Evaluation of the titanium dioxide approach for MS analysis of phosphopeptides

Journal of Mass Spectrometry

Volumn 41, Issue 12, 2006, Pages 1623-1632

  Klemm, Clementine ^a   Otto, Sebastian ^a   Wolf, Constanze ^a   Haseloff, Reiner F ^a   Beyermann, Michael ^a   Krause, Eberhard ^a  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

Author keywords

Electrospray ionization; Matrix assisted laser desorption ionization; Phosphopeptides; Tandem mass spectrometry; Titanium dioxide microcolumn

Indexed keywords

AMINO ACIDS; HYDROPHOBICITY; MASS SPECTROMETRY; PHOSPHATES; SYNTHESIS (CHEMICAL); TITANIUM DIOXIDE;

ELECTROSPRAY IONIZATION (ESI); MATRIX-ASSISTED LASER DESORPTION/IONIZATION MALDI); PHOSPHOPEPTIDES; TITANIUM DIOXIDE MICROCOLUMNS;

PROTEINS;

AMINO ACID; PHOSPHOPEPTIDE; TITANIUM DIOXIDE;

BINDING AFFINITY; CONFERENCE PAPER; HYDROPHOBICITY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TANDEM MASS SPECTROMETRY;

ANIMALS; CELLS, CULTURED; ENDOTHELIAL CELLS; HYDROPHOBICITY; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEOMICS; RATS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TITANIUM;

EID: 33845992932     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1129     Document Type: Conference Paper

References (35)

