A novel system of genetic transformation allows multiple integrations of a desired gene in Saccharomyces cerevisiae chromosomes

Journal of Microbiological Methods

Volumn 67, Issue 3, 2006, Pages 437-445

  Guerra, Odanir Garcia ^a,b   Rubio, Ileana G S ^a   da Silva Filho, Claudionor Gomes ^a   Bertoni, Regiane Aparecida ^a   dos Santos Govea, Rute Cardoso ^a   Vicente, Elisabete José ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF MATO GROSSO DO SUL   (Brazil)

Author keywords

integration; Glucoamylase; Retrotransposon Ty1; S. cerevisiae transformation; Yeast linear transformation vector; Yeast transformation vector without selective marker

Indexed keywords

ANTIBIOTIC G 418; COMPLEMENTARY DNA; GLUCAN 1,4 ALPHA GLUCOSIDASE;

ANTIBIOTIC RESISTANCE; ARTICLE; ASPERGILLUS AWAMORI; AUXOTROPHIC MUTANT; CHROMOSOME; CONTROLLED STUDY; DNA FLANKING REGION; FREQUENCY ANALYSIS; FUNGAL GENETICS; FUNGAL STRAIN; GENE EXPRESSION; GENE MUTATION; GENE SEQUENCE; GENE VECTOR; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENOME; LABORATORY; MARKER GENE; MOLECULAR DYNAMICS; MOLECULAR STABILITY; NONHUMAN; PLASMID; PRESSURE; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; WILD TYPE; YEAST CELL;

ASPERGILLUS; CHROMOSOMES, FUNGAL; DNA, COMPLEMENTARY; DNA, FUNGAL; FUNGAL PROTEINS; GENETIC ENGINEERING; GENETIC VECTORS; GLUCAN 1,4-ALPHA-GLUCOSIDASE; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SELECTION (GENETICS); TRANSFORMATION, GENETIC;

ASPERGILLUS AWAMORI; SACCHAROMYCES CEREVISIAE;

EID: 33750421187     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.04.014     Document Type: Article

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