Epitope mapping from real time kinetic studies - Role of cross-linked disulphides and incidental interacting regions in affinity measurements: Study with human chorionic gonadotropin and monoclonal antibodies

Journal of Biosciences

Volumn 30, Issue 3, 2005, Pages 359-370

  Srilatha, Nonavinakere Seetharam ^a   Selvi, P Tamil ^a   Murthy, Gundlupet Satyanarayana ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Association constant; Epitope mapping; Incidental interacting regions; Kinetics; Monoclonal antibody

Indexed keywords

CHORIONIC GONADOTROPIN; DISULFIDE; MONOCLONAL ANTIBODY;

ANTIBODY;

ANTIBODY COMBINING SITE; ARTICLE; CONTROLLED STUDY; CROSS LINKING; EPITOPE MAPPING; HUMAN; IONIC STRENGTH; KINETICS; PROTEIN PROTEIN INTERACTION;

EID: 22244439239     PISSN: 02505991     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02703673     Document Type: Article

References (27)

1. Ashish B, Venkatesh N and Murthy G S 2002a Structure function analysis: Lessons from human chorionic gonadotropin; Indian J. Exp. Biol. 40 434-447
2. Ashish B, Srilatha N S and Murthy G S 2002b Real time kinetic analysis of hCG-monoclonal antibody interaction using radiolabelled hCG probe: Presence of two forms of Ag-MAb complex as revealed by solid phase dissociation studies; Biochim. Biophys. Acta 1569 21-30
3. Ashish B, Tamil Selvi P and Murthy G S 2002c Thermodynamics of hCG-monoclonal antibody interaction: an analysis of real time kinetics data obtained using radiolabelled hCG probe; Biochim. Biophys. Acta 1572 31-36
4. Berger P, Bidart J M, Delves P S, Dirnhofer S, Hoermann R, Isaacs N, Jackson A, Klonisch T, Lapthorn A J, Lund T, Mann K, Roitt I, Schwarz S and Wick G 1996 Immunochemical mapping of gonadotropins; Mol. Cell Endocrinol. 123 33-43
5. Fraker P J and Speck J C 1978 Protein and cell membrane iodination with sparingly soluble chloramide 1, 3, 4, 6-tetrachloro-3, 6-diphenylglycouril; Biochem. Biophys. Res. Commun. 80 849-857
6. Jackson A M, Berger P, Pixley M, Klein C, Hsueh A J and Biome I 1999 The biological action of choriogonadotropin is not dependent on the complete native quaternary interactions between the subunits; Mol. Endocrinol. 13 2175-2188
7. Kumar S and Nussinov R 2002 Relationship between ion pair geometries and electrostatic strengths in proteins; Biophy. J. 83 1595-1612
8. Lapthorn A J, Harris D C, Littlejohn A, Lustbader J W, Canfield R E, Machin K J, Morgan F J and Isaacs N W 1994 Crystal Structure of human chorionic gonadotropin; Nature (London) 369 455-461
9. Li Y, Li H, Yang F, Smith-Gill S J and Mariuzza R A 2003 X-ray snap shots of the maturation of the antibody response to a protein antigen; Nat. Struct. Biol. 10 482-488
10. Lobel L, Pollak S, Wang S, Chaney M and Lustbader J W 1999 Expression and Characterization of recombinant β-subunit hCG homodimer; Endocrine 10 261-270
11. Matzuk M M, Hsueh A J W, LaPolt P, Tsafriri A, Keene J L and Biome I 1990 The biological role of the carboxy-terminal extension of human chorionic gonadotropin; Endocrinology 126 376-383
12. Mohan S, Sinha N and Smith-Gill S J 2002 Differences in electrostatic properties at antibody-antigen binding sites: implications for specificity and cross-reactivity; Biophys. J. 83 2946-2968
13. Mohan S, Sinha N and Smith-Gill S J 2003 Modeling the bind-ing sites of anti-hen egg white lysozyme antibodies Hy-HEL-8 and Hy-HEL-26: an insight into the molecular basis of cross-reactivity and specificity; Biophys. J. 85 3221-3236
14. Murthy G S 1996 Real time kinetic analysis of antigen-antibody interaction using solid phase binding: Transformation of hCG- monoclonal antibody complex; Curr. Sci. 71 981-988
15. Murthy G S and Srilatha N S 1996 Mapping of assembled epitopes with microgram quantities of antigen: Identification of an epitope at the receptor binding region of hFSH; Curr. Sci. 70 1019-1022
16. Murthy G S and Venkatesh N 1996 Determination of kinetics of epitope paratope interaction based on solid phase binding: an inexpensive alternative to biospecific interaction analysis; J. Biosci. 21641-651
17. Purohit S, Shao K, Balasubramanian S V and Bahl O P 1997 Mutants of human chorionic gonadotropin lacking N-glycosyl chain in the α-subunit. 1. Mechanism for the differential action of the N-linked carbohydrates; Biochemistry 36 12355-12363
18. Sinha N, Mohan S, Lipschultz C A and Smith-Gill S J 2002 Electrostatics in protein binding and function; Curr. Protein Pept. Sci. 3 601-614
19. Sinha N and Smith Gill S J 2002 Protein structure to function via dynamics; Protein Pept. Lett. 9 367-377
20. Srilatha N S and Murthy G S 1996 Mapping of assembled epitopic regions of human chorionic gonadotropin reveals proximity of CTPα to the determinant loop β93-100; J. Biosci. 21 755-764
21. Srilatha N S and Murthy G S 2002 Study of dissociation of human chorionic gonadotropin monoclonal antibody complexes using nitrocellulose as an insoluble support; Curr. Sci. 78 1548-1552
22. Tamil Selvi P, Ashish B and Murthy G S 2002 Determination of thermodynamic parameters of antigen-antibody interaction from real time kinetic studies; Curr. Sci. 82 1442-1448
23. Troalen F, Bellet B H, Ghillani P, Puisieux A, Bohuon C J and Bidart J M 1988 Antigenic determinants on human chorionic gonadotropin a subunit. II. Immunochemical mapping by a monoclonal anti-peptide antibody; J. Biol. Chem. 263 10370-10376
24. Venkatesh N, Nagaraja G and Murthy G S 1995 Analysis of a conformation specific epitope of the alpha subunit of human chorionic gonadotropin: Study using monoclonal antibody probes; Curr. Sci. 69 48-55
25. Venkatesh N and Murthy G S 1997a Immunochemical approach to the mapping of an assembled epitope of human chorionic gonadotropin: Proximity of CTP-α to the receptor binding region of the B-subunit; J. Immunol. Methods 202 173-182
26. Venkatesh N and Murthy G S 1997b Stability of assembled epitopes of human chorionic gonadotropin to covalent modifications: Analysis using Monoclonal antibody probes; Biochem. Mol. Biol. Int. 42 853-863
27. Venkatesh N, Krishnaswamy S, Meuris S and Murthy G S 1999 Epitope analysis and molecular modelling reveals the topography of the C-terminal peptide of β subunit of hCG; Eur. J. Biochem. 265 1061-1066