1. McLachlin DT, Chait BT. Analysis of phosphorylated proteins and peptides by mass spectrometry. Curr. Opin. Chem. Biol. 2001; 5:591.
2. Mann M, Ong SE, Gronborg M, Steen H, Jensen ON, Pandey A. Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome. Trends Biotechnol. 2002; 20: 261.
3. Jensen ON. Modification-specific proteomics: characterization of post-translational modifications by mass spectrometry. Curr. Opin. Chem. Biol. 2004; 8: 33.
4. Steen H, Jebanathirajah JA, Rush J, Morrice N, Kirschner MW. Phosphorylation analysis by mass spectrometry-Myths, facts, and the consequences for qualitative and quantitative measurements. Mol. Cell. Proteomics 2006; 5: 172.
5. Neubauer G, Mann M. Mapping of phosphorylation sites of gel-isolated proteins by nanoelectrospray tandem mass spectrometry: Potentials and limitations. Anal. Chem. 1999; 71: 235.
6. Steen H, Kuster B, Fernandez M, Pandey A, Mann M. Detection of tyrosine phosphorylated peptides by precursor ion scanning quadrupole TOF mass spectrometry in positive ion mode. Anal. Chem. 2001; 73: 1440.
7. Wind M, Edler M, Jakubowski N, Linscheid M, Wesch H, Lehmann WD. Analysis of protein phosphorylation by capillary liquid chromatography coupled to element mass spectrometry with P-31 detection and to electrospray mass spectrometry. Anal. Chem. 2001; 73: 29.
8. Schlosser A, Bodem J, Bossemeyer D, Grummt I, Lehmann WD. Identification of protein phosphorylation sites by combination of elastase digestion, immobilized metal affinity chromatography, and quadrupole-time of flight tandem mass spectrometry. Proteomics 2002; 2: 911.
9. Salomon AR, Ficarro SB, Brill LM, Brinker A, Phung QT, Ericson C, Sauer K, Brock A, Horn DM, Schultz PG, Peters EC. Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2003; 100: 443.
10. Posewitz MC, Tempst P. Immobilized gallium(III) affinity chromatography of phosphopeptides. Anal. Chem. 1999; 71: 2883.
11. Ficarro SB, McCleland ML, Stukenberg PT, Burke DJ, Ross MM, Shabanowitz J, Hunt DF, White FM. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 2002; 20: 301.
12. Oda Y, Nagasu T, Chait BT. Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome. Nat. Biotechnol. 2001; 19: 379.
13. Adamczyk M, Gebler JC, Wu J. Selective analysis of phosphopeptides within a protein mixture by chemical modification, reversible biotinylation and mass spectrometry. Rapid Commun. Mass Spectrom. 2001; 15: 1481.
14. Thaler F, Valsasina B, Baldi R, Jin X, Stewart A, Isacchi A, Kalisz HM, Rusconi L. A new approach to phosphoserine and phosphothreonine analysis in peptides and proteins: chemical modification, enrichment via solid-phase reversible binding, and analysis by mass spectrometry. Anal. Bioanal. Chem. 2003; 376: 366.
15. Klemm C, Schroder S, Gluckmann M, Beyermann M, Krause E. Derivatization of phosphorylated peptides with S- and N-nucleophiles for enhanced ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2004; 18: 2697.
16. Goshe MB, Conrads TP, Panisko EA, Angell NH, Veenstra TD, Smith RD. Phosphoprotein isotope-coded affinity tag approach for isolating and quantitating phosphopeptides in proteome-wide analyses. Anal. Chem. 2001; 73: 2578.
17. Weckwerth W, Willmitzer L, Fiehn O. Comparative quantification and identification of phosphoproteins using stable isotope labeling and liquid chromatography/mass spectrometry. Rapid Commun. Mass Spectrom. 2000; 14: 1677.
18. Molloy MP, Andrews PC. Phosphopeptide derivatization signatures to identify serine and threonine phosphorylated peptides by mass spectrometry. Anal. Chem. 2001; 73: 5387.
19. Steen H, Mann M. A new derivatization strategy for the analysis of phosphopeptides by precursor ion scanning in positive ion mode. J. Am. Soc. Mass Spectrom. 2002; 13: 996.
20. Sano A, Nakamura H. Chemo-affinity of titania for the column-switching HPLC analysis of phosphopeptides. Anal. Sci. 2004; 20: 565.
21. Pinkse MWH, Uitto PM, Hilhorst MJ, Ooms B, Heck AJR. Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-nanoLC-ESI-MS/MS and titanium oxide precolumns. Anal. Chem. 2004; 76: 3935.
22. Larsen MR, Thingholm TE, Jensen ON, Roepstorff P, Jorgensen TJD. Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol. Cell Proteomics 2005; 4: 873.
23. Connor PA, McQuillan AJ. Phosphate adsorption onto TiO2 from aqueous solutions: An in situ internal reflection infrared spectroscopic study. Langmuir 1999; 15:TU 2916.
24. Kawahara M, Nakamura H, Nakajima T. Group separation of ribonucleosides and deoxyribonucleosides on a new ceramic titania column. Anal. Sci. 1989; 5: 763.
25. Schlosser A, Vanselow JT, Kramer A. Mapping of phosphorylation sites by a multi-protease approach with specific phosphopeptide enrichment and nanoLC-MS/MS analysis. Anal. Chem. 2005; 77: 5243.
26. Janek K, Wenschuh H, Bienert M, Krause E. Phosphopeptide analysis by positive and negative ion matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2001; 15: 1593.
27. Haseloff RF, Krause E, Bigl M, Mikoteit K, Stanimirovic D, Blasig IE. Differential protein expression in brain capillary endothelial cells induced by hypoxia and posthypoxic reoxygenation. Proteomics 2006; 6: 1803.
28. Bantscheff M, Dumpelfeld B, Kuster B. Femtomol sensitivity post-digest O-18 labeling for relative quantification of differential protein complex composition. Rapid Commun. Mass Spectrom. 2004; 18: 869.
29. Johnson KL, Muddiman DC. A method for calculating O-16/O-18 peptide ion ratios for the relative quantification of proteomes. J. Am. Soc. Mass Spectrom. 2004; 15: 437.
30. Korbel S, Schumann M, Bittorf T, Krause E. Relative quantification of erythropoietin receptor-dependent phosphoproteins using in-gel O-18-labeling and tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2005; 19: 2259.
31. DaSilva AJ, Li ZW, DeVera C, Canto E, Findell P, Rudd CE. Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production. Proc. Natl. Acad. Sci. U.S.A. 1997; 94: 7493.
32. Griffiths EK, Penninger JM. Communication between the TCR and integrins: role of the molecular adapter ADAP/Fyb/Slap. Curr. Opin. Immunol. 2002; 14: 317.
33. Sakamoto Y, Kawakami N, Sasagawa T. Prediction of peptide retention times. J. Chromatogr. 1988; 442: 69.
34. Baumgart S, Lindner Y, Kühne R, Oberemm A, Wenschuh H, Krause E. The contributions of specific amino acid side chains to signal intensities of peptides in matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2004; 18: 863.
35. Kratzer R, Eckerskorn C, Karas M, Lottspeich F. Suppression effects in enzymatic peptide ladder sequencing using ultraviolet-matrix assisted laser desorption/ionization mass spectrometry. Electrophoresis 1998; 19: 1910.