Structural basic for ribosome recruitment and manipulation by a viral IRES RNA

Science

Volumn 314, Issue 5804, 2006, Pages 1450-1454

  Pfingsten, Jennifer S ^a   Costantino, David A ^a   Kieft, Jeffrey S ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; ELECTRON MICROSCOPY; MOLECULAR STRUCTURE; PROTEINS;

DICISTROVIRIDAE INTERGENIC REGION; INTERNAL RIBOSOMAL ENTRY SITES (IRES); RNA TRANSLATION INITIATION APPARATUS;

RNA;

CIS ACTING ELEMENT; SPACER DNA; VIRUS RNA;

CHROMOSOME; GENETIC ANALYSIS; PROTEIN; RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; COMPLEX FORMATION; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; INTERNAL RIBOSOME ENTRY SITE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; RNA STRUCTURE; RNA TRANSLATION; TRANSLATION INITIATION; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; CRYSTALLIZATION; GENETICS; METABOLISM; MUTATION; PROTEIN SYNTHESIS; REGULATORY RNA SEQUENCE; RIBOSOME; RNA VIRUS; X RAY CRYSTALLOGRAPHY;

DICISTROVIRIDAE;

BINDING SITES; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MUTATION; NUCLEIC ACID CONFORMATION; PROTEIN BIOSYNTHESIS; REGULATORY SEQUENCES, RIBONUCLEIC ACID; RIBOSOMES; RNA VIRUSES; RNA, VIRAL;

EID: 33845335508     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1133281     Document Type: Article

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28. We acknowledge the staff at beamline ALS 8.2.1 for assistance, R. Zhao for managing the University of Colorado (UC) at Denver and Health Sciences Center x-ray facility, D. Farrell for computer administration, and M. Churchill, R. Batey, and A. Ferré-D'Amaré for useful discussions and advice. We especially thank C. Spahn for supplying various cryo-EM density files and R. Batey for the iridium (III) hexammine. R. Batey, M. Churchill, O. Bentley, L. Krushel, and R. Zhao provided a critical reading of this manuscript. This work was supported by a grant from NIH and funding from the UC Cancer Center in support of the x-ray facility. Structure factors and coordinates have been deposited in the Protein Data Bank under accession code 2IL9.