The human homologue of the yeast splicing factor Prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions

Journal of Molecular Biology

Volumn 298, Issue 4, 2000, Pages 567-575

  Makarov, Evgeny M ^a   Makarova, Olga V ^a   Achsel, Tilmann ^a   Lührmann, Reinhard ^a,b  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Pre mRNA splicing; Prp; TPR; U5 102kD protein; U4 U6.U5 tri snRNP

Indexed keywords

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RNA SPLICING; SEQUENCE ANALYSIS; SPLICEOSOME; YEAST;

EID: 0034640124     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3685     Document Type: Article

References (30)

1. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding
2. The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2 and a novel WD-40 protein
3. A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
4. Functional and structural characterization of the prp3 binding domain of the yeast prp4 splicing factor
5. Immunoaffinity purification of a [U4/U6. U5] tri-snRNP from human cells
6. Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6. U5 snRNP addition
7. The structure of the tetratricopeptide repeats of protein phosphatase 5: Implications for TPR-mediated protein-protein interactions
8. Protein-RNA interactions in the U5 snRNP of Saccharomyces cerevisiae
9. An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2
10. The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP
11. Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6. U5] tri-snRNP
12. snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions
13. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs
14. The structure and function of proteins involved in mammalian pre-mRNA splicing
15. Tetratrico-peptide-repeat proteins in the archaeon Methanococcus jannaschii
16. Identification of an RNA-dependent ATPase activity in mammalian U5 snRNPs
17. The human U5-200kD DEXH-box protein unwinds U4/ U6 RNA duplices in vitro
18. Tetra-repeat interactions: To TPR or not to TPR?
19. The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p
20. The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors
21. Commitment of yeast pre-mRNA to the splicing pathway requires a novel U1 small nuclear ribonucleoprotein polypeptide, Prp39p
22. Functional interaction of a novel 15. 5kD [U4/U6.U5] tri-snRNP protein with the 5'-stem loop of U4 snRNA
23. A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis
24. Purification of the yeast U4/U6. U5 small nuclear ribonucleoprotein particle and identification of its proteins
25. The 20kD protein of human [U4/U6. U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60kD and 90kD proteins
26. The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p
27. The prpl+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast
28. Characterization of U6 snRNA-protein interactions
29. Protein functions in pre-mRNA splicing