A single mutation at Tyr143 of human S-adenosylhomocysteine hydrolase renders the enzyme thermosensitive and affects the oxidation state of bound cofactor nicotinamide-adenine dinucleotide

Biochemical Journal

Volumn 400, Issue 2, 2006, Pages 245-253

  Belužić, Robert ^a   Cuk, Mario ^b   Pavkov, Tea ^c   Fumić, Ksenija ^d   Barić, Ivo ^b   Mudd, S Harvey ^e   Jurak, Igor ^f   Vugrek, Oliver ^a  

^a RUDER BO KOVI INSTITUTE   (Croatia)

^b UNIVERSITY OF ZAGREB   (Croatia)

^c UNIVERSITY OF GRAZ   (Austria)

^d KBC Zagreb   (Croatia)

^e NATIONAL INSTITUTE OF MENTAL HEALTH   (United States)

^f ROBERT KOCH INSTITUTE   (Germany)

Author keywords

5,5 dithiobis (2 nitrobenzoic acid) (DTNB); CD; Hydrogen bond; In vitro mutagenesis; S adenosylhomocysteine hydrolase (AdoHcyase) deficiency; Thermosensitivity

Indexed keywords

CATALYSTS; ENZYME KINETICS; GENES; HYDROGEN BONDS; HYDROLYSIS; MOLECULAR STRUCTURE; OXIDATION; SYNTHESIS (CHEMICAL);

ENZYME STABILITY; HYDROLASE; QUANTITATIVE BINDING; THERMOSENSITIVITY;

PROTEINS;

ADENOSYLHOMOCYSTEINASE; GLUTAMIC ACID; LYSINE; NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCING AGENT; TRYPTOPHAN; TYROSINE; CYSTEINE; DITHIOTHREITOL; RECOMBINANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; DISULFIDE BOND; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; GENE MUTATION; HEAT SENSITIVITY; HYDROGEN BOND; HYDROLYSIS; MISSENSE MUTATION; OXIDATION; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN QUATERNARY STRUCTURE; STOP CODON; TEMPERATURE SENSITIVE MUTANT; CATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HEAT; HUMAN; KINETICS; METABOLISM; MUTATION; OXIDATION REDUCTION REACTION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ADENOSYLHOMOCYSTEINASE; AMINO ACID SUBSTITUTION; CATALYSIS; CYSTEINE; DITHIOTHREITOL; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; HUMANS; HYDROGEN BONDING; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; NAD; OXIDATION-REDUCTION; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS; REDUCING AGENTS; STRUCTURE-ACTIVITY RELATIONSHIP; TYROSINE;

EID: 33845210840     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20060749     Document Type: Article

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