Improving the thermal stability of lactate oxidase by directed evolution

Cellular and Molecular Life Sciences

Volumn 64, Issue 1, 2007, Pages 77-81

  Minagawa, H ^a   Yoshida, Y ^a   Kenmochi, N ^b   Furuichi, M ^a   Shimada, J ^a   Kaneko, H ^b  

^a NEC CORPORATION   (Japan)

^b NIHON UNIVERSITY   (Japan)

Author keywords

Directed evolution; DNA shuffling; Lactate oxidase; Thermostability

Indexed keywords

DNA; LACTATE 2 MONOOXYGENASE;

AEROCOCCUS VIRIDANS; AMINO ACID SEQUENCE; ARTICLE; BIOSENSOR; CELL FREE SYSTEM; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ENZYME METABOLISM; ENZYME STABILITY; GENE LIBRARY; GENE MUTATION; GENETIC SCREENING; INDUCED MUTATION; LACTATE BLOOD LEVEL; MICROARRAY ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; THERMOSTABILITY; WILD TYPE;

AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MUTATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; STREPTOCOCCACEAE; TEMPERATURE;

AEROCOCCUS VIRIDANS;

EID: 33846528000     PISSN: 1420682X     EISSN: 15691632     Source Type: Journal    
DOI: 10.1007/s00018-006-6409-8     Document Type: Article

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