Activity over lactose and ONPG of a genetically engineered β-galactosidase from Escherichia coli in solution and immobilized: Kinetic modelling

Enzyme and Microbial Technology

Volumn 29, Issue 2-3, 2001, Pages 181-193

  Ladero, M ^a   Santos, A ^a   Garcia J L ^b   Garcia Ochoa F ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

galactosidase; Activity; Escherichia coli; Hydrolysis; Immobilization; Kinetic model; Lactose; ONPG

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYME IMMOBILIZATION; ENZYME INHIBITION; ENZYMES; GENETIC ENGINEERING; HYDROLYSIS; PH; STABILITY;

KINETIC MODELLING;

ESCHERICHIA COLI;

ALUMINUM OXIDE; BETA GALACTOSIDASE; GALACTOSE; GALACTOSIDE; GLUCOSE; LACTOSE; O NITROPHENOL BETA DEXTRO GALACTOSIDE; SILICON DIOXIDE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CATALYST; COMPETITIVE INHIBITION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC ENGINEERING; HYDROLYSIS; KINETICS; MATHEMATICAL MODEL; MICHAELIS CONSTANT; NONHUMAN; PH; PURIFICATION; TEMPERATURE;

ESCHERICHIA COLI;

EID: 0035822773     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(01)00366-0     Document Type: Article

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