Human chorionic gonadotropin β-core fragment is present in the human placenta

Molecular and Cellular Endocrinology

Volumn 139, Issue 1-2, 1998, Pages 171-178

  Udagawa, Atsuko ^a,b   Okamoto, Tomomitsu ^a   Nomura, Seiji ^a,b   Matsuo, Katsuhiko ^a,b   Suzuki, Hideo ^a,b   Mizutani, Shigehiko ^a,b  

^a NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b Tsukuba Research Laboratory   (Japan)

Author keywords

core fragment; hCG; Placenta (human placenta)

Indexed keywords

CHORIONIC GONADOTROPIN BETA SUBUNIT;

ARTICLE; ENZYME IMMUNOASSAY; FEMALE; HORMONE DETERMINATION; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MOLECULAR WEIGHT; ORGAN CULTURE; PLACENTA; PREGNANCY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SUPERNATANT; SYNCYTIOTROPHOBLAST; TROPHOBLAST; URINALYSIS;

CELL EXTRACTS; CELLS, CULTURED; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; ORGAN CULTURE TECHNIQUES; PEPTIDE FRAGMENTS; PLACENTA; PREGNANCY; SENSITIVITY AND SPECIFICITY; TROPHOBLASTS;

EID: 0345367958     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(98)00062-8     Document Type: Article

References (22)

1. Alfthan H., Stenman U.-H. Pregnancy serum contains the β-core fragment of human choriogonadotrophin. J. Clin. Endocrinol. Metab. 70:1990;783-787.
2. Birken S., Armstrong E.G., Kolks M.A.G., Cole L.A., Agosto G.M., Krichevsky A., Vaitukaitis J.L., Canfield R.E. Structure of the human chorionic gonadotropin β-subunit fragment from pregnant urine. Endocrinology. 123:1988;572-583.
3. Blithe D.L., Akar A.H., Wehmann R.E., Nisula B.C. Purification of β-core fragment from pregnancy urine and demonstration that its carbohydrate moieties differ from those of native human chorionic gonadotropin-β Endocrinology. 122:1988;173-180.
4. Canick, J.A., Kellner, L.H., Saller, D.N., Palomaki, G.E., Walker R.P., Osathanondh, R. Second-trimester levels of maternal urinary gonadotropin peptide in Down syndrome pregnancy. Prenat. Diagn. 15, 739-744.
5. Cole L.A., Birken S. Origin and occurrence of human chorionic gonadotropin β-subunit core fragment. Mol. Endocrinol. 2:1988;825-830.
6. Cole L.A., Wang Y., Elliot M., Latif M., Chambers J.T., Chambers S.K., Schwartz P.E. Urinary human chorionic gonadotropin free β-subunit and β-core fragment: A new marker of gynecological cancers. Cancer Res. 48:1988;1356-1360.
7. Cuckle H.S., Ile R.K., Chard T. Urinary β-core human chorionic gonadotrophin: a new approach to Down's syndrome screening. Prenat. Diagn. 14:1994;953-958.
8. De Medeiros S.F., Amato F., Matthews C.D., Norman R.J. Urinary concentrations of beta core fragment of hCG throughout pregnancy. Obstet. Gynecol. 80:1992;223-228.
9. De Medeiros S.F., Amato F., Matthews C.D., Norman R.J. Molecular heterogeneity of the β-core fragment of human chorionic gonadotrophin. J. Endocrinol. 139:1993;519-532.
10. Horibe N., Okamoto T., Itakura A., Nakanishi T., Suzuki T., Kazeto S., Tomoda Y. Levels of hepatocyte growth factor in maternal serum and amniotic fluid. Am. J. Obstet. Gynecol. 173:1995;937-942.
11. Kardana A., Cole L.A. Serum hCG β-core fragment is masked by associated macromolecules. J. Clin. Endocrinol. Metab. 71:1990;1393-1395.
12. Kardana A., Taylor M.E., Southall P.J., Boxer G.M., Rowan A.J., Bagshawe K.D. Urinary gonadotropin peptide-isolation and purification, and its immunohistochemical distribution in normal and neoplastic tissues. Br. J. Cancer. 58:1988;281-286.
13. Kato Y., Braunstein D. β-core fragment is a major form of immunoreactive urinary chorionic gonadotropin in human pregnancy. J. Clin. Endocrinol. Metab. 66:1988;1197-1201.
14. Kinugasa M., Nishimura R., Koizumi T., Morisue K., Higashida T., Natazuka T., Nakagawa T., Isobe T., Baba S., Hasegawa K. Combination assay of urinary β-core fragment of human chorionic gonadotropin with serum tumor markers in gynecologic cancers. Jpn. J. Cancer Res. 86:1995;783-789.
15. Kliman H.J., Nestler J.E., Sermasi E., Sanger J.M., Strauss III J.F. Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology. 118:1986;1567-1582.
16. Krichevsky A., Armstrong E.G., Schlatterer J., Birken S., O'Connor J., Bikel K., Silverberg S., Lustbader J.W., Canfield R.E. Preparation and characterization of antibodies to the urinary fragment of the human chorionic gonadotropin β-subunit. Endocrinology. 123:1988;584-593.
17. Krichevsky A., Birken S., O'Connor J., Bikel K., Schlatterer J., Chen Y.I., Agosto G., Canfield R. Development and characterization of a new, highly specific antibody to the human chorionic gonadotropin-β fragment. Endocrinology. 128:1991;1255-1264.
18. Nishimura R., Kitajima T., Hasegawa K., Takeuchi K., Mochizuki M. Molecular forms of human chorionic gonadotropin in choriocarcinoma serum and urine. Jpn. J. Cancer Res. 80:1989;968-974.
19. Papapetrou P.D., Nicopoulou S.C. The origin of a human chorionic gonadotropin beta-subunit-core fragment excreted in the urine of patients with cancer. Acta Endocrinol. 112:1986;415-422.
20. Towbin H.T., Stachlin T., Fordin J.H. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4357.
21. Wehmann R.E., Blithe D.L., Flack M.R., Nisula B.C. Metabolic clearance rate and urinary clearance of purified β-core. J. Clin. Endocrinol. Metab. 69:1989;510-517.
22. Wehmann R.E., Blithe D.L., Akar A.H., Nisula B.C. Disparity between β-core levels in pregnancy urine and serum: implication for the origin of urinary β-core. J. Clin. Endocrinol. Metab. 70:1990;371-378.