Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination

Molecular and Biochemical Parasitology

Volumn 150, Issue 1, 2006, Pages 118-121

  Maier, Alexander G ^a   Braks, Joanna A M ^b   Waters, Andrew P ^b   Cowman, Alan F ^a  

^a WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Gene knockout; Malaria; Negative selection; Transfection

Indexed keywords

4,6 DIAMINO 1,2 DIHYDRO 2,2 DIMETHYL 1 [3 (2,4,5 TRICHLOROPHENOXY)PROPOXY] 1,3,5 TRIAZINE; CYTOSINE DEAMINASE; DIHYDROFOLATE REDUCTASE; FLUCYTOSINE; FLUOROURACIL; FLUOROURIDINE; GANCICLOVIR; PYRIMETHAMINE; THYMIDINE KINASE; THYMIDYLATE SYNTHASE; URACIL PHOSPHORIBOSYLTRANSFERASE;

ANTIBIOTIC RESISTANCE; ANTIMALARIAL ACTIVITY; ARTICLE; BYSTANDER EFFECT; DNA SYNTHESIS; EPISOME; ESCHERICHIA COLI; GENE DELETION; GENETIC MANIPULATION; GENETIC TRANSFECTION; HERPES SIMPLEX VIRUS; HOMOLOGOUS RECOMBINATION; IC 50; KNOCKOUT GENE; NONHUMAN; OPEN READING FRAME; PLASMID; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROGENY; RNA SYNTHESIS; SACCHAROMYCES CEREVISIAE; SOUTHERN BLOTTING; SUICIDE GENE; TOXOPLASMA GONDII; TRANSGENE; WILD TYPE;

ANIMALS; ANTIMALARIALS; CYTOSINE DEAMINASE; FLUCYTOSINE; GENE DELETION; GENE TARGETING; HUMANS; PENTOSYLTRANSFERASES; PLASMODIUM FALCIPARUM; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SELECTION (GENETICS); TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION; TRIAZINES;

PLASMODIUM FALCIPARUM;

EID: 33748300551     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2006.06.014     Document Type: Article

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