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Volumn 414, Issue , 2006, Pages 364-389

Generation and Characterization of a Stable MK2-EGFP Cell Line and Subsequent Development of a High-Content Imaging Assay on the Cellomics ArrayScan Platform to Screen for p38 Mitogen-Activated Protein Kinase Inhibitors

Author keywords

[No Author keywords available]

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; 5 (2,6 DICHLOROPHENYL) 2 (2,4 DIFLUOROPHENYLTHIO)PYRIMIDO[1,6 B]PYRIDAZIN 6 ONE; 6 AMINO 2 (4 FLUOROPHENYL) 4 METHOXY 3 (4 PYRIDYL) 1H PYRROLO[2,3 B]PYRIDINE; ANISOMYCIN; DIMETHYL SULFOXIDE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE P38; MITOGEN ACTIVATED PROTEIN KINASE P38 INHIBITOR; SB 242235; STAUROSPORINE; STRESS ACTIVATED PROTEIN KINASE; UNCLASSIFIED DRUG; UO 126; WORTMANNIN;

EID: 33750813639     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(06)14021-5     Document Type: Chapter
Times cited : (33)

References (24)
  • 2
    • 0037389558 scopus 로고    scopus 로고
    • Mitogen-activated protein kinases: New signaling pathways functioning in cellular responses to environmental stress
    • Cowan K.J., and Storey K.B. Mitogen-activated protein kinases: New signaling pathways functioning in cellular responses to environmental stress. J. Exp. Biol. 206 (2003) 1107-1115
    • (2003) J. Exp. Biol. , vol.206 , pp. 1107-1115
    • Cowan, K.J.1    Storey, K.B.2
  • 3
    • 0023433311 scopus 로고
    • Five-parameter fluorescence imaging: Wound healing of living Swiss 3T3 cells
    • DeBiasio R., Bright G.R., Ernst L.A., Waggoner A.S., and Taylor D.L. Five-parameter fluorescence imaging: Wound healing of living Swiss 3T3 cells. J. Cell Biol. 105 (1987) 1613-1622
    • (1987) J. Cell Biol. , vol.105 , pp. 1613-1622
    • DeBiasio, R.1    Bright, G.R.2    Ernst, L.A.3    Waggoner, A.S.4    Taylor, D.L.5
  • 4
    • 0032526988 scopus 로고    scopus 로고
    • Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation
    • Engel K., Kotlyarov A., and Gaestel M. Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation. EMBO J. 17 (1998) 3363-3371
    • (1998) EMBO J. , vol.17 , pp. 3363-3371
    • Engel, K.1    Kotlyarov, A.2    Gaestel, M.3
  • 5
    • 0036165251 scopus 로고    scopus 로고
    • Pharmacological inhibitors of MAPK pathways
    • English J.M., and Cobb M.H. Pharmacological inhibitors of MAPK pathways. Trends Pharmacol. Sci. 23 (2002) 40-45
    • (2002) Trends Pharmacol. Sci. , vol.23 , pp. 40-45
    • English, J.M.1    Cobb, M.H.2
  • 7
    • 0032910168 scopus 로고    scopus 로고
    • Organization and regulation of mitogen activated protein kinase signaling pathways
    • Garrington T.P., and Johnson G.L. Organization and regulation of mitogen activated protein kinase signaling pathways. Curr. Opin. Cell Biol. 11 (1999) 211-218
    • (1999) Curr. Opin. Cell Biol. , vol.11 , pp. 211-218
    • Garrington, T.P.1    Johnson, G.L.2
  • 9
    • 0032031530 scopus 로고    scopus 로고
    • Fluorescent-protein biosensors: New tools in drug discovery
    • Giuliano K.A., and Taylor D.L. Fluorescent-protein biosensors: New tools in drug discovery. TIBTech. 16 (1998) 135-140
    • (1998) TIBTech. , vol.16 , pp. 135-140
    • Giuliano, K.A.1    Taylor, D.L.2
  • 10
    • 0037085583 scopus 로고    scopus 로고
    • Cellular platforms for HTS: Three case studies
    • Johnston P.A., and Johnston P.A. Cellular platforms for HTS: Three case studies. Drug Discov. Today 7 (2002) 353-363
    • (2002) Drug Discov. Today , vol.7 , pp. 353-363
    • Johnston, P.A.1    Johnston, P.A.2
  • 13
    • 12344250982 scopus 로고    scopus 로고
    • Small-molecule screening and profiling by using automated microscopy
    • Mitchison T.J. Small-molecule screening and profiling by using automated microscopy. Chembiochem. 6 (2005) 33-39
    • (2005) Chembiochem. , vol.6 , pp. 33-39
    • Mitchison, T.J.1
  • 14
    • 0036479125 scopus 로고    scopus 로고
