Oxidized ATP decreases β-actin expression and intracellular superoxide concentrations in RBA-2 type-2 astrocytes independently of P2X7 receptor

European Journal of Pharmacology

Volumn 550, Issue 1-3, 2006, Pages 1-7

  Chen, Hammer B ^a   Lin, Ya Hui ^a   Sun, Synthia H ^a  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

actin; Astrocytes; Oxidized ATP; P2X7 receptor; RBA 2 astrocytes

Indexed keywords

3' O (4 BENZOYLBENZOYL)ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; BETA ACTIN; BRILLIANT BLUE G; INTERLEUKIN 1BETA CONVERTING ENZYME; OXIDIZED ADENOSINE TRIPHOSPHATE; PURINE P2X7 RECEPTOR; PURINERGIC RECEPTOR BLOCKING AGENT; PYRIDOXAL PHOSPHATE 6 AZOPHENYL 2',4' DISULFONIC ACID; SUPEROXIDE; SURAMIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ASTROCYTE; CELL VIABILITY; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ACTINS; ADENOSINE TRIPHOSPHATE; ASTROCYTES; BLOTTING, WESTERN; CASPASE 1; CELL LINE; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; INTRACELLULAR SPACE; OXIDATION-REDUCTION; RECEPTORS, PURINERGIC P2; RECEPTORS, PURINERGIC P2X7; SUPEROXIDES; TETRAZOLIUM SALTS; THIAZOLES;

EID: 33749985038     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2006.08.039     Document Type: Article

References (26)

1. 7 receptors. J. Biol. Chem. 278 (2003) 37344-37351
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
3. Beigi R.D., Kertesy S.B., Aquilina G., and Dubyak G.R. Oxidized ATP (oxidized ATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms. Br. J. Pharmacol. 140 (2003) 507-519
4. Chen H.B., Chan Y.T., Hung A.C., Tsai Y.C., and Sun S.H. Elucidation of ATP-stimulated stress protein expression of RBA-2 type-2 astrocytes: ATP potentiate HSP60 and Cu/Zn SOD expression and stimulates pI shift of peroxiredoxin II. J. Cell. Biochem. 97 (2006) 314-326
5. Crawford L.E., Milliken E.E., Irani K., Zweier J.L., Becker L.C., Johnson T.M., Eissa N.T., Crystal R.G., Finkel T., and Goldschmidt-Clermont P.J. Superoxide-mediated actin response in post-hypoxic endothelial cells. J. Biol. Chem. 271 (1996) 26863-26867
6. Dell'Antonio G., Quattrini A., Dal C.E., Fulgenzi A., and Ferrero M.E. Antinociceptive effect of a new P(2Z)/P2X7 antagonist, oxidized ATP, in arthritic rats. Neurosci. Lett. 327 (2002) 87-90
7. Dell'Antonio G., Quattrini A., Cin E.D., Fulgenzi A., and Ferrero M.E. Relief of inflammatory pain in rats by local use of the selective P2X7 ATP receptor inhibitor, oxidized ATP. Arthritis Rheum. 46 (2002) 3378-3385
8. Fulge A., Dell'Antonio G., Foglieni C., Dal Cin E., Ticozzi P., Franzone J.S., and Ferrero M.E. Inhibition of chemokine expression in rat inflamed paws by systemic use of the antihyperalgesic oxidized ATP. BMC Immunol. 6 18 (2005) 1-11
9. Guenal I., Risler Y., and Mignotte B. Down-regulation of actin genes precedes microfilament network disruption and actin cleavage during p53-mediated apoptosis. J. Cell Sci. 110 (1997) 489-495
10. Hung A.C., Chu Y.J., Lin Y.H., Weng J.Y., Chen H.B., Au Y.C., and Sun S.H. Roles of protein kinase C in regulation of P2X7 receptor-mediated calcium signalling of cultured type-2 astrocyte cell line, RBA-2. Cell. Signal. 17 (2005) 1384-1396
11. Jiang L.H., Mackenzie A.B., North R.A., and Surprenant A. Brilliant blue G selectively blocks ATP-gated rat P2X7 receptors. Mol. Pharmacol. 58 (2000) 82-88
12. A receptors of a rat brain-derived type-1 astrocyte cell line, IA-1g1. Cell. Signal. 15 (2003) 197-207
13. Kim M., Jiang L.H., Wilson H.L., North R.A., and Surprenant A. Proteomic and functional evidence for a P2X7 receptor signalling complex. EMBO J. 20 (2001) 6347-6358
14. Kim M., Spelta V., Sim J., North R.A., and Surprenant A. Differential assembly of rat purinergic P2X7 receptor in immune cells of the brain and periphery. J. Biol. Chem. 276 (2001) 23262-23267
15. Kumar S. ICE-like proteases in apoptosis. Trends Biochem. Sci. 20 (1995) 198-202
16. Li Q., Luo X., Zeng W., and Muallem S. Cell-specific behavior of P2X7 receptors in mouse parotid acinar and duct cells. J. Biol. Chem. 278 (2003) 47554-47561
17. Martinou J.C., and Sadoul R. ICE-like proteases execute the neuronal death program. Curr. Opin. Neurobiol. 6 (1996) 609-614
18. Mashima T., Naito M., Fujita N., Noguchi K., and Tsuruo T. Identification of actin as a substrate of ICE and an ICE-like protease and involvement of an ICE-like protease but not ICE in VP-16-induced U937 apoptosis. Biochem. Biophys. Res. Commun. 217 (1995) 1185-1192
19. Murgia M., Hanau S., Pizzo P., Rippa M., and Di V.F. Oxidized ATP, an irreversible inhibitor of the macrophage purinergic P2Z receptor. J. Biol. Chem. 268 (1993) 8199-8203
20. Parvathenani L.K., Tertyshnikova S., Greco C.R., Roberts S.B., Robertson B., and Posmantur R. P2X7 mediates superoxide production in primary microglia and is up-regulated in a transgenic mouse model of Alzheimer's disease. J. Biol. Chem. 278 (2003) 13309-13317
21. Pfeiffer Z.A., Aga M., Prabhu U., Watters J.J., Hall D.J., and Bertics P.J. The nucleotide receptor P2X7 mediates actin reorganization and membrane blebbing in RAW 264.7 macrophages via p38 MAP kinase and Rho. J. Leukoc. Biol. 75 (2004) 1173-1182
22. Suh B.C., Kim J.S., Namgung U., Ha H., and Kim K.T. P2X7 nucleotide receptor mediation of membrane pore formation and superoxide generation in human promyelocytes and neutrophils. J. Immunol. 166 (2001) 6754-6763
23. 2+ influx and phospholipase D activities of a rat brain-derived type-2 astrocyte cell line, RBA-2, are mediated through P2X7 receptors. J. Neurochem. 73 (1999) 334-343
24. - dependent mechanism. Glia 37 (2002) 8-18
25. 7 purinoceptor-stimulated TGF-beta 1 mRNA expression involves PKC/MAPK signalling pathway in a rat brain-derived type-2 astrocyte cell line, RBA-2. Cell. Signal. 15 (2003) 1129-1137
26. Wang X., Arcuino G., Takano T., Lin J., Peng W.G., Wan P., Li P., Xu Q., Liu Q.S., Goldman S.A., and Nedergaard M. P2X7 receptor inhibition improves recovery after spinal cord injury. Nat. Med. 10 (2004) 821-827