The gas-phase absorption spectrum of a neutral GFP model chromophore

Biophysical Journal

Volumn 92, Issue 1, 2007, Pages 201-207

  Lammich, L ^a   Petersen, M Åxman ^b   Nielsen, M Brøndsted ^b   Andersen, L H ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; GREEN FLUORESCENT PROTEIN;

ABSORPTION SPECTROSCOPY; ARTICLE; CHEMICAL STRUCTURE; CHROMATOPHORE; DATA ANALYSIS; SPECTRUM;

EID: 33845992867     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.106.093674     Document Type: Article

References (39)

1. Wald, G. 1968. Molecular basis of visual excitation. Science. 162:230-239.
2. Andersen, L. H., I. B. Nielsen, M. B. Kristensen, M. O. A. E. Ghazaly, S. Haacke, M. B. Nielsen, and M. Petersen. 2005. Absorption of Schiff-base retinal chromophores in vacuo. J. Am. Chem. Soc. 127:12347-12350.
3. Tsien, R. Y. 1998. The green fluorescent protein. Annu. Rev. Biochem. 67:509-544.
4. Zimmer, M. 2002. Green fluorescent protein (GFP): applications, structure, and related photophysical behavior. Chem. Rev. 102:759-782.
5. Prendergast, F. G. 1999. Biophysics of the green fluorescent protein. Methods Cell Biol. 58:1-18.
6. Ormö, M., A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 273:1392-1395.
7. Nielsen, S. B., A. Lapierre, J. U. Andersen, U. V. Pedersen, S. Tomita, and L. H. Andersen. 2001. Absorption spectrum of the green fluorescent protein chromophore anion in vacuo. Phys. Rev. Lett. 87:228102.
8. Andersen, L. H., A. Lapierre, I. B. Nielsen, S. B. Nielsen, U. V. Pedersen, S. U. Pedersen, and S. Tomita. 2002. Photo-absorption studies of chromophores at ELISA storage ring. Nucl. Phys. Rev. 19:176-179.
9. Andersen, L. H., A. Lapierre, S. B. Nielsen, I. B. Nielsen, S. U. Pedersen, U. V. Pedersen, and S. Tomita. 2002. Chromophores of the green fluorescent protein studied in the gas phase. Eur. Phys. J. D. 20:597-600.
10. Boyé, S., S. B. Nielsen, H. Krogh, I. B. Nielsen, U. V. Pedersen, A. F. Bell, X. He, P. J. Tonge, and L. H. Andersen. 2003. Gas-phase absorption properties of DsRed model chromophores. Phys. Chem. Chem. Phys. 5:3021-3026.
11. Boyé, S., I. B. Nielsen, S. B. Nielsen, H. Krogh, A. Lapierre, H. B. Pedersen, S. U. Pedersen, U. V. Pedersen, and L. H. Andersen. 2003. Gas-phase absorption properties of a green fluorescent protein-mutant chromophore: the W7 clone. J. Chem. Phys. 119:338-345.
12. Boyé, S., H. Krogh, I. B. Nielsen, S. B. Nielsen, S. U. Pedersen, U. V. Pedersen, L. H. Andersen, A. F. Bell, X. He, and P. J. Tonge. 2003. Vibrationally resolved photoabsorption spectroscopy of red fluorescent protein chromophore anions. Phys. Rev. Lett. 90:118103.
13. Andersen, L. H., H. Bluhme, S. Boyé, T. J. D. Jørgensen, H. Krogh, I. B. Nielsen, S. B. Nielsen, and A. Svendsen. 2004. Experimental studies of the photophysics of gas-phase fluorescent protein chromophores. Phys. Chem. Chem. Phys. 6:2617-2627.
14. Nielsen, I. B., S. Boyé-Péronne, M. O. A. E. Ghazaly, M. B. Kristensen, S. B. Nielsen, and L. H. Andersen. 2005. Absorption spectra of photoactive yellow protein chromophores in vacuum. Biophys. J. 89:2597-2604.
15. Niwa, H., S. Inouye, T. Hirano, T. Matsuno, S. Kojima, M. Kubota, M. Ohashi, and F. I. Tsuji. 1996. Chemical nature of the light emitter of the Aequorea green fluorescent protein. Proc. Natl. Acad. Sci. USA. 93:13617-13622.
16. Toniolo, A., S. Olsen, L. Manoha, and T. Martinez. 2004. Conical intersection dynamics in solution: the chromophore of green fluorescent protein. Faraday Discuss. 127:149-163.
17. Jaye, A. A., D. Stoner-Ma, P. Matousek, M. Towrie, P. J. Tonge, and S. R. Meech. 2006. Time-resolved emission spectra of green fluorescent protein. Photochem. Photobiol. 82:373-379.
18. Brejc, K., T. Sixma, P. Kitts, S. Kain, R. Tsien, M. Ormö, and S. Remington. 1997. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. USA. 94:2306-2311.
19. Ghose, M., S. Mandal, D. Roy, R. Manda, and G. Basu. 2001. Dielectric relaxation in a single tryptophan protein. FEBS Lett. 509:337-340.
20. Cohen, B. E., T. B. McAnaney, E. S. Park, Y. N. Jan, S. G. Boxer, and L. Y. Jan. 2002. Probing protein electrostatics with a synthetic fluorescent amino acid. Science. 296:1700-1703.
21. Weber, W., V. Helms, J. A. McCammon, and P. W. Langhoff. 1999. Shedding light on the dark and weakly fluorescent states of green fluorescent proteins. Proc. Natl. Acad. Sci. USA. 96:6177-6182.
