Immobilized preparation of cold-adapted and halotolerant Antarctic β-galactosidase as a highly stable catalyst in lactose hydrolysis

FEMS Microbiology Ecology

Volumn 59, Issue 2, 2007, Pages 535-542

  Makowski, Krzysztof ^a   Białkowska, Aneta ^a   Szczȩsna Antczak, Mirosława ^a   Kalinowska, Halina ^a   Kur, Józef ^b   Cieśliński, Hubert ^b   Turkiewicz, Marianna ^a  

^a LODZ UNIVERSITY OF TECHNOLOGY   (Poland)

^b GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

galactosidase; Antarctic; Chitosan beads; Immobilized enzyme; Lactose hydrolysis in cold

Indexed keywords

BACTERIAL ENZYME; BETA GALACTOSIDASE; CALCIUM ION; CHITOSAN; GALACTOSE; GLUCOSE; GLUTARALDEHYDE; LACTOSE; SODIUM CHLORIDE;

CATALYSIS; COLD TOLERANCE; ENZYME ACTIVITY; HYDROLYSIS; IMMOBILIZATION; SALINITY TOLERANCE;

ANTARCTICA; BACTERIAL GENETICS; CATALYST; COLD ACCLIMATIZATION; CONCENTRATION RESPONSE; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; HYDROLYSIS; MARINE BACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PSEUDOALTEROMONAS; SALT TOLERANCE;

ANIMALS; ANTARCTIC REGIONS; BETA-GALACTOSIDASE; BIOTECHNOLOGY; CATALYSIS; CHITOSAN; COLD; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; HYDROLYSIS; LACTOSE; MICROSPHERES; MILK; PSEUDOALTEROMONAS; SODIUM CHLORIDE;

ANTARCTICA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOALTEROMONAS SP. 22B;

EID: 33846248256     PISSN: 01686496     EISSN: 15746941     Source Type: Journal    
DOI: 10.1111/j.1574-6941.2006.00208.x     Document Type: Conference Paper

References (24)

1. Albayrak N & Yang ST (2002) Immobilization of Aspergillus oryzae β-galactosidase on tosylated cotton cloth. Enz Microb Technol 31: 371-383.
2. Bakken AP, Hill CG & Amundson CH (1992) Hydrolysis of lactose in skim milk by immobilized β-galactosidase (Bacillus circulans). Biotechnol Bioeng 39: 408-417.
3. Beccera M, Baroli B, Fadda A, Blanco Mendez J & Gonzales MI (2001) Lactose bioconversion by calcium-alginate immobilization of Kluyveromyces lactis cells. Enz Microb Technol 29: 506-512.
4. Bodalo A, Gomez E, Gomez JL, Bastida J, Maximo MF & Diaz F (1998) A comparison of different methods of β-galactosidase immobilization. Proc Biochem 26: 349-353.
5. Cetinus SA & Oztop HN (2003) Immobilization of catalase into chemically crosslinked chitosan beads. Enz Microb Technol 32: 889-894.
6. Cieśliński H, Kur J, Białkowska A, Baran I, Makowski K & Turkiewicz M (2005) Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from Antarctic bacterium Pseudoalteromonas sp. 22b. Protein Expr Purif 39: 27-39.
7. Fernandes S, Geueke B, Delgado O, Coleman J & Hatti-Kaul R (2002) β-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis. Appl Microbiol Biotechnol 58: 313-321.
8. Georlette D, Blaise V, Collins T, D'Amico S, Gratia E, Hoyoux A, Marx J-C, Sonan G, Feller G & Gerday C (2004) Some like it cold: biocatalysis at low temperatures. FEMS Microbiol Rev 28: 25-42.
9. Hoyoux IJ, Dubois P, Genicot S, Dubail F, François JM, Baise E, Feller G & Gerday C (2001) Cold-adapted β-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis. Appl Environ Microbiol 67: 1529-1535.
10. Ladero M, Santos A, Garcia JL & Garcia-Ochoa F (2000) Activity over lactose and ONPG of a genetically engineered β-galactosidase from Escherichia coli in solution and immobilized: kinetic modelling. Enz Microb Technol 29: 181-193.
11. Lowry OH, Rosbrough NJ, Farr AL & Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275.
12. Manjon A, Llorca FI, Bonete MJ, Bastida J & Iborra JL (1985) Properties of β-galactosidase covalently immobilized to glycophase-coated porous glass. Proc Biochem 20: 17-22.
13. Mateo C, Monti R, Pessela BC, Fuentes M, Torres R, Guisan JM & Fernandez-Lafuente R (2004) Immobilization of lactase from Kluyveromyces lactis greatly reduces inhibition promoted by glucose. Full hydrolysis of lactose in milk. Biotechnol Prog 20: 1259-1262.
14. Pessela BC, Mateo C, Carrascosa AV, Vian A, Garcia JL, Rivas G, Alfonso C, Guisan JM & Fernandez-Lafuente R (2003) One-step purification, covalent immobilization, and additional stabilization of a thermophilic ply-His-tagged beta-galactosidase from Thermus sp. Strain T2 by using novel heterofunctional chelate-epoxy Sepabeads. Biomacromolecules 4: 107-113.
15. Portaccio M, Stellato S, Rossi S, Bencivenga U, Mohy Eldin MS, Gaeta FS & Mita DG (1998) Galactose competitive inhibition of β-galactosidase (Aspergillus oryzae) immobilized on chitosan and nylon supports. Enz Microb Technol 23: 101-106.
16. Roy I & Gupta MN (2003) Lactose hydrolysis by lactozym™ immobilized on cellulose beads in batch and fluized bed modes. Proc Biochem 39: 325-332.
17. Sheridan P, Panasik N, Coombs J & Brenchley J (2000) Approaches to deciphering the structural basis of low temperature enzyme activity. Biochem Biophys Acta 1543: 413-429.
18. Sheu DC, Li SY, Duan KJ & Chen CW (1998) Production of galactooligosaccharides by β-galactosidase immobilized on glutaraldehyde-treated chitosan beads. Biotechnol Techn 12: 273-276.
19. Szczodrak J (2000) Hydrolysis of lactose in whey permeate by immobilized β-galactosidase from Kluyveromyces fragilis. J Mol Cat B 10: 631-637.
20. Tanriseven A & Dogan S (2002) A novel method for the immobilization of β-galactosidase. Proc Biochem 38: 27-30.
21. Tkaczuk KL, Bujnicki JM, Białkowska A, Bielecki S, Turkiewicz M, Cieśliński H & Kur J (2005) Molecular modelling of a psychrophilic beta-galactosidase. Biocatal Biotransform 23: 201-209.
22. Turkiewicz M, Białkowska A, Kalinowska H & Bielecki S (2003a) Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold-adapted β-galactosidase. Biomol Eng 20: 317-324.
23. Turkiewicz M, Makowski K, Białkowska A & Szczȩsna- Antczak M (2003b) The immobilized Antarctic β-galactosidase and its application for lactose hydrolysis. 11th European Congress on Biotechnology, 24-29 August 2003. Basel, Switzelrland. Abstracts, p. 710.
24. Zhou QZK & Chen XD (2001) Immobilization of β-galactosidase on graphite surface by glutaraldehyde. J Food Eng 48: 69-74.