Effect of mutations at Glu160 and Val198 on the thermostability of lactate oxidase

European Journal of Biochemistry

Volumn 270, Issue 17, 2003, Pages 3628-3633

  Minagawa, Hirotaka ^a   Shimada, Jiro ^a   Kaneko, Hiroki ^b  

^a NEC CORPORATION   (Japan)

^b NIHON UNIVERSITY   (Japan)

Author keywords

Lactate oxidase; Random mutagenesis; Site directed mutagenesis; Thermostability

Indexed keywords

LACTATE 2 MONOOXYGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; ELECTRICITY; ENZYME INACTIVATION; ENZYME STABILITY; GENE CONSTRUCT; HALF LIFE TIME; MOLECULAR MODEL; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO ACIDS; ELECTROSTATICS; ENZYME STABILITY; ESCHERICHIA COLI; GLUTAMIC ACID; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SPINACIA OLERACEA; TEMPERATURE; VALINE;

EID: 0041823542     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03751.x     Document Type: Article

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