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We note that the selection of ±0.1 Da. is not limited by the resolving power or mass accuracy (∼4500 and 20-50 ppm, respectively) of the home-built TOF instrument. The wider selection has been used to allow for sampling of very low-abundance fragment ions (i.e., ions in such low abundance that "statistically significant" TOF peak distributions are not obtained). Again, the mobility dispersion allows the classification of clusters of low-intensity bins as signal because they are removed from interfering chemical noise. Overall, from careful examination of random false positive assignments as well as classical plasma protein assignments, the wider range does not significantly increase false positive rates.
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