Purification and characterization of the guinea pig sigma-1 receptor functionally expressed in Escherichia coli

Protein Expression and Purification

Volumn 51, Issue 2, 2007, Pages 283-292

  Ramachandran, Subramaniam ^a   Lu, Hongliang ^a   Prabhu, Usha ^a   Ruoho, Arnold E ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Maltose binding protein; Methamphetamine; Pentazocine; Protein purification; Sigma receptor

Indexed keywords

BLOOD CLOTTING FACTOR 10A; CARRIER PROTEIN; HYBRID PROTEIN; LIGAND; MALTOSE BINDING PROTEIN; PENTAZOCINE; SIGMA 1 OPIATE RECEPTOR; SIGMA OPIATE RECEPTOR; SIGMA-1 RECEPTOR;

ANIMAL; ARTICLE; DRUG STABILITY; ESCHERICHIA COLI; GENETICS; GUINEA PIG; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM;

ANIMALS; CARRIER PROTEINS; DRUG STABILITY; ESCHERICHIA COLI; FACTOR XA; GUINEA PIGS; LIGANDS; MALTOSE-BINDING PROTEINS; PENTAZOCINE; RECEPTORS, SIGMA; RECOMBINANT FUSION PROTEINS;

CAVIA PORCELLUS; ESCHERICHIA COLI;

EID: 33751399402     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.07.019     Document Type: Article

References (35)

