Time-dependent folding of immunological epitopes of the human chorionic gonadotropin β-subunit

Molecular and Cellular Endocrinology

Volumn 260-262, Issue , 2007, Pages 12-22

  Roig, J ^a   Krause, J M ^a   Berger, P ^b   Merz, Wolfgang E ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

Author keywords

Epitope mapping; Glycoprotein hormone subunit; hCG biosynthesis; hCG subunit; hCG core fragment

Indexed keywords

CHORIONIC GONADOTROPIN BETA SUBUNIT; CHORIONIC GONADOTROPIN BETA1; CHORIONIC GONADOTROPIN BETA2; EPITOPE; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG;

ANTIGEN EXPRESSION; ANTIGEN STRUCTURE; ARTICLE; CONTROLLED STUDY; DOWN SYNDROME; EPITOPE MAPPING; FRAGMENTATION REACTION; HUMAN; HUMAN CELL; PREGNANCY; PRIORITY JOURNAL; PROTEIN FOLDING; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; TUMOR;

ANTIBODIES, MONOCLONAL; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; DIMERIZATION; EPITOPES; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SUBUNITS; TIME FACTORS;

EID: 33751211226     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mce.2005.10.030     Document Type: Article

References (51)

1. Bedows E., Norton S.E., Huth J.R., Suganuma N., Boime I., et al. Misfolded human chorionic gonadotropin beta subunits are secreted from transfected Chinese hamster ovary cells. J. Biol. Chem. 269 (1994) 10574-10580
2. Beebe J.S., Mountjoy K., Krzesicki R.F., Perini F., and Ruddon R.W. Role of disulfide bond formation in the folding of human chorionic gonadotropin beta subunit into an alpha beta dimer assembly-competent form. J. Biol. Chem. 265 (1990) 312-317
3. Berger P., Bidart J.M., Delves P.S., Dirnhofer S., Hoermann R., et al. Immunochemical mapping of gonadotropins. Mol. Cell. Endocrinol. 125 (1996) 33-43
4. Berger P., Schwarz S., Spottl G., Wick G., and Mann K. Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies. J. Clin. Endocrinol. Metab. 77 (1993) 347-351
5. Berger P., Sturgeon C., Bidard J.M., Paus E., Gerth R., Niang M., Bristow A., Birken S., and Stenman U.H. The ISOBM TD-7 workshop on hCG and related molecules. Towards user-oriented standardization of pregnancy and tumor diagnosis: assignment of epitopes to the three-dimensional structure of diagnostically and commercially relevant monoclonal antibodies directed against human chorionic gonadotropin and derivatives. Tumor Biol. 23 (2001) 1-38
6. Birken S., Kovalevskaya G., and O'Connor J. Metabolism of hCG and hLH to multiple urinary forms. Mol. Cell. Endocrinol. 125 (1996) 121-131
7. Blithe D.L., Wehmann R.E., and Nisula B.C. Beta-core chemical and clinical properties. Trends Endocrinol. Metab. 1 (1990) 394-398
8. Bousfield G.R., Liu W.K., Sugino H., and Ward D.N. Structural studies on equine glycoprotein hormones. Amino acid sequence of equine lutropin beta-subunit. J. Biol. Chem. 262 (1987) 8610-8620
9. Bousfield G.R., Butnev V.Y., Gotschall R.R., Baker V.L., and Moore W.T. Structural features of mammalian gonadotropins. Mol. Cell. Endocrinol. 125 (1996) 3-19
10. Creighton T.E. Experimental studies of protein folding and unfolding. Progr. Biophys. Mol. Biol. 33 (1978) 231-297
11. Cole L.A. Phantom hCG and phantom choriocarcinoma. Gynecol. Oncol. 71 (1998) 325-329
12. Cole L.A., Kroll T.G., Ruddon R.W., and Hussa R.O. Differential occurrence of free beta and free alpha subunits of human chorionic gonadotropin (hCG) in pregnancy sera. J. Clin. Endocrinol. Metab. 58 (1984) 1200-1202
13. Cosgrove D.E., Campain J.A., and Cox G.S. Chorionic gonadotropin synthesis in tumor cell lines: examination of subunit accumulation, mRNA steady state levels, and gene structure. Biochim. Biophys. Acta 1007 (1989) 44-54
14. Daggett V., and Fersht A.R. Is there a unifying mechanism for protein folding?. Trends Biochem. Sci. 28 (2003) 18-25
