Efficient expression of recombinant human monoclonal antibodies in Drosophila S2 cells

Journal of Immunological Methods

Volumn 318, Issue 1-2, 2007, Pages 37-46

  Johansson, Daniel X ^a   Drakenberg, Katarina ^a   Hopmann, Kathrin H ^a   Schmidt, Alexej ^b   Yari, Fayezeh ^a   Hinkula, Jorma ^c   Persson, Mats A A ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c LINKÖPING UNIVERSITY   (Sweden)

Author keywords

Expression vector; Ig heavy chain; Phage display; Single chain Fv fragment

Indexed keywords

COMPLEMENTARY DNA; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G1; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MONOCLONAL ANTIBODY;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; DROSOPHILA; EXPRESSION VECTOR; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CELL LINE; CELL LINE, TUMOR; DROSOPHILA; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENETIC VECTORS; HIV-1; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; RECOMBINANT PROTEINS; TRANSFECTION; VIRAL ENVELOPE PROTEINS;

MAMMALIA;

EID: 33845896262     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.08.017     Document Type: Article

References (32)

1. Adachi A., Gendelman H.E., Koenig S., Folks T., Willey R., Rabson A., and Martin M.A. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J. Virol. 59 (1986) 284
2. Allander T., Drakenberg K., Beyene A., Rosa D., Abrignani S., Houghton M., Widell A., Grillner L., and Persson M.A.A. Recombinant human monoclonal antibodies against different conformational epitopes of the E2 envelope glycoprotein of hepatitis C virus that inhibit its interaction with CD81. J. Gen. Virol. 81 (2000) 2451
3. Ames R.S., Tornetta M.A., Deen K., Jones C.S., Swift A.M., and Ganguly S. Conversion of murine Fabs isolated from a combinatorial phage display library to full length immunoglobulins. J. Immunol. Methods 184 (1995) 177
4. Barbas III C.F., and Wagner J. Synthetic human antibodies: selecting and evolving functional proteins. Methods, Comput. Methods Enzymol. 8 (1995) 94
5. Barbas III C.F., Kang A.S., Lerner R.A., and Benkovic S.J. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 7978
6. Bebbington C.R., Renner G., Thomson S., King D., Abrams D., and Yarranton G.T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Biotechnology (N Y) 10 (1992) 169
7. Berglund C.M., Radesater A.C., Persson M.A.A., and Budd Haeberlein S.L. UV-induced apoptosis in SH-SY5Y cells: contribution to apoptosis by JNK signaling and cytochrome c. J. Neurosci. Res. 78 (2004) 580
8. Berntzen G., Lunde E., Flobakk M., Andersen J.T., Lauvrak V., and Sandlie I. Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells. J. Immunol. Methods 298 (2005) 93
9. Boyd P.N., Lines A.C., and Patel A.K. The effect of the removal of sialic acid, galactose and total carbohydrate on the functional activity of Campath-1H. Mol. Immunol. 32 (1995) 1311
10. Devito C., Levi M., Broliden K., and Hinkula J. Epitope-mapping of B-cell epitopes in rabbits immunized with various gag antigens for the induction of HIV-1 gag capture ELISA reagents. J. Immunol. Methods 238 (2000) 69
11. Felber B.K., Drysdale C.M., and Pavlakis G.N. Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein. J. Virol. 64 (1990) 3734
12. Griffiths A.D. Production of human antibodies using bacteriophage. Curr. Opin. Immunol. 5 (1993) 263
13. Guay L.A., Musoke P., Hom D.L., Nakabiito C., Bagenda D., Fletcher C.V., Marum L.H., Fowler M.G., Falksveden L.G., Wahren B., Kataaha P., Wigzell H., Mmiro F.A., and Jackson J.B. Phase I/II trial of HIV-1 hyperimmune globulin for the prevention of HIV-1 vertical transmission in Uganda. AIDS 16 10 (2002) 1391
