Doubt and certainty in counting

Progress in Brain Research

Volumn 135, Issue , 2002, Pages 25-42

  Guillery, R W ^a   August, B K ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; ANALYTIC METHOD; CONFERENCE PAPER; ELECTRON MICROSCOPY; EVALUATION; MEASUREMENT; METHODOLOGY; MICROSCOPY; PRIORITY JOURNAL; RELIABILITY; THICKNESS; VALIDATION PROCESS;

EID: 0036456451     PISSN: 00796123     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0079-6123(02)35005-2     Document Type: Conference Paper

References (43)

1. Abercrombie, M. (1946) Estimation of nuclear population from microtome sections. Anat. Rec., 94: 239-247.
2. Anker, R.L. and Cragg, B.G. (1974) Estimation of the number of synapses in a volume of nervous tissue from counts in thin sections by electron microscopy. J. Neurocytol., 3: 725-735.
3. Benes, F.M. and Lange, N. (2001) Two dimensional versus three dimensional cell counting: A practical perspective. Trends Neurosci., 24: 11-17.
4. Bloom, F.E. and Aghajanian, G.K. (1968) Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid. J. Ultrastruct. Res., 22: 361-375.
5. Clarke, P.G.H. (1992) How inaccurate is the Abercrombie correction factor for cell counts?. Trends Neurosci., 15: 211-212.
6. Clarke, P.G.H. (1993) An unbiased correction factor for cell counts in histological sections. J. Neurosci. Methods, 49: 133-140.
7. Coggeshall, R.E. and Lekan, H.A. (1996) Methods for determining numbers of cells and synapses: A case for more uniform standards of review. J. Comp. Neurol., 364: 3-16.
8. Colonnier, M. and Beaulieu, C. (1985) An empirical assessment of stereological formulae applied to the counting of synaptic discs in the cerebral cortex. J. Comp. Neurol., 231: 175-179.
9. Coupland, R.E. (1968) Determining sizes and distribution of sizes of spherical bodies such as chromaffin granules in tissue sections. Nature, 217: 384-388.
10. Erişir, A., Van Horn, S.C., Bickford, M.E. and Sherman, S.M. (1998) Distribution of synapses in the lateral geniculate nucleus of the cat. Differences between laminae A and A1 and between relay cells and interneurons. J. Comp. Neurol., 390: 247-255.
11. Floderus, S. (1944) Untersuchungen über den bau der menschlichen hypophyse mit besonderer berücksichtung der quantitativen mikromorphologischen verhältnisse. Acta Pathol. Microbiol. Scand. Suppl., 53: 1-266.
12. Geuna, S. (2000) Appreciating the difference between design-based and model-based sampling strategies in quantitative morphology of the nervous system. J. Comp. Neurol., 427: 333-339.
13. Glaser, E.M. (1982) Snell's law: The bane of computer microscopists. J. Neurosci. Methods, 5: 201-202.
14. Guillery, R.W. (1969) A quantitative study of synaptic interconnections in the laminae of the dorsal lateral geniculate nucleus of the cat. Z. Zellforsch., 96: 1-38.
15. Guillery, R.W. and Herrup, K. (1997) Quantification without pontification: Choosing a method for counting objects in sectioned tissues. J. Comp. Neurol., 386: 2-7.
16. Gundersen, H.J.G. and Østerby, R. (1981) Optimizing sampling efficiency of stereological studies in biology: Or 'do more less well!'. J. Microsc., 121: 65-73.
17. Gundersen, H.J.G., Bagger, P., Bendtsen, T.F., Evans, S.M., Korbo, L., Marcussen, N., Moller, A., Nielsen, K., Nyengaard, J.R. and Pakkenberg, B. (1988) The new stereological tools: Disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS, 96: 857-881.
18. Gundersen, H.J.G., Jensen, E.B., Kieu, K. and Nielsen, J. (1999) The efficiency of systematic sampling in stereology - Reconsidered. J. Microscop., 193: 199-211.
19. Haug, H. (1956) Remarks on the determination and significance of the gray cell coefficient. J. Comp. Neurol., 104: 473-492.
20. Haug, H. (1986) History of neuromorphometry. J. Neurosci. Methods, 18: 1-17.
