A novel MDR1 G1199T variant alters drug resistance and efflux transport activity of P-glycoprotein in recombinant Hek cells

Journal of Pharmaceutical Sciences

Volumn 95, Issue 12, 2006, Pages 2767-2777

  Crouthamel, Matthew H ^a   Wu, Daniel ^a   Yang, Ziping ^a   Ho, Rodney J Y ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Drug resistance; Efflux pumps; Genetic variants; Multidrug resistance; Multidrug resistance transporter; P glycoprotein

Indexed keywords

DOXORUBICIN; GLYCOPROTEIN P; MULTIDRUG RESISTANCE PROTEIN 1; PACLITAXEL; TOPOTECAN; VINBLASTINE; VINCRISTINE;

ARTICLE; CANCER PATIENT; CELL STRAIN HEK293; CONTROLLED STUDY; DNA POLYMORPHISM; DRUG RESISTANCE; GENE FREQUENCY; GENETIC VARIABILITY; HUMAN; HUMAN CELL; LEUKEMIA; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN VARIANT;

EID: 33846200440     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.20743     Document Type: Article

References (17)

1. Lin JH, Yamazaki M. 2003. Clinical relevance of P-glycoprotein in drug therapy. Drug Metab Rev 35:417-454.
2. Ambudkar SV, Kimchi-Sarfaty C, Sauna ZE, Gottesman MM. 2003. P-glycoprotein: From genomics to mechanism. Oncogene 22:7468-7485.
3. Ambudkar SV, Dey S, Hrycyna CA, Ramachandra M, Pastan I, Gottesman MM. 1999. Biochemical, cellular, and pharmacological aspects of the multidrug transporter. Annu Rev Pharmacol Toxicol 39:361-398.
4. Schinkel AH, Mayer U, Wagenaar E, Mol CA, van Deemter L, Smit JJ, van der Valk MA, Voordouw AC, Spits H, van Tellingen O, Zijlmans JM, Fibbe WE, Borst P. 1997. Normal viability and altered pharmacokinetics in mice lacking mdr1-type (drug-transporting) P-glycoproteins. Proc Natl Acad Sci USA 94:4028-4033.
5. Woodahl EL, Yang Z, Bui T, Shen DD, Ho RJ. 2004. Multidrug resistance gene G1199A polymorphism alters efflux transport activity of P-glycoprotein. J Pharmacol Exp Ther 310:1199-1207.
6. Cascorbi I, Gerloff T, Johne A, Meisel C, Hoffmeyer S, Schwab M, Schaeffeler E, Eichelbaum M, Brinkmann U, Roots I. 2001. Frequency of single nucleotide polymorphisms in the T-glycoprotein drug transporter MDR1 gene in white subjects. Clin Pharmacol Ther 69:169-174.
7. Hoffmeyer S, Burk O, von Richter O, Arnold HP, Brockmoller J, Johne A, Cascorbi I, Gerloff T, Roots I, Eichelbaum M, Brinkmann U. 2000. Functional polymorphisms of the human multidrug-resistance gene: Multiple sequence variations and correlation of one allele with P-glycoprotein expression and activity in vivo. Proc Natl Acad Sci USA 97:3473-3478.
8. Kim RB, Leake BF, Choo EF, Dresser GK, Kubba SV, Schwarz UI, Taylor A, Xie HG, McKinsey J, Zhou S, Lan LB, Schuetz JD, Schuetz EG, Wilkinson GR. 2001. Identification of functionally variant MDR1 alleles among European Americans and African Americans. Clin Pharmacol Ther 70:189-199.
9. Woodahl EL, Ho RJ. 2004. The role of MDR1 genetic polymorphisms in interindividual variability in P-glycoprotein expression and function. Curr Drug Metab 5:11-19.
10. Thomas P, Smart TG. 2005. HEK293 cell line: A vehicle for the expression of recombinant proteins. J Pharmacol Toxicol Methods 51:187-200.
11. Yang Z, Woodahl EL, Wang XY, Bui T, Shen DD, Ho RJ. 2002. Semi-quantitative RT-PCR method to estimate full-length mRNA levels of the multidrug resistance gene. Biotechniques 33:196, 198, 200 passim.
12. Vethanayagam RR, Wang H, Gupta A, Zhang Y, Lewis F, Unadkat JD, Mao Q. 2005. Functional analysis of the human variants of breast cancer resistance protein: I206L, N590Y, and D620N. Drug Metab Dispos 33:697-705.
13. Kimchi-Sarfaty C, Gribar JJ, Gottesman MM. 2002. Functional characterization of coding polymorphisms in the human MDR1 gene using a vaccinia virus expression system. Mol Pharmacol 62:1-6.
14. Bodor M, Kelly EJ, Ho RJ. 2005. Characterization of the human MDR1 gene. AAPS J 7:E1-E5.
15. Horio M, Gottesman MM, Pastan I. 1988. ATP-dependent transport of vinblastine in vesicles from human multidrug-resistant cells. Proc Natl Acad Sci USA 85:3580-3584.
16. Johne A, Kopke K, Gerloff T, Mai I, Rietbrock S, Meisel C, Hoffmeyer S, Kerb R, Fromm MF, Brinkmann U, Eichelbaum M, Brockmoller J, Cascorbi I, Roots I. 2002. Modulation of steady-state kinetics of digoxin by haplotypes of the P-glycoprotein MDR1 gene. Clin Pharmacol Ther 72:584-594.
17. Kroetz DL, Pauli-Magnus C, Hodges LM, Huang CC, Kawamoto M, Johns SJ, Stryke D, Ferrin TE, DeYoung J, Taylor T, Carlson EJ, Herskowitz I, Giacomini KM, Clark AG. 2003. Sequence diversity and haplotype structure in the human ABCB1 (MDR1, multidrug resistance transporter) gene. Pharmacogenetics 13:481-494.