14. Guha M. Monoclonal antibodies: magic bullets close in on target. Financial Times FT.com 2005; October 18.
15. Guttieri M.C., Bookwalter C., and Schmaljohn C. Expression of a human, neutralizing monoclonal antibody specific to Puumala virus G2-protein in stably-transformed insect cells. J. Immunol. Methods 246 (2000) 97
16. Hasemann C.A., and Capra J.D. High-level production of a functional immunoglobulin heterodimer in a baculovirus expression system. Proc. Natl. Acad. Sci. U. S. A. 87 (1990) 3942
17. Hill D.R., Aumiller J.J., Shi X., and Jarvis D.L. Isolation and analysis of a baculovirus vector that supports recombinant glycoprotein sialylation by SfSWT-1 cells cultured in serum-free medium. Biotechnol. Bioeng. 95 1 (2006) 37
18. Horwitz A.H., Chang C.P., Better M., Hellstrom K.E., and Robinson R.R. Secretion of functional antibody and Fab fragment from yeast cells. Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 8678
19. Jung S., Spinelli S., Schimmele B., Honegger A., Pugliese L., Cambillau C., and Pluckthun A. The importance of framework residues H6, H7 and H10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody V(H) domains. J. Mol. Biol. 309 (2001) 701
20. Kipriyanov S.M., Moldenhauer G., Martin A.C., Kupriyanova O.A., and Little M. Two amino acid mutations in an anti-human CD3 single chain Fv antibody fragment that affect the yield on bacterial secretion but not the affinity. Protein Eng. 10 (1997) 445
21. Kirkpatrick R.B., Ganguly S., Angelichio M., Griego S., Shatzman A., Silverman C., and Rosenberg M. Heavy chain dimers as well as complete antibodies are efficiently formed and secreted from Drosophila via a BiP-mediated pathway. J. Biol. Chem. 270 (1995) 19800
22. Koles K., Irvine K.D., and Panin V.M. Functional characterization of Drosophila sialyltransferase. J. Biol. Chem. 279 (2004) 4346
23. Lavillette D., Tarr A.W., Voisset C., Donot P., Bartosch B., Bain C., Patel A.H., Dubuisson J., Ball J.K., and Cosset F.L. Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus. Hepatology 41 (2005) 265
24. Persson M.A.A., Caothien R.H., and Burtonl D.R. Generation of diverse high affinity human monoclonal antibodies by repertoire cloning. Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 2432
25. Rader C., and Barbas III C.F. Phage display of combinatorial antibody libraries. Curr. Opin. Biotechnol. 8 (1997) 503
26. Raju T.S., Lerner L., and O'Connor J.V. Glycopinion: biological significance and methods for the analysis of complex carbohydrates of recombinant glycoproteins. Biotechnol. Appl. Biochem. 24 (1996) 191
27. Roth J., Kempf A., Reuter G., Schauer R., and Gehring W.J. Occurrence of sialic acids in Drosophila melanogaster. Science 256 (1992) 673
28. Samuelsson A., Chiodi F., Öhman P., Putkonen P., Norrby E., and Persson M.A.A. Chimeric Macaque/Human Fab Molecules Neutralize Simian Immunodeficiency Virus. Virology 207 (1995) 495
29. Samuelsson A., Yari F., Hinkula J., Ersoy O., Norrby E., and Persson M.A.A. Human antibodies from phage libraries: neutralizing activity against human immunodeficiency virus type 1 equally improved after expression as Fab and IgG in mammalian cells. Eur. J. Immunol. 26 (1996) 3029
30. Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morph. 27 (1972) 363
31. Sundqvist V.-A., Albert J., Ohlsson E., Hinkula J., Fenyö E.-M., and Wahren B. HIV-1 p24 antigen variation in tissue culture of isolates with defined growth characteristics. J. Virol. 29 (1989) 170
32. Welschof M., Terness P., Kipriyanov S.M., Stanescu D., Breitling F., Dorsam H., Dubel S., Little M., and Opelz G. The antigen-binding domain of a human IgG-anti-F(ab′)2 autoantibody. Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 1902