Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors

Journal of Virological Methods

Volumn 138, Issue 1-2, 2006, Pages 85-98

  Reed, Sharon E ^a   Staley, Elizabeth M ^b   Mayginnes, John P ^a   Pintel, David J ^a   Tullis, Gregory E ^c  

^a University of Missouri   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^c BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adeno associated virus; DNA transfection; Gene therapy; Polyethylenimine; Transduction assay

Indexed keywords

CALCIUM PHOSPHATE; E1A PROTEIN; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; PARVOVIRUS VECTOR; POLYETHYLENEIMINE; PROTEIN VP1; PROTEIN VP2; PROTEIN VP3; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR E2A; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS PROTEIN E2A; VIRUS PROTEIN E4ORF6; VIRUS RNA;

ARTICLE; CELL LINE; CELL STRAIN HEK293; CONTROLLED STUDY; DNA TRANSFECTION; FLOW CYTOMETRY; GENE EXPRESSION SYSTEM; GENETIC TRANSDUCTION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; VIRUS CAPSID; VIRUS RECOMBINANT; VIRUS RECOMBINATION; VIRUS REPLICATION;

CELL LINE; DEPENDOVIRUS; DNA, VIRAL; FLOW CYTOMETRY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; POLYETHYLENEIMINE; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; TRANSFECTION;

ADENO-ASSOCIATED VIRUS; ADENOVIRIDAE; MAMMALIA; MIRIDAE;

EID: 33750295885     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.07.024     Document Type: Article

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