21. Haug, H. (1987) Brain sizes, surfaces and neuronal sizes of the cortex cerebri: A stereological investigation of man and his variability and a comparison with some mammals. Am. J. Anat., 180: 126-142.
22. Hedreen, J.C. (1998) What was wrong with the Abercrombie and empirical cell counting methods? A review. Anat. Rec., 250: 373-380.
23. Hendry, I.A. (1976) A method to correct adequately for the change in neuronal size when estimating neuronal numbers after nerve growth factor treatment. J. Neurocytol., 5: 337-349.
24. Konigsmark, B.W. (1970) Methods for the counting of neurons. In: W.J.H. Nauta and S.O.E. Ebbesson (Eds.), Contemporary Research Methods in Neuroanatomy. Springer, Heidelberg, pp. 315-338.
25. Lange, P.W. and Edström, A. (1954) Determination of thickness of microscopic objects. Lab. Invest., 3: 116-131.
26. Lillie, R.D. (1965) Histopathologic Technic and Practical Histochemistry. 3rd Edn., McGraw Hill, New York, NY.
27. Marengo, N.P. (1944) Paraffin section thickness - A direct method of measurement. Stain Technol., 19: 1-10.
28. Mayhew, T.M. and Gundersen, H.J.G. (1996) "If you assume you can make an ass out of u and me": A decade of the disector for stereological counting of particles in 3D space. J. Anat., 188: 1-15.
29. Miller, W.S. and Carlton, E.P. (1895) The relation of the cortex of the cat's kidney to the volume of the kidney and an estimation of the number of glomeruli. Trans. Wisc. Acad. Sci., 10: 525-538.
30. Pakkenberg, B. and Gundersen, H.J.G. (1989) New stereological method for obtaining unbiased and efficient estimates of total cell number in human brain areas. Exemplified by the mediodorsal nucleus in schizophrenics. APMIS, 97: 677-681.
31. Pakkenberg, B. and Gundersen, H.J.G. (1995) Solutions to old problems in the quantitation of the central nervous system. J. Neurol. Sci., 129(Suppl 95): 65-67.
32. Pakkenberg, B. and Gundersen, H.J.G. (1997) Neocortical neuron number in humans. Effect of sex and ages. J. Comp. Neurol., 384: 312-320.
33. Small, J.V. (1968) Measurements of section thickness. Proc. 4th Eur. Cong. Electron Microsc., 1: 609.
34. Sterio, D.C. (1984) The unbiased estimation of number and sizes of arbitrary particles using the disector. J. Microscop., 134: 127-136.
35. Scott, G.L. and Guillery, R.W. (1974) Studies with the high voltage electron microscope of normal, degenerating and Golgi impregnated neural processes. J. Neurocytol., 3: 567-590.
36. Uylings, H.B., van Eden, C.G. and Hofman, M.A. (1986) Morphometry of size/volume variables and comparison of their bivariate relations in the nervous system under different conditions. J. Neurosci. Methods, 18: 19-37.
37. Weibel, E.R. (1969) Stereological principles for morphometry in electron microscopic cytology, Int. Rev. Cytol., 26: 235-302.
38. West, M.J. (1999) Stereological methods for estimating the total number of neurons and synapses: Issues of precision and bias. Trends Neurosci., 22: 51-61.
39. West, M.J. (2002) Design based stereological methods for counting neurons. In: T. Sutula and A. Pitkänen (Eds.), Do Seizures Damage the Brain. Progress in Brain Research, Vol. 135. Elsevier, Amsterdam, pp. 43-51.
40. West, M.J. and Slomianka, L. (1998) Total numbers of neurons in the layers of the human entorhinal cortex. Corrigendum. Hippocampus, 8: 426.
41. West, M.J., Ostergaard, K., Andreassen, O.A. and Finsen, B. (1996) Estimation of the number of somatostatin neurons in the striatum: An in situ hybridization study using the optical fractionator method. J. Comp. Neurol., 370: 11-22.
42. Williams, R.W. and Rakic, P. (1988) Three dimensional counting: An accurate and direct method to estimate numbers of cells in sectioned material. J. Comp. Neurol., 278: 344-352.
43. Williams, R.W., Strom, R.C., Rice, D.S. and Goldowitz, D. (1996) Genetic and environmental control of variation in retinal ganglion cell number in mice. J. Neurosci., 16: 7193-7205.