15. Darling R.J., Ruddon R.W., and Bedows E. Intracellular folding pathway of the cystine knot-containing glycoprotein hormone α-subunit. Biochemistry 40 (2001) 577-585
16. Eaton W.A., Munoz V., Hagen S.T., Jas G.S., Lapidus L.J., Henry E.R., and Hofrichter J. Fast kinetics and mechanisms in protein folding. Annu. Rev. Biomol. Struct. 29 (2000) 327-359
17. Feng W., Huth J.R., Norton S.E., and Ruddon R.W. Asparagine-linked oligosaccharides facilitate human chorionic gonadotropin beta-subunit folding but not assembly of prefolded beta with alpha. Endocrinology 136 (1995) 52-61
18. Feng W., Matzuk M.M., Mountjoy K., Bedows E., Ruddon R.W., et al. The asparagine-linked oligosaccharides of the human chorionic gonadotropin beta subunit facilitate correct disulfide bond pairing. J. Biol. Chem. 270 (1995) 11851-11859
19. Furuhashi M., Ando H., Bielinska M., Pixley M.R., Shikone T., et al. Mutagenesis of cysteine residues in the human gonadotropin alpha subunit. Roles of individual disulfide bonds in secretion, assembly, and biologic activity. J. Biol. Chem. 269 (1994) 25543-25548
20. Hogg P.J. Disulfide bonds as switches for protein function. Trends Biochem. Sci. 28 (2003) 210-214
21. Hussa R.O. Clinical utility of human chorionic gonadotropin and alpha-subunit measurements. Obstet. Gynecol. 60 (1982) 1-12
22. Huth J.R., Perini F., Lockridge O., Bedows E., and Ruddon R.W. Protein folding and assembly in vitro parallel intracellular folding and assembly. Catalysis of folding and assembly of the human chorionic gonadotropin alpha beta dimer by protein disulfide isomerase. J. Biol. Chem. 268 (1993) 16472-16482
23. Jackson A.M., Berger P., Pixley M., Klein C., Hsueh A.J., et al. The biological action of choriogonadotropin is not dependent on the complete native quaternary interactions between the subunits. Mol. Endocrinol. 13 (1999) 2175-2188
24. Jain M., Evans M.S., King J., and Clark P.L. Monoclonal antibody epitope mapping describes tailspike β-helix folding and aggregation intermediates. J. Biol. Chem. 280 (2005) 23032-23040
25. Ji I., and Ji T.H. Differential interactions of human choriogonadotropin and its antagonistic aglycosylated analog with their receptor. Proc. Natl. Acad. Sci. U.S.A. 87 (1990) 4396-4400
26. Ji I., Okada Y., Nishimura R., and Ji T.H. Photoaffinity labeling of the choriogonadotropin receptor. Pharmacol. Ther. 43 (1989) 333-343
27. Kanda M., Jablonka-Shariff A., Sato A., Pixley M.R., Bos E., et al. Genetic fusion of an alpha-subunit gene to the follicle-stimulating hormone and chorionic gonadotropin-beta subunit genes: production of a bifunctional protein. Mol. Endocrinol. 13 (1999) 1873-1881
28. Konrad M., and Merz W.E. Regulation of N-glycosylation. Long term effect of cyclic AMP mediates enhanced synthesis of the dolichol pyrophosphate core oligosaccharide. J. Biol. Chem. 269 (1994) 8659-8666
29. Lapthorn A.J., Harris D.C., Littlejohn A., Lustbader J.W., Canfield R.E., et al. Crystal structure of human chorionic gonadotropin [see comments]. Nature 369 (1994) 455-461
30. Mann K., and Karl H.J. Molecular heterogeneity of human chorionic gonadotropin and its subunits in testicular cancer. Cancer 52 (1983) 654-660
31. Mann K., and Siddle K. Evidence for free beta-subunit secretion in so-called human chorionic gonadotropin-positive seminoma. Cancer 62 (1988) 2378-2382
32. Merz W.E. Biosynthesis of human chorionic gonadotropin: a review. Eur. J. Endocrinol. 135 (1996) 269-284
33. Merz W.E. Studies of the specific role of the subunits of choriogonadotropin for biological, immunological and physical properties of the hormone. Digestion of the alpha-subunit with carboxypeptidase A. Eur. J. Biochem. 101 (1979) 541-553
