Identification of the catalytic residues of the first family of β(1-3)glucanosyltransferases identified in fungi

Biochemical Journal

Volumn 347, Issue 3, 2000, Pages 741-747

  Mouyna, Isabelle ^a   Monod, Michel ^b   Fontaine, Thierry ^a   Henrissat, Bernard ^c   Léchenne, Barbara ^b   Latgé, Jean Paul ^a  

^a INSTITUT PASTEUR   (France)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c CNRS   (France)

Author keywords

Aspergillus fumigatus; GEL protein family; GPI anchored protein

Indexed keywords

FUNGAL ENZYME; GLUTAMIC ACID; GLYCOSYLTRANSFERASE;

ARTICLE; ASPERGILLUS FUMIGATUS; CLUSTER ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ASPERGILLUS FUMIGATUS; BINDING SITES; BLOTTING, WESTERN; CATALYTIC DOMAIN; CONSERVED SEQUENCE; FUNGAL PROTEINS; GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE; GLUTAMIC ACID; GLYCOSYLPHOSPHATIDYLINOSITOLS; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; MUTATION; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT;

EID: 0034192345     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3470741     Document Type: Article

References (28)

1. 1 Latgé, J. P. (1999) Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12, 310-350
2. 2 Klis, F. M. (1994) Cell wall assembly in yeast. Yeast 10, 851-869
3. 3 Hearn, V. M. and Sietsma, J. H. (1994) Chemical and immunological analysis of the A. lumigatus cell wall. Microbiology 140, 789-795
4. 4 Latgé, J. P., Debeaupuis, J. P., Moutaouakil, M., Diaquin, M., Sarfati, J., Prevost. M. C. Wieruszeski, J. M., Leroy, Y. and Fournet, B. (1991) Galactomannan and the circulating antigens of Aspergillus lumigatus. In Fungal Cell Wall and Immune Response, vol. 53 (Latgé, J. P. and Boucias, D., eds.), pp. 143-151, NATO ASI series, Springer, Berlin and New York
5. 5 Hartland, R. P., Fontaine, T., Debeaupuis, J. P., Simenel, C., Delepierre, M. and Latgé, J. P. (1996) A novel β(1-3)glucanosyltransferase from the cell wall of Aspergillus fumigatus. J. Biol. Chem. 271, 26843-26849
6. 6 Mouyna, I., Fontaine, T., Vai, M., Monod, M., Fonzi, W. A., Diaquin, M., Popolo, L., Hartland, R. P. and Latgé, J. P. (2000) GPI-anchored glucanosyltransferase play an active role in the biosynthesis of the fungal cell wall. J. Biol. Chem., in the press
7. 7 Vai, M., Gatti, E., Lacana, E., Popolo, L. and Alberghina, L. (1991) Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophophoslipid-anchored protein containing a serine-rich region. J. Biol. Chem. 266, 12242-12248
8. 8 Popolo, L., Vai, M., Gatti, E., Porello, S., Bonfante, P., Balestrini, R. and Alberghina, L. (1993) Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation. J. Bacteriol. 175, 1879-1885
9. 9 Saporito-Irwin, S. M., Birse, C. E., Sypherd, P. S. and Fonzi, W. A. (1995) PHR1, a pH regulated gene of Candida albicans is required for morphogenesis. Mol. Cell. Biol. 15, 601-613
10. 10 Muhlschlegel, F. A. and Fonzi, W A. (1997) PHR2 of Candida albicans encodes a functional homolog of the pH-regulated PHR1 with an inverted pattern of pH dependent expression. Mol. Cell. Biol. 17, 5960-5967
11. 11 Nakazawa, T., Horiuchi, H., Ohta, A. and Takagi, M. (1998) Isolation and characterization of EPD1, an essential gene for pseudohyphal growth of a dimorphic yeast, Candida maltosa. J. Bacteriol. 180, 2079-2086
12. 12 Gaboriaud, C., Bissery, V., Benchetrit, T. and Mornon, J. P. (1987) Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences. FEBS Lett. 224, 149-155
13. 13 Henrissat, B. and Bairoch, A. (1993) New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J 293, 781-788
14. 14 Henrissat, B. and Davies, G. (1997) Structural and sequence-based classification of glycoside hydrolases. Curr. Opin. Struct. Biol. 7, 637-644
15. 15 Woodcock, S., Mornon, J. P. and Henrissat, B. (1992) Detection of secondary structure elements in proteins by hydrophobic analysis. Protein Eng. 5, 629-635
16. 16 Lemesle-Varlot, L., Henrissat, B., Gaboriaud, C., Bissery, V., Morgat, A. and Mornon, J. P. (1990) Hydrophobic cluster analysis: procedures to derive structural and functional information from 2D representation of protein sequences. Biochimie 72, 555-574
17. 17 Callebaut, I., Labesse, G., Durand, P., Poupon, A., Canard, L., Chomelier, J., Henrissat, B. and Mornon, J. P. (1997) Deciphering protein sequence information through hydrophobic cluster analysis (HCA): current status and perspectives. Cell. Mol. Life Sci. 53, 621-645
18. 18 Reference deleted
19. 19 Mouyna, I., Hartland, R. P., Fontaine, T., Diaquin, M., Simenel, C., Delepierre, M., Henrissat, B. and Latgé, J. P. (1998) A β(1-3)glucanosyltransferase isolated from the cell wall of Aspergillus is an homolog of the yeast Bgl2p. Microbiology 144, 3171-3180
20. 20 Borg-von Zepelin, M., Beggah, S., Boggian, K., Sanglard, D. and Monod, M. (1998) The expression of the secreted aspartyl proteinases Sap4 and Sap6 from Candida albicans in murine macrophages. Mol. Microbiol. 28, 543-554
21. 21 Goldman, R. C., Sullivan, P. A., Zakula, D. and Capobianco, J. O. (1995) Kinetics of β(1-3)glucan interaction at the donor and acceptor sites of the fungal glucosyltransferase encoded by the BGL2 gene. Eur. J. Biochem. 227, 372-378
22. 22 Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354
23. 23 Popolo, L. and Vai, M. (1999) The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim. Biophys. Acta 1426, 385-400
24. 24 Henrissat, B., Callebaut, I., Fabrega, S., Lehn, P., Mornon, J. P. and Davies, G. (1995) Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92, 7090-7094
25. 25 Jenkins, J., Leggio, L. L., Harris, G. and Pickersgill, R. (1995) β-Glucosidase, β-galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes with 8-fold β/α architecture and with two conserved glutamates near the carboxy-terminal ends of β-strands four and seven. FEBS Lett. 362, 281-285
26. 26 McCarter, J. D. and Withers, S. G. (1994) Mechanisms of enzymic glycoside hydrolysis. Curr. Opin. Struct. Biol. 4, 885-892
27. 27 MacLeod, A. M., Tull, D., Rupitz, K., Warren, R. A. J. and Withers, S. G. (1996) Mechanistic consequences of mutation of active site carboxylases in a retaining β(1-4)glycanase from Cellulomonas fimi. Biochemistry 35, 13165-13172
28. 28 Ducros, V., Czjzek, M., Belaich, A., Gaudin, C., Fierobe, H. P., Belaich, J. P., Davies, G. J. and Haser, R. (1995) Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5. Structure 3, 939-949