Identification of protein phosphorylation sites by combination of elastase digestion, immobilized metal affinity chromatography, and quadrupole-time of flight tandem mass spectrometry

Proteomics

Volumn 2, Issue 7, 2002, Pages 911-918

  Schlosser, Andreas ^a   Bodem, Jochen ^b   Bossemeyer, Dirk ^c   Grummt, Ingrid ^b   Lehmann, Wolf Dieter ^a,c  

^a Central Spectroscopy ^*  (Germany)

^b Division of Molecular Biology of the Cell II

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

Elastase; Immobilized metal affinity chromatography; Protein phosphorylation; Quadrupole time of flight; Tandem mass spectrometry

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; ELASTASE; PHOSPHOPEPTIDE; PHOSPHOPROTEIN; PROTEIN; ISOENZYME; PANCREATIC ELASTASE; PROTEIN KINASE A CALPHA; RECOMBINANT PROTEIN; RRN3 PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; BINDING AFFINITY; CHROMATOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; DATA BASE; ENZYME MECHANISM; HUMAN; HUMAN CELL; MASS SPECTROMETRY; METAL BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; PHOSPHORYLATION; PROTEIN DATABASE;

CHROMATOGRAPHY, AFFINITY; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DATABASES, PROTEIN; HUMAN; ISOENZYMES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PANCREATIC ELASTASE; PHOSPHOPROTEINS; PHOSPHORYLATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRANSCRIPTION FACTORS; HUMANS;

EID: 0035997277     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/1615-9861(200207)2:7<911::AID-PROT911>3.0.CO;2-K     Document Type: Conference Paper

References (36)