    • MK2 targets AU-rich elements and regulates biosynthesis of tumor necrosis factor and interleukin-6 independently at different post-transcriptional levels
    • Neininger A., Kontoyiannis D., Kotlyarov A., Winzen R., Eckert R., Volk H.D., Holtmann H., Kollias G., and Gaestel M. MK2 targets AU-rich elements and regulates biosynthesis of tumor necrosis factor and interleukin-6 independently at different post-transcriptional levels. J. Biol. Chem. 277 (2002) 3065-3068
    • (2002) J. Biol. Chem. , vol.277 , pp. 3065-3068
    • Neininger, A.1    Kontoyiannis, D.2    Kotlyarov, A.3    Winzen, R.4    Eckert, R.5    Volk, H.D.6    Holtmann, H.7    Kollias, G.8    Gaestel, M.9
  • 15
    • 0034859534 scopus 로고    scopus 로고
    • FRET-based detection of different conformations of MK2
    • Neininger A., Thielemann H., and Gaestel M. FRET-based detection of different conformations of MK2. EMBO Rep. 2 (2001) 703-708
    • (2001) EMBO Rep. , vol.2 , pp. 703-708
    • Neininger, A.1    Thielemann, H.2    Gaestel, M.3
  • 16
    • 1642323740 scopus 로고    scopus 로고
    • Protein kinase inhibitors: Insights into drug design and structure
    • Noble M.E.M., Endicott J.A., and Johnson L.N. Protein kinase inhibitors: Insights into drug design and structure. Science 303 (2004) 1800-1805
    • (2004) Science , vol.303 , pp. 1800-1805
    • Noble, M.E.M.1    Endicott, J.A.2    Johnson, L.N.3
  • 17
    • 0033965158 scopus 로고    scopus 로고
    • The p38 signal transduction pathway, activation and function
    • Ono K., and Han J. The p38 signal transduction pathway, activation and function. Cell. Signal. 12 (2000) 1-13
    • (2000) Cell. Signal. , vol.12 , pp. 1-13
    • Ono, K.1    Han, J.2
  • 20
    • 0034883501 scopus 로고    scopus 로고
    • Using green fluorescent protein to study intracellular signaling
    • Tavare J.M., Fletcher L.M., and Welsh G.I. Using green fluorescent protein to study intracellular signaling. J. Endocrinol. 170 (2001) 297-306
    • (2001) J. Endocrinol. , vol.170 , pp. 297-306
    • Tavare, J.M.1    Fletcher, L.M.2    Welsh, G.I.3
  • 21
    • 15744405770 scopus 로고    scopus 로고
    • Stealth sensors: Real-time monitoring of the cell cycle
    • Thomas N., and Goodyer D. Stealth sensors: Real-time monitoring of the cell cycle. Targets 2 (2003) 26-33
    • (2003) Targets , vol.2 , pp. 26-33
    • Thomas, N.1    Goodyer, D.2
  • 22
    • 33750815755 scopus 로고    scopus 로고
    • Assay development and case history of a 32K-biased library high-content MK2-EGFP translocation screen to identify p38 MAPK inhibitors on the ArrayScan 3.1 imaging platform
    • (this volume)
    • Trask Jr. O.J., Baker A., Williams R.G., Kickischer D., Kandasamy R., Laethem C., Johnston P.A., and Johnston P.A. Assay development and case history of a 32K-biased library high-content MK2-EGFP translocation screen to identify p38 MAPK inhibitors on the ArrayScan 3.1 imaging platform. Methods Enzymol. 414 (2006) (this volume)
    • (2006) Methods Enzymol. , vol.414
    • Trask Jr., O.J.1    Baker, A.2    Williams, R.G.3    Kickischer, D.4    Kandasamy, R.5    Laethem, C.6    Johnston, P.A.7    Johnston, P.A.8
  • 23
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • Zhang J.H., Chung T.D., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73
    • (1999) J. Biomol. Screen. , vol.4 , pp. 67-73
    • Zhang, J.H.1    Chung, T.D.2    Oldenburg, K.R.3
  • 24
    • 0028883299 scopus 로고
    • Characterization of an autoinhibitory domain in human mitogen-activated protein kinase-activated protein kinase 2
    • Zu Y.L., Ai Y., and Huang C.K. Characterization of an autoinhibitory domain in human mitogen-activated protein kinase-activated protein kinase 2. J. Biol. Chem. 270 (1995) 202-206
    • (1995) J. Biol. Chem. , vol.270 , pp. 202-206
    • Zu, Y.L.1    Ai, Y.2    Huang, C.K.3


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