. J. Gen. Virol. 87, 563-571.
5. Dennis, C.A., Baron, A., Grossmann, J.G., Mazaleyrat, S., Harris, M., and Jaeger, J. (2005). Co-translational myristoylation alters the quaternary structure of HIV-1 Nef in solution. Proteins 60, 658-669.
6. Geyer, M., Fackler, O.T., and Peterlin, B.M. (2001). Structure-function relationships in HIV-1 Nef. EMBO Rep. 2, 580-585.
7. Haller, C., Rauch, S., Michel, N., Hannemann, S., Lehmann, M.J., Keppler, O.T., and Fackler, O.T. (2006). The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity. J. Biol. Chem. 281, 19618-19630.
8. Hrecka, K., Swigut, T., Schindler, M., Kirchhoff, F., and Skowronski, J. (2005). Nef proteins from diverse groups of primate lentiviruses downmodulate CXCR4 to inhibit migration to the chemokine stromal derived factor 1. J. Virol. 79, 10650-10659.
9. 13C. J. Biomol. NMR 12, 109-121.
10. Mangasarian, A., Piguet, V., Wang, J.K., Chen, Y.L., and Trono, D. (1999). Nef-induced CD4 and major histocompatibility complex class I (MHC-I) down-regulation are governed by distinct determinants: N-terminal α-helix and proline repeat of Nef selectively regulate MHC-I trafficking. J. Virol. 73, 1964-1973.
11. Preusser, A., Briese, L., Baur, A.S., and Willbold, D. (2001). Direct in vitro binding of full-length human immunodeficiency virus type 1 Nef protein to CD4 cytoplasmic domain. J. Virol. 75, 3960-3964.
12. Renkema, G.H. and Saksela, K. (2000). Interactions of HIV-1 NEF with cellular signal transducing proteins. Front. Biosci. 5, D268-D283.
13. Schindler, M., Munch, J., Kutsch, O., Li, H., Santiago, M.L., Bibollet-Ruche, F., Muller-Trutwin, M.C., Novembre, F.J., Peeters, M., Courgnaud, V., et al. (2006). Nef-mediated suppression of T cell activation was lost in a lentiviral lineage that gave rise to HIV-1. Cell 125, 1055-1067.
14. Stove, V., Van de Walle, I., Naessens, E., Coene, E., Stove, C., Plum, J., and Verhasselt, B. (2005). Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8αβ. J. Virol. 79, 11422-11433.
15. Swigut, T., Shohdy, N., and Skowronski, J. (2001). Mechanism for down-regulation of CD28 by Nef. EMBO J. 20, 1593-1604.