1. Martin W.R., Eades C.G., Thompson J.A., Huppler R.E., and Gilbert P.E. The effects of morphine- and nalorphine- like drugs in the nondependent and morphine-dependent chronic spinal dog. J. Pharmacol. Exp. Ther. 197 (1976) 517-532
2. Hanner M., Moebius F.F., Flandorfer A., Knaus H.G., Striessnig J., Kempner E., and Glossmann H. Purification, molecular cloning, and expression of the mammalian sigma1-binding site. Proc. Natl. Acad. Sci. USA 93 (1996) 8072-8077
3. Nguyen E.C., McCracken K.A., Liu Y., Pouw B., and Matsumoto R.R. Involvement of sigma (sigma) receptors in the acute actions of methamphetamine: receptor binding and behavioral studies. Neuropharmacology 49 (2005) 638-645
4. Aydar E., Palmer C.P., Klyachko V.A., and Jackson M.B. The sigma receptor as a ligand-regulated auxiliary potassium channel subunit. Neuron 34 (2002) 399-410
5. Hayashi T., and Su T.P. Regulating ankyrin dynamics: roles of sigma-1 receptors. Proc. Natl. Acad. Sci. USA 98 (2001) 491-496
6. Ela C., Barg J., Vogel Z., Hasin Y., and Eilam Y. Sigma receptor ligands modulate contractility, Ca++ influx and beating rate in cultured cardiac myocytes. J. Pharmacol. Exp. Ther. 269 (1994) 1300-1309
7. Spruce B.A., Campbell L.A., McTavish N., Cooper M.A., Appleyard M.V., O'Neill M., Howie J., Samson J., Watt S., Murray K., McLean D., Leslie N.R., Safrany S.T., Ferguson M.J., Peters J.A., Prescott A.R., Box G., Hayes A., Nutley B., Raynaud F., Downes C.P., Lambert J.J., Thompson A.M., and Eccles S. Small molecule antagonists of the sigma-1 receptor cause selective release of the death program in tumor and self-reliant cells and inhibit tumor growth in vitro and in vivo. Cancer Res. 64 (2004) 4875-4886
8. Romieu P., Martin-Fardon R., Bowen W.D., and Maurice T. Sigma 1 receptor-related neuroactive steroids modulate cocaine-induced reward. J. Neurosci. 23 (2003) 3572-3576
9. Lupardus P.J., Wilke R.A., Aydar E., Palmer C.P., Chen Y., Ruoho A.E., and Jackson M.B. Membrane-delimited coupling between sigma receptors and K+ channels in rat neurohypophysial terminals requires neither G-protein nor ATP. J. Physiol. 526 Pt. 3 (2000) 527-539
10. Hong W., and Werling L.L. Evidence that the sigma(1) receptor is not directly coupled to G proteins. Eur. J. Pharmacol. 408 (2000) 117-125
11. Hayashi T., and Su T.P. Intracellular dynamics of sigma-1 receptors (sigma(1) binding sites) in NG108-15 cells. J. Pharmacol. Exp. Ther. 306 (2003) 726-733
12. Hayashi T., and Su T.P. Sigma-1 receptors (sigma(1) binding sites) form raft-like microdomains and target lipid droplets on the endoplasmic reticulum: roles in endoplasmic reticulum lipid compartmentalization and export. J. Pharmacol. Exp. Ther. 306 (2003) 718-725
13. Hayashi T., and Su T.P. Sigma-1 receptors at galactosylceramide-enriched lipid microdomains regulate oligodendrocyte differentiation. Proc. Natl. Acad. Sci. USA 101 (2004) 14949-14954
14. Langa F., Codony X., Tovar V., Lavado A., Gimenez E., Cozar P., Cantero M., Dordal A., Hernandez E., Perez R., Monroy X., Zamanillo D., Guitart X., and Montoliu L. Generation and phenotypic analysis of sigma receptor type I (sigma 1) knockout mice. Eur. J. Neurosci. 18 (2003) 2188-2196
15. Cendan C.M., Pujalte J.M., Portillo-Salido E., Montoliu L., and Baeyens J.M. Formalin-induced pain is reduced in sigma(1) receptor knockout mice. Eur. J. Pharmacol. 511 (2005) 73-74
16. Hellewell S.B., Bruce A., Feinstein G., Orringer J., Williams W., and Bowen W.D. Rat liver and kidney contain high densities of sigma 1 and sigma 2 receptors: characterization by ligand binding and photoaffinity labeling. Eur. J. Pharmacol. 268 (1994) 9-18
17. Vilner B.J., John C.S., and Bowen W.D. Sigma-1 and sigma-2 receptors are expressed in a wide variety of human and rodent tumor cell lines. Cancer Res. 55 (1995) 408-413
18. Matsumoto R.R., Liu Y., Lerner M., Howard E.W., and Brackett D.J. Sigma receptors: potential medications development target for anti-cocaine agents. Eur. J. Pharmacol. 469 (2003) 1-12
19. Su T.P., and Hayashi T. Understanding the molecular mechanism of sigma-1 receptors: towards a hypothesis that sigma-1 receptors are intracellular amplifiers for signal transduction. Curr. Med. Chem. 10 (2003) 2073-2080
20. Aydar E., Palmer C.P., and Djamgoz M.B. Sigma receptors and cancer: possible involvement of ion channels. Cancer Res. 64 (2004) 5029-5035
21. Hayashi T., and Su T.P. Sigma-1 receptor ligands: potential in the treatment of neuropsychiatric disorders. CNS Drugs 18 (2004) 269-284
22. Guitart X., Codony X., and Monroy X. Sigma receptors: biology and therapeutic potential. Psychopharmacology (Berl) 174 (2004) 301-319
23. 125I)iodoazidococaine, a photoaffinity label for the haloperidol-sensitive sigma receptor. Proc. Natl. Acad. Sci. USA 89 (1992) 1393-1397
24. Ganapathy M.E., Prasad P.D., Huang W., Seth P., Leibach F.H., and Ganapathy V. Molecular and ligand-binding characterization of the sigma-receptor in the Jurkat human T lymphocyte cell line. J. Pharmacol. Exp. Ther. 289 (1999) 251-260
25. Torrence-Campbell C., and Bowen W.D. Differential solubilization of rat liver sigma 1 and sigma 2 receptors: retention of sigma 2 sites in particulate fractions. Eur. J. Pharmacol. 304 (1996) 201-210
26. Bruns R.F., Lawson-Wendling K., and Pugsley T.A. A rapid filtration assay for soluble receptors using polyethylenimine-treated filters. Anal. Biochem. 132 (1983) 74-81
27. Salacinski P.R., McLean C., Sykes J.E., Clement-Jones V.V., and Lowry P.J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal. Biochem. 117 (1981) 136-146
28. Cheng Y., and Prusoff W.H. Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22 (1973) 3099-3108
29. Thiriot D.S., Sievert M.K., and Ruoho A.E. Identification of human vesicle monoamine transporter (VMAT2) lumenal cysteines that form an intramolecular disulfide bond. Biochemistry 41 (2002) 6346-6353
30. Maina C.V., Riggs P.D., Grandea III A.G., Slatko B.E., Moran L.S., Tagliamonte J.A., McReynolds L.A., and Guan C.D. An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein. Gene 74 (1988) 365-373
31. Miroux B., and Walker J.E. Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J. Mol. Biol. 260 (1996) 289-298
32. Moebius F.F., Reiter R.J., Hanner M., and Glossmann H. High affinity of sigma 1-binding sites for sterol isomerization inhibitors: evidence for a pharmacological relationship with the yeast sterol C8-C7 isomerase. Br. J. Pharmacol. 121 (1997) 1-6
33. Ramamoorthy J.D., Ramamoorthy S., Mahesh V.B., Leibach F.H., and Ganapathy V. Cocaine-sensitive sigma-receptor and its interaction with steroid hormones in the human placental syncytiotrophoblast and in choriocarcinoma cells. Endocrinology 136 (1995) 924-932
34. Kapust R.B., and Waugh D.S. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8 (1999) 1668-1674
35. de Costa B.R., Bowen W.D., Hellewell S.B., Walker J.M., Thurkauf A., Jacobson A.E., and Rice K.C. Synthesis and evaluation of optically pure [3H]-(+)-pentazocine, a highly potent and selective radioligand for sigma receptors. FEBS Lett. 251 (1989) 53-58