22. Voityuk, A. A., M.-E. Michel-Beyerle, and N. Rösch. 1998. Quantum chemical modeling of structure and absorption spectra of the chromophore in green fluorescent proteins. Chem. Phys. 231:13-25.
23. Helms, V. 2002. Electronic excitations of biomolecules studied by quantum chemistry. Curr. Opin. Struct. Biol. 12:169-175.
24. Marques, M. A. L., X. López, D. Varsano, A. Castro, and A. Rubio. 2003. Time-dependent density-functional approach for biological chromophores: the case of the green fluorescent protein. Phys. Rev. Lett. 90:258101.
25. Martin, M. E., F. Negri, and M. Olivucci. 2004. Origin, nature, and fate of the fluorescent state of the green fluorescent protein chromophore at the CASPT2//CASSCF resolution. J. Am. Chem. Soc. 126:5452-5464.
26. Sinicropi, A., T. Andruniow, N. Ferre, R. Basosi, and M. Olivucci. 2005. Properties of the emitting state of the green fluorescent protein resolved at the CASPT2//CASSCF/CHARMM level. J. Am. Chem. Soc. 127:11534-11535.
27. Kojima, S., H. Ohkawa, T. Hirano, S. Maki, H. Niwa, M. Ohashi, S. Inouye, and F. I. Tsuji. 1998. Fluorescent properties of model chromophores of tyrosine-66 substituted mutants of Aequorea green fluorescent protein (GFP). Tetrahedron Lett. 39:5239-5242.
28. Møller, S. P. 1997. ELISA, an electrostatic storage ring for atomic physics. Nucl. Instrum. Meth. Phys. Res. A. 394:281-286.
29. Andersen, L. H., O. Heber, and D. Zajfman. 2004. Physics with electrostatic rings and traps. J. Phys. B. 37:R57-R88.
30. Andersen, J. U., E. Bonderup, and K. Hansen. 2001. On the concept of temperature for a small isolated system. J. Chem. Phys. 114:6518-6525.
31. Andersen, J. U., P. Hvelplund, S. B. Nielsen, S. Tomita, H. Wahlgreen, S. P. Møller, U. V. Pedersen, J. S. Forster, and T. J. D. Jørgensen. 2002. The combination of an electrospray ion source and an electrostatic storage ring for lifetime and spectroscopy experiments on biomolecules. Rev. Sci. Instrum. 73:1284-1287.
32. Gerlich, D. 1993. Experimental investigations of ion-molecule reactions relevant to interstellar chemistry. J. Chem. Soc. Faraday Trans. 89:2199-2208.
33. Morise, H., O. Shimomura, F. H. Johnson, and J. Winant. 1974. Intermolecular energy-transfer in bioluminescent systems of Aequorea. Biochemistry. 13:2656-2662.
34. Bonsma, S., R. Purchase, S. Jezowski, J. Gallus, F. Konz, and S. Volker. 2005. Green and red fluorescent proteins: photo- and thermally induced dynamics probed by site-selective spectroscopy and hole burning. ChemPhysChem. 6:838-849.
35. Frisch, M. J., G. W. Trucks, H. B. Schlegel, G. E. Scuseria, M. A. Robb, J. R. Cheeseman, J. A. Montgomery, Jr., T. Vreven, K. N. Kudin, J. C. Burant, J. M. Millam, S. S. Iyengar, J. Tomasi, V. Barone, B. Mennucci, M. Cossi, G. Scalmani, N. Rega, G. A. Petersson, H. Nakatsuji, M. Hada, M. Ehara, K. Toyota, R. Fukuda, J. Hasegawa, M. Ishida, T. Nakajima, Y. Honda, O. Kitao, H. Nakai, M. Klene, X. Li, J. E. Knox, H. P. Hratchian, J. B. Cross, V. Bakken, C. Adamo, J. Jaramillo, R. Gomperts, R. E. Stratmann, O. Yazyev, A. J. Austin, R. Cammi, C. Pomelli, J. W. Ochterski, P. Y. Ayala, K. Morokuma, G. A. Voth, P. Salvador, J. J. Dannenberg, V. G. Zakrzewski, S. Dapprich, A. D. Daniels, M. C. Strain, O. Farkas, D. K. Malick, A. D. Rabuck, K. Raghavachari, J. B. Foresman, J. V. Ortiz, Q. Cui, A. G. Baboul, S. Clifford, J. Cioslowski, B. B. Stefanov, G. Liu, A. Liashenko, P. Piskorz, I. Komaromi, R. L. Martin, D. J. Fox, T. Keith, M. A. Al-Laham, C. Y. Peng, A. Nanayakkara, M. Challacombe, P. M. W. Gill, B. Johnson, W. Chen, M. W. Wong, C. Gonzalez, and J. A. Pople. 2004. Gaussian 03, Revision C.02. Gaussian, Inc., Wallingford, CT.
36. Voityuk, A. A., A. D. Kummer, M.-E. Michel-Beyerle, and N. Rösch. 2001. Absorption spectra of the GFP chromophore in solution: comparison of theoretical and experimental results. Chem. Phys. 269:83-91.
37. Marques, M. A. L., X. Lopez, D. Varsano, A. Castro, and A. Rubio. 2003. Time-dependent density-functional approach for biological chromophores: the case of the green fluorescent protein. Phys. Rev. Lett. 90:258101.
38. Havranek, J. J., and P. B. Harbury. 1999. Tanford-Kirkwood electrostatics for protein modeling. Proc. Natl. Acad. Sci. USA. 96:11145-11150.
39. Nielsen, I. B., M. Å. Petersen, L. Lammich, M. B. Nielsen, and L. H. Andersen. 2006. Absorption studies of neutral retinal Schiff Base chromophores. To appear in J. Phys. Chem. A.