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Inhibition of the Caenorhabditis elegans cell-death protease CED-3 by a CED-3 cleavage site in baculovirus p35 protein
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of special interest. The baculovirus protein p35 blocks four ICE-like proteases and inhibits death in C. elegans. CrmA has death-blocking activity in the same organism only when the CED-3 cleavage site of p35 is introduced into the protein. It is suggested that p35 blocks cell death by acting as a competitive inhibitor of ICE-like proteases.
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of special interest Xue D, Horvitz HR. Inhibition of the Caenorhabditis elegans cell-death protease CED-3 by a CED-3 cleavage site in baculovirus p35 protein. Nature. 377:1995;248-251 The baculovirus protein p35 blocks four ICE-like proteases and inhibits death in C. elegans. CrmA has death-blocking activity in the same organism only when the CED-3 cleavage site of p35 is introduced into the protein. It is suggested that p35 blocks cell death by acting as a competitive inhibitor of ICE-like proteases.
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of special interest. This paper demonstrates that p35 forms stable complexes with ICE and thereby almost irreversibly inhibits the protease.
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of special interest Bump NJ, Hackett M, Hugunin M, Seshagiri S, Brady K, Chen P, Ferenz C, Franklin S, Ghayur T, Li P, et al. Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35. Science. 269:1995;1885-1888 This paper demonstrates that p35 forms stable complexes with ICE and thereby almost irreversibly inhibits the protease.
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Bump, N.J.1
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Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
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of special interest Chinnaiyan AM, Orth K, O'Rourke K, Duan HJ, Poirier GG, Dixit VM. Molecular ordering of the cell death pathway - Bcl-2 and Bcl-X(L) function upstream of the Ced-3-like apoptotic proteases. J Biol Chem. 271:1996;4573-4576 Overexpression of Bcl-2 and Bcl-x prevents the cleavage of CPP32 and ICE-LAP3 cysteine proteases that occurs in Fas- and staurosporine-induced apoptosis of Jurkat cells. These results demonstrate that Bcl-2 and Bcl-x function upstream of CPP32 and ICE-LAP3.
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Bcl-2 prevents activation of CPP32 cysteine protease and cleavage of poly(ADP-ribose) polymerase and U1-70kD proteins in staurosporine-mediated apoptosis
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0028984948
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