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Volumn 5, Issue 11, 2006, Pages 2977-2984

Toward plasma proteome profiling with ion mobility-mass spectrometry

Author keywords

Ion mobility spectrometry; Multidimensional separations; Proteome profiling

Indexed keywords

PROTEOME;

EID: 33750988179     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr060232i     Document Type: Article
Times cited : (129)

References (96)
  • 34
    • 33750999998 scopus 로고    scopus 로고
    • note
    • For reports of recent results for human plasma proteomics studies using different analytical methods see the 2005 issue 13 of the journal Proteomics. The entire issue contains reports of results from HUPO's Plasma Proteome Initiative.
  • 68
    • 33751002120 scopus 로고    scopus 로고
    • note
    • All research was carried out using an approved protocol by the Indiana University Human Subjects Committee (#04-9243).
  • 72
    • 33750999137 scopus 로고    scopus 로고
    • Available at ftp://us.expasy.org/databases/swiss-prot
    • Available at ftp://us.expasy.org/databases/swiss-prot/.
  • 78
    • 33751013803 scopus 로고    scopus 로고
    • note
    • We note that the selection of ±0.1 Da. is not limited by the resolving power or mass accuracy (∼4500 and 20-50 ppm, respectively) of the home-built TOF instrument. The wider selection has been used to allow for sampling of very low-abundance fragment ions (i.e., ions in such low abundance that "statistically significant" TOF peak distributions are not obtained). Again, the mobility dispersion allows the classification of clusters of low-intensity bins as signal because they are removed from interfering chemical noise. Overall, from careful examination of random false positive assignments as well as classical plasma protein assignments, the wider range does not significantly increase false positive rates.
  • 79
    • 33750985743 scopus 로고    scopus 로고
    • note
    • The reverse protein database was generated by inverting the protein sequences in the Swiss-Prot protein database.
  • 85
    • 33750979082 scopus 로고    scopus 로고
    • Thermo Electron Corporation Application Notes
    • Thermo Electron Corporation Application Notes http://www.thermo.com/ eThermo/CMA/PDFs/Articles/articles-File_21825.pdf.
  • 86
    • 33750977030 scopus 로고    scopus 로고
    • Thermo Electron Corporation Application Notes
    • Thermo Electron Corporation Application Notes http://www.thermo.com/ eThermo/CMA/PDFs/Articles/articles-File_23993.pdf.
  • 88
    • 33751020630 scopus 로고    scopus 로고
    • note
    • Here we do not assess the coefficient of variation (CV) in the intensities of these identified features because the LC injection was performed manually and is likely to result in significant variation. Additionally, the home-built TOF detector has a tendency to saturate at relatively low peptide concentrations because of the ion "bunching" caused by the mobility separation. Current instrumental efforts involve the coupling of an LC system with an autosampler as well as the installation of a commercial TOF detector (and the development of an analogue detection scheme). Although the successful implementation of these improvements will enhance the ability to determine accurate CVs for peptide ion intensity, we note that the reproducibility studies described in the text are aimed at determining the ability to align two different datasets (necessary for comparative mapping studies).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.