34. Munro A.J., Nielsen O.S., Duncan W., Sturgeon J., Gospodarowicz M.K., Malkin A., Thomas G.M., and Jewett M.A. An assessment of combined tumour markers in patients with seminoma: placental alkaline phosphatase (PLAP), lactate dehydrogenase (LD) and beta human chorionic gonadotrophin (beta HCG). Br. J. Cancer. 64 (1991) 537-542
35. Nagy A.M., Vanbellinghen A.M., Robyn C., and Meuris S. Epitope mapping on intact, heated and reduced molecular variants of human chorionic gonadotrophin. Mol. Cell. Endocrinol. 122 (1996) 51-57
36. Narayan P., Gray J., and Puett D. Yoked complexes of human choriogonadotropin and the lutropin receptor: evidence that monomeric individual subunits are inactive. Mol. Endocrinol. 16 (2002) 2733-2745
37. Nishimura R., Utsunomiya T., Ide K., Kitajima T., Chen H.C., et al. Characterization of human chorionic gonadotropin in urine of patients with trophoblastic diseases by Western blotting using specific antibodies. Jpn. J. Cancer. Res. 78 (1987) 833-839
38. Pierce J.G., and Parsons T.F. Glycoprotein hormones: structure and function. Annu. Rev. Biochem. 50 (1981) 465-495
39. Puett D., Bhowmick N., Fernandez L.M., Huang J., Wu C., et al. hCG-receptor binding and transmembrane signaling. Mol. Cell. Endocrinol. 125 (1996) 55-64
40. Roche P.C., and Ryan R.J. Purification, characterization, and amino-terminal sequence of rat ovarian receptor for luteinizing hormone/human choriogonadotropin. J. Biol. Chem. 264 (1989) 4636-4641
41. Roche P.C., Ryan R.J., and McCormick D.J. Identification of hormone-binding regions of the luteinizing hormone/human chorionic gonadotropin receptor using synthetic peptides. Endocrinology 131 (1992) 268-274
42. Rotmensch S., and Cole L.A. False diagnosis and needless therapy of presumed malignant disease in women with false-positive human chorionic gonadotropin concentrations [see comments]. Lancet 355 (2000) 712-715
43. Ruddon R.W., Krzesicki R.F., Beebe J.S., Loesel L., Perini F., et al. Conformational intermediates in the production of the combinable form of the beta-subunit of chorionic gonadotropin. Endocrinology 124 (1989) 862-869
44. Singh V., and Merz W.E. Disulfide bond formation is not required for human chorionic gonadotropin subunit association. Studies with dithiothreitol in JEG-3 cells. J. Biol. Chem. 275 (2000) 11765-11770
45. Srilatha N.S., Selvi P.T., and Muthy S. Epitope mapping from real time kinetic studies-role of cross-linked disulphides and incidential interacting regions in affinity measurements: study with human chorionic gonadotropin and monoclonal antibodies. J. Biosci. 30 (2005) 359-370
46. Udagawa A., Okamoto T., Nomura S., Matsuo K., Suzuki H., et al. Human chorionic gonadotropin beta-core fragment is present in the human placenta. Mol. Cell. Endocrinol. 139 (1998) 171-178
47. Vitt U.A., Hsu S.Y., and Hsueh A.J.W. Evolution and classification of cystine knot-containing hormones and related extracellular signaling molecules. Mol. Endocrinol. 15 (2001) 681-694
48. Weinans M.J.N., Butler S.A., Mantingh A., and Cole L.A. Urinary hyperglycosylated hCG in first trimester screening for chromosomal abnormalities. Prenat. Diagn. 20 (2000) 976-978
49. Wu H., Lustbader J.W., Liu Y., Canfield R.E., and Hendrickson W.A. Structure of human chorionic gonadotropin at 2.6 A resolution from MAD analysis of the selenomethionyl protein. Structure 2 (1994) 545-558
50. Xing Y., Myers R.V., Cao D., Lin W., Jiang M., Bernard M.P., and Moyle W.R. Glycoprotein hormone assembly in the endoplasmic reticulum. I. The glycosylated end of human α-subunit loop 2 is threaded through a β-subunit hole. J. Biol. Chem. 279 (2004) 35426-35436
51. Xing Y., Myers R.V., Cao D., Lin W., Jiang M., Bernard M.P., and Moyle W.R. Glycoprotein hormone assembly in the endoplasmic reticulum. II. Multiple roles of a redox-sensitive β-subunit disulfide switch. J. Biol. Chem. 279 (2